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Polypeptide as well as phlogenzym tablet composition and application thereof

A composition and drug technology, applied in the field of preparation of the above chemical products, can solve the problems of changing the properties of active proteins, reducing the activity of proteins, increasing potential safety hazards, etc., and achieving the effects of convenient equipment, good stability, and easy popularization and application.

Active Publication Date: 2015-07-08
西安迪赛生物药业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high temperature compression usually required in the preparation of tablets, for protein-containing preparations, it is particularly easy to reduce the activity of the protein, so a larger amount of active protein needs to be added
Moreover, proteins denatured at high temperatures are more likely to cause allergic reactions, increasing potential safety hazards
[0003] For this reason, existing technologies such as Chinese Patent Application No. 200410030650.7 avoid high-temperature tableting by directly filling enteric-coated capsules, but this does not fundamentally change the properties of active proteins, especially the stability of long-term high-temperature storage

Method used

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  • Polypeptide as well as phlogenzym tablet composition and application thereof
  • Polypeptide as well as phlogenzym tablet composition and application thereof
  • Polypeptide as well as phlogenzym tablet composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of embodiment 1 polypeptide of the present invention

[0025] After long-term research, the inventors have developed the polypeptide of the present invention and its optimized expression gene sequence in yeast, wherein the nucleotide of the gene is shown in SEQ ID NO: 1, and the amino acid of the polypeptide of the present invention encoded by it is The sequence is shown in SEQ ID NO:2. We commissioned Xi'an Xintong Pharmaceutical Research Co., Ltd. to synthesize the gene and clone it into yeast cells. In short, use the upstream and downstream primers shown in SEQ ID NO: 3 and 4 to perform PCR amplification using the synthesized gene as a template , the amplified product was digested by EcoR I and Xba I and connected between the multiple cloning sites of EcoR I and Xba I of pPICZαA (available from Invitrogen). After sequencing and confirming that it was correct, the positive plasmid was transformed into yeast In the GS115 strain (available from Invitrog...

Embodiment 2

[0028] Embodiment 2 Stability study of the polypeptide of the present invention

[0029] With reference to the protease activity detection method described in Harrach et al. (J Protein Chem, 14:41-52), the polypeptide of the present invention prepared in Example 1 and the reference polypeptide prepared in high temperature storage for different times were respectively detected against the substrate L-Pyr-Phe- Hydrolytic activity of Leu-pNA. For the convenience of comparison, the activity of 1 mg of the control polypeptide stored at high temperature for 0 days (that is, taken out from the storage state at -20°C for detection immediately) was counted as 1, and the relative activities of 1 mg of different polypeptides stored under different conditions are shown in Table 1. shown.

[0030] The stability of table 1 polypeptide

[0031]

[0032] As can be seen from Table 1, the activity of an equivalent amount of polypeptide of the present invention and the wild-type control pol...

Embodiment 3 2

[0033] The preparation of embodiment 3 two enzyme tablets

[0034] Take 502 grams of starch and 130 grams of lactose, add 50% (V / V) ethanol to make a soft material, granulate, dry at 45°C until the water content is less than 5% (W / W), pass through a 20-mesh sieve, That is, the ball core is obtained.

[0035] Get 90 grams of the polypeptide of the present invention prepared in Example 1, 48 grams of trypsin and 100 grams of rutin, mix evenly with 5 grams of silicon dioxide micropowder, 25 grams of microcrystalline cellulose and the above-mentioned ball core, press into tablets, and press into 1000 piece.

[0036] Get 160 grams of oprdry standard coating powder, dissolve in 4L of 75% (V / V) ethanol, and prepare enteric coating solution. The above-mentioned formed tablets are placed in the fluidized bed, start the fluidized bed, and spray the enteric coating solution to these formed tablets with atomizing nozzles, wherein the parameters of the fluidized bed setting are: bed temp...

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Abstract

The invention provides a novel polypeptide with an amino acid sequence as shown in SEQ ID NO:2 or obtained by adding, deleting and / or substituting one or more amino acid residues on the sequence as shown in SEQ ID NO:2. The invention further provides a pharmaceutical composition comprising the polypeptide, preferably a phlogenzym tablet which is used for relieving edema after trauma or operation, shortening blood clot coagulation time, relieving pains caused by swelling and inflammation, treating thrombophlebitis, inflammation of genitourinary tract, arthritis (for example chronic arthritis) or soft tissue rheumatism.

Description

technical field [0001] The present invention belongs to the technical field of proteins or polypeptides. Specifically, the present invention relates to novel polypeptides and pharmaceutical compositions thereof, preferably also pancreatin tablets composed of the same and other active ingredients. In addition, the present invention also relates to the preparation method and medical application of the above chemical products. Background technique [0002] Phlogenzym tablets, also known as two-enzyme tablets or pancreatin tablets, whose active ingredients are mainly composed of protein, are used to reduce post-traumatic and post-operative edema, shorten blood clot aggregation time, slow down swelling and pain caused by inflammation, and can be used for such as thrombosis Treatment of diseases such as phlebitis, genitourinary tract inflammation, arthritis (eg, chronic arthritis) and soft tissue rheumatism. However, since high-temperature compression is usually required in the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50C12N15/57C12N15/63A61K38/48A61P29/00A61P9/14A61P19/02
CPCC12N9/50
Inventor 吴建中
Owner 西安迪赛生物药业有限责任公司
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