Culture medium for separating enterococcus and streptococcus from milk sample and preparation method of culture medium
A technology for streptococci and enterococci, applied in the field of culture medium for isolating bacteria from milk samples, to achieve the effect of improving sorting efficiency, obvious effect, and simplifying the process of downstream analysis and identification
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[0029] Example 2-Simultaneous sorting of Enterococcus and Streptococcus in milk-like samples:
[0030] Each 1000g medium contains: tryptone 15g, yeast extract / yeast powder 5g, beef heart extract / beef extract 3g, glucose 1.5g, sodium chloride 2.5g, disodium hydrogen phosphate 0.3g, sodium carbonate 2.0g, Nalidixic acid 18mg, polymyxin B 1.8mg, crystal violet 0.15mg, esculin 4g, ferric citrate 0.3g, agar 12g, of which glucose and polymyxin B need to be pre-prepared, filtered and sterilized, Store in aliquots at -20°C, and take it for use as needed; add water, heat and stir to dissolve the rest of the ingredients, boil for 3 minutes, and sterilize at 115°C for 12 minutes. When the temperature drops to 50°C—65°C, add the Mix the prepared glucose and polymyxin, fill it in a petri dish, and use it when the temperature drops below 37°C (to room temperature), or perform aseptic operation. Use a sealing film or sterile newspaper for this plate Wait for sealing, temporarily store at 2-8℃ ...
Example Embodiment
[0032] Example 3-For simultaneous sorting of Enterococcus and Streptococcus in the milk sample:
[0033] Each 1000g medium contains: 25g monthly peptone, 2g yeast extract / yeast powder, 2g beef extract, 1g glucose, 2g sodium chloride, disodium hydrogen phosphate 0.5g, sodium carbonate 2.6g, nalidixic acid 12mg, Polymyxin B 2mg, crystal violet 0.3mg, esculin 2g, ferric citrate 0.8g, and agar 18g. The preparation and storage methods are the same as in Example 1.
Example Embodiment
[0034] Example 4-For simultaneous sorting of Enterococcus and Streptococcus in milk-like samples:
[0035] Each 1000g medium contains: peptone 15g, yeast extract / yeast powder 5g, beef extract 5g, glucose 3g, sodium chloride 2.5g, disodium hydrogen phosphate 0.4g, sodium carbonate 3g, nalidixic acid 20mg, polyviscous Bacteriocin B is 1.2 mg, crystal violet 0.1 mg, esculin 5 g, ferric citrate 0.5 g, and agar 10 g. The preparation and preservation methods are the same as in Example 1.
[0036] Example 5-Separate and simultaneous separation of Enterococcus in the milk sample: The difference from Example 1 is that the sodium chloride is 65 g, and the sorting culture temperature is 45°C.
[0037] Example 6-Separate and simultaneous separation of Enterococcus in the milk sample: The difference from Example 1 is that the sodium chloride is 70 g, and the sorting culture temperature is 47°C.
[0038] Example 5-Separate and simultaneous separation of Enterococcus in the milk sample: The differe...
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