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Preparation method of human serum albumin gold nanoclusters

A technology of human serum albumin and albumin gold, which is applied in the field of preparation of gold nanoclusters of human serum albumin, can solve the problems of biocompatibility, poor stability, toxicity, damage, etc. The effect of small diameter and uniform distribution

Inactive Publication Date: 2015-07-29
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, some traditional fluorescence imaging diagnostic materials are easily bleached under certain light excitation, and have toxicity in composition, poor biocompatibility and stability. While performing diagnostic imaging on tumors, they also cause serious damage to other normal tissues.

Method used

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  • Preparation method of human serum albumin gold nanoclusters
  • Preparation method of human serum albumin gold nanoclusters
  • Preparation method of human serum albumin gold nanoclusters

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Utilize ultrasonic optimization to prepare the method for the gold nanocluster (HSA-AuNCs) of template, it comprises the steps:

[0025] 1) Slowly add 1.03mL chloroauric acid solution (10g / L) into 2.5mL human serum albumin (HSA) solution (50mg / mL), stir vigorously and mix evenly, then add 0.25mL 1mol / L NaOH, stir After a few minutes of thorough mixing, apply ultrasound with a power of 150W at room temperature, and set the on-off time ratio of the ultrasound to 5:5, that is, the ultrasound is turned on for 5 minutes and turned off for 5 minutes. Brown gold cluster solution;

[0026] 2) Take 3ml of the prepared gold cluster solution and put it into a glass bottle, and quickly inject 3ml of 10g / L ZnCl 2 Solution, conjugated precipitate to obtain a turbid solution, centrifuged at 2000rpm for 15min; then the precipitate was washed with distilled water, and the whole process was repeated three times; then the precipitate was redissolved in 1ml PBS (PH=7.4) buffer, and dialyz...

Embodiment 2

[0028] Utilize ultrasonic optimization to prepare the method for the gold nanocluster (HSA-AuNCs) of template, it comprises the steps:

[0029] 1) Add 1.03mL of chloroauric acid solution (10g / L) into 2.5mL of human serum albumin (HSA) solution (50mg / mL), stir vigorously and mix evenly, then add 0.25mL of 1mol / L NaOH, stir for several After 1 minute of thorough mixing, at room temperature, apply ultrasound with a power of 150W, set the on-off time ratio of the ultrasound to 6:4, that is, turn on the ultrasound for 6 minutes, and turn it off for 4 minutes, and repeat the cycle several times. After one hour of ultrasound, a brown solution is obtained;

[0030] 2) Take 3ml of the prepared gold cluster solution and put it into a glass bottle, and quickly inject 3ml of 10g / L ZnCl 2 Solution, conjugated precipitate to obtain a turbid solution, centrifuged at 2000rpm for 15min, then took the precipitate and washed it with distilled water, the whole process was repeated three times; th...

Embodiment 3

[0032] Utilize ultrasonic optimization to prepare the method for the gold nanocluster (HSA-AuNCs) of template, it comprises the steps:

[0033] 1) Add 1.03mL of chloroauric acid solution (10g / L) into 2.5mL of human serum albumin (HSA) solution (50mg / mL), stir vigorously and mix evenly, then add 0.25mL of 1mol / L NaOH, stir for several After 1 minute of thorough mixing, apply ultrasound with a power of 150W at room temperature, set the on-off time ratio of the ultrasound to 7:3, that is, turn on the ultrasound for 7 minutes and turn it off for 3 minutes, and repeat the cycle several times. Ultrasonic for one hour to obtain a brown solution;

[0034] 2) Take 3ml of the prepared gold cluster solution and put it into a glass bottle, and quickly inject 3ml of 10g / L ZnCl 2 Solution, conjugated precipitate to obtain a turbid solution, centrifuged at 2000rpm for 15min; then precipitated, washed with distilled water, and the whole process was repeated three times; then the precipitate w...

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Abstract

The invention belongs to the technical field of nano materials, and specifically relates to a preparation method of human serum albumin gold nanoclusters. The method comprises the steps of 1) slowly adding a chloroauric acid solution to a human serum albumin solution, highly agitating, then adding a NaOH solution to regulate the pH value to be 11 to 12.5, and ultrasonically processing the mixed solution according to selected ultrasonic opening-closing ratio under the room temperature of 37 DEG C so as to obtain a gold nanoclusters solution; 2) performing conjugating precipitation for the gold nanoclusters solution and an ZnCl2 solution, centrifuging, and washing the precipitate through distilled water, dissolving the precipitate into a PBS buffer solution again, respectively dialyzing in the PBS buffer solution and deionized water to obtain a human serum albumin gold nanoclusters solution. According to the method, human serum albumin is used to replace heterologous protein to be used as a protecting agent and a reducing agent to prepare the gold nanoclusters, so that the immunogenicity of a human body can be removed; gold nanoclusters being small in particle size, uniform in distribution, high in quantum output and high in near infrared fluorescence can be prepared within a short time.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a preparation method of human serum albumin gold nanoclusters. Background technique [0002] Cancer, also known as malignant tumor, seriously threatens human health and life, and is still regarded as an incurable disease in today's medicine. In the process of cancer treatment, effective early diagnosis and real-time tracking of tumor cells are important means to improve their cure rate and survival rate. However, some traditional fluorescent imaging diagnostic materials are easily bleached under certain light excitation, and have toxicity in composition, poor biocompatibility and stability. While performing diagnostic imaging on tumors, they also cause serious damage to other normal tissues. . [0003] With the rapid development of nanotechnology, the relationship between nanomaterials and medicine has become increasingly close. Gold nanoclusters are a new ty...

Claims

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Application Information

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IPC IPC(8): B22F9/24C09K11/58B82Y40/00
Inventor 黄进张娣黄海涛原弘夏涛余家会
Owner WUHAN UNIV OF TECH
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