Method for preparing persimmon tannins with high micromolecular tannin content from persimmons

A technology of small molecules and persimmons, which is applied in the field of preparation of persimmon tannins with high content of small molecule tannins, which can solve problems such as the influence of sulfate radical content, cumbersome operation process, and impact on product content and yield, and achieve high-efficiency cell wall breaking , uniform particle size distribution, simple and easy operation

Inactive Publication Date: 2015-07-22
桂林得坤生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional and commonly used methods for extracting persimmon tannins with high content of small molecular tannins mainly include acid extraction, alkali extraction and water extraction, etc. Among them, hot water extraction is the most common method, and acid and alkali extraction will destroy the structure of persimmon tannins, and at the same time It has an impact on the content of sulfate radicals; while the extraction rate of hot water extraction is generally low, it is difficult to meet the production needs
Generally, after water or alcohol extraction, the extract must go through a series of processes such as adsorption, rinsing, and elution after decompression and concentration, especially repeated rinsing with distilled water until the eluate cannot detect sugar by the phenol-sulfuric acid method. So far, the operation process is cumbersome, otherwise, in the later drying process, due to the high proportion of sugar content in the persimmon tannin concentrate, the dryer "sticks to the wall" seriously, and the material cannot be dried and solidified
Chinese patent CN102550993B discloses a method for preparing astringent persimmon powder with a high content of small molecular tannins. It is to wash, crush, and squeeze the seven or eight ripe persimmons, compound enzymatic hydrolysis, color protection, filtration, and enzyme and sterilization , vacuum degassing to obtain the raw persimmon juice, and then the raw persimmon juice is subjected to microfiltration, ultrafiltration, and freeze-drying to obtain astringent persimmon powder with a high content of small molecule tannins, but this method is relatively simple for the pretreatment of raw materials of persimmons. Affect the final product content and yield

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  • Method for preparing persimmon tannins with high micromolecular tannin content from persimmons

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Embodiment 1

[0021] A method for preparing persimmon tannins with high content of small molecule tannins from persimmons, comprising the steps of:

[0022] 1) Raw material processing: 10kg of persimmons that are seven to eighty percent ripe are washed and crushed;

[0023] 2) High-pressure micro-jet micro-grinding treatment: add 50kg of pure water to the crushed persimmons, process it with a high-speed shear emulsifier for 10 minutes at a speed of 12000r / min, and then use a high-pressure micro-jet ultra-fine pulverizer to process it at 25°C , the treatment pressure is 120MPa, and the number of treatments is 3 times to obtain a paste slurry;

[0024] 3) Fermentation: The paste slurry obtained in step 2) is subjected to ultra-high temperature instantaneous sterilization, then adjusted to pH 7.0, inserted into the cultured mature Lactobacillus bulgaricus liquid strain at 10% by volume, and put into a fermenter for fermentation , the fermentation temperature is 35-37°C, and the fermentation t...

Embodiment 2

[0029] A method for preparing persimmon tannins with high content of small molecule tannins from persimmons, comprising the steps of:

[0030] 1) Raw material processing: 10kg of persimmons that are seven to eighty percent ripe are washed and crushed;

[0031] 2) High-pressure micro-jet ultra-fine pulverization treatment: add 60kg of pure water to the crushed persimmons, process them with a high-speed shear emulsifier for 20 minutes at a speed of 10,000r / min, and then use high-pressure micro-jet ultra-fine pulverization equipment for processing at 25°C , the treatment pressure is 100MPa, and the number of treatments is 2 times to obtain a paste slurry;

[0032] 3) Fermentation: The paste slurry obtained in step 2) is subjected to ultra-high temperature instantaneous sterilization, then adjusted to pH 6.5, and inserted into a cultured mature Lactobacillus acidophilus liquid strain at 12% by volume, and put into a fermenter Fermentation, the fermentation temperature is 35-37°C,...

Embodiment 3

[0037] A method for preparing persimmon tannins with high content of small molecule tannins from persimmons, comprising the steps of:

[0038] 1) Raw material processing: 10kg of persimmons that are seven to eighty percent ripe are washed and crushed;

[0039]2) High-pressure micro-jet ultra-fine pulverization treatment: add 50kg of pure water to the crushed persimmons, process them with a high-speed shear emulsifier for 15 minutes at a speed of 8000r / min, and then use high-pressure micro-jet ultra-fine pulverization equipment for processing at 25°C , the treatment pressure is 80MPa, and the number of treatments is 2 times to obtain a paste slurry;

[0040] 3) Fermentation: The paste slurry obtained in step 2) is subjected to ultra-high temperature instantaneous sterilization, placed in a sealed fermentation tank, and activated pectinase, apple amylase, and pectin lyase are added for enzymolysis. The enzymolysis time is 25 minutes, the enzymolysis temperature is 43-45°C, the ...

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Abstract

The invention discloses a method for preparing persimmon tannins with high micromolecular tannin content from persimmons. The method comprises the steps of raw material treatment, high-pressure micro-jet ultrafine crushing treatment, fermentation treatment, enzymolysis, microfiltration, ultrafiltration and drying. According to the method, a production process is improved, so that extraction time is shortened, the yield of the product is increased, the content of condensed tannins with molecular weight of 10,000-15,000 is ensured, and in addition, the content of low molecular weight tannins with molecular weight of 1,700 is also ensured.

Description

technical field [0001] The invention relates to a method for preparing persimmon tannin, in particular to a method for preparing persimmon tannin with high content of small molecule tannin from persimmon. Background technique [0002] Persimmon is a temperate deciduous fruit tree of Persimmonaceae berry class. It is native to my country and has a planting history of more than one thousand years in my country. At present, my country has the largest planting area and the largest persimmon fruit output. Persimmons are tender and juicy, sweet and refreshing, and rich in nutrition. There are two kinds of sweet persimmons and astringent persimmons. Astringent persimmons cannot be eaten directly and need to be deastringent. Sweet persimmons can be eaten fresh, or made into persimmon cakes, persimmon vinegar, persimmon wine, etc. Persimmon leaves can be made into persimmon tea. Persimmons contain a lot of tannins. Tannin, also known as tannin, is a polyphenolic polymer, which is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H13/08C07H1/08
Inventor 刘小峰何明茜罗知颂李京生刘二伟
Owner 桂林得坤生物科技股份有限公司
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