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Method for accelerating microbial fermentation by negative ion powder

A technology of microbial fermentation and negative ion powder, which is applied in the field of fermentation engineering, can solve the problems of inhibiting growth and affecting the mass transfer of oxygen nutrients, and achieve the effects of increasing biomass, saving energy consumption, and shortening the fermentation cycle

Inactive Publication Date: 2015-07-29
QUANZHOU NAISI MATERIAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To increase dissolved oxygen, the stirring speed needs to be increased. The higher the speed, the higher the requirements for the equipment. The power of the motor must be large enough, the tank body must be stable enough, the frequency of seal replacement will be higher, and the generation of a large number of bubbles in the tank and the loss of the defoamer Feed feeding will affect the transfer of oxygen and mass transfer of nutrients, thereby inhibiting the growth of bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Add culture medium to a 1L Erlenmeyer flask, then insert Bacillus subtilis into the flask, and culture it on a shaker at 150 rpm at 32°C for 36 h to obtain a shake flask seed culture solution. Add 7 L of medium to a 10 L fermenter in proportion, add negative ion powder with a particle size of 0.2 μm at a ratio of 1% (g / mL), autoclave for 30 min, and inoculate the seeds with an inoculation amount of 9% of the fermentation broth. The culture solution was put into a fermenter, and the conventional high-density fermentation of Bacillus subtilis was used as a control. Adjust the pH value to 7.2, keep the tank temperature at 37°C, the tank pressure at 30 kap, the stirring speed at 100 rpm, the ventilation rate at 1:0.3, the fermentation time for 32 hours, put the tank at the end of the fermentation time, and filter the bacteria and fermentation broth. Compared with the control group, negative ion powder significantly improved the cell morphology of Bacillus subtilis, and the ...

Embodiment 2

[0020] Put the culture medium in a 1L Erlenmeyer flask, then insert Bacillus subtilis into the flask, and culture it on a shaker at 150 rpm at 32°C for 36 h to obtain a shake flask seed culture solution. In a 10 L fermenter, add 7 L of medium in proportion, add negative ion powder with a particle size of 15 μm at a ratio of 0.2% (g / mL), autoclave for 30 min, and inoculate the seed culture solution with an inoculation amount of 9% of the fermentation solution. Insert it into a fermenter, and take conventional high-density fermentation of Bacillus subtilis as a control. Adjust the pH value to 7.2, keep the tank temperature at 37°C, the tank pressure at 30 kap, the stirring speed at 100 rpm, the ventilation rate at 1:0.3, the fermentation time for 32 hours, put the tank at the end of the fermentation time, and filter the bacteria and fermentation broth. Compared with the control group, negative ion powder significantly improved the cell morphology of Bacillus subtilis, and the ce...

Embodiment 3

[0022] Put the culture medium in a 1L Erlenmeyer flask, then insert Chlorella into the flask, and culture it on a shaker at 180 rpm at 25°C for 2 days to obtain a shaker flask seed culture solution. Add 10L of medium in proportion to 15L fermenter, add negative ion powder with a particle size of 0.1μm at a ratio of 1% (g / mL), autoclave for 30 min, and inoculate the seeds with an inoculation amount of 15% of the fermentation broth. The culture solution was put into the fermenter, and the high-density fermentation of conventional chlorella was used as the control. Adjust the pH value to 6.5, keep the tank temperature at 28°C, the tank pressure at 30 kap, the stirring speed at 100 rpm, the ventilation rate at 1:0.4, the fermentation time is 7 days, put the tank at the end of the fermentation time, and filter the algae cells and fermentation broth. An appropriate amount of algae cells was dried and weighed to obtain biomass, and the biodiesel in the algae cells was extracted by th...

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Abstract

The invention discloses a method for accelerating microbial fermentation by negative ion powder. The method comprises specific steps as follows: (1), a culture medium is put in a 1L triangular flask, bacillus subtilis or chlorella is inoculated and cultured for 1-3 days on a shaking bed at the temperature of 20-37 DEG C at a speed of 140-210 rpm, and a shake flask seed culture solution is obtained; (2), the culture medium and the negative ion powder are added proportionally to a fermentation tank and sterilized under the high pressure for 30 min, and the seed culture solution is inoculated to the fermentation tank; (3), the temperature of the tank is kept at 20 DEG C-37 DEG C, the tank pressure is 15-60 kap, the stirring speed is 80-130 rpm, the ventilation ratio is 1: (0.1-0.5), the fermentation culture time is 1-8 days, and bacterial strains and a fermentation liquid are filtered after being released from the tank. The negative ion powder capable of promoting rapid growth of microorganisms is added in a conventional fermentation tank, the growth speed of the microorganisms can be increased, accumulation of biomass and products is increased, the fermentation period is shortened, a buffer function is performed on pH of the solution, the quantity of demanded dissolved oxygen in the fermentation tank is guaranteed without increase of tank pressure or introduction of pure oxygen, and the negative ion powder can be recycled after fermentation ends.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, and in particular relates to a method for promoting microbial fermentation by negative ion powder. Background technique [0002] With the advancement of human science, the research on microorganisms has become more and more in-depth, and the role of microorganisms in the fields of industry, agriculture, medicine, food, energy and other fields has become more and more eye-catching, especially the construction of a circular economy cannot do without The involvement of microorganisms and the development and innovation of applied technologies. Microbial growth efficiency is high, energy consumption is low, and a large amount of beneficial biologically active substances are produced, which has wide application prospects and high economic value. Fermentation cycle, reducing hydropower usage is still a constraint factor for microbial fermentation. [0003] Most microorganisms are aerobic, inclu...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/20C12N9/28C12N1/12C12P7/64C12R1/125C12R1/89
CPCC12N1/38C12N9/2417C12P7/649C12Y302/01001Y02E50/10
Inventor 陈丽琴王珊珊陈昕雄陈嘉炼
Owner QUANZHOU NAISI MATERIAL TECH