Method for producing citric acid by using lignocellulose raw material

A technology of lignocellulose and citric acid, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of non-industrialization, low citric acid output, etc., and achieves low cost, low substrate cost, A wide range of effects

Active Publication Date: 2015-07-29
CATHAY R&D CENT CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low yield of citric acid, this process does not have the premise of industrialization

Method used

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  • Method for producing citric acid by using lignocellulose raw material
  • Method for producing citric acid by using lignocellulose raw material

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Embodiment 1

[0032] Mix the dried corn stalks with 5% dilute sulfuric acid solution at a mass ratio of 2:1, and then pretreat them at 190°C and 1.2MPa for 3 minutes; The used bacterial species Amorphotheca resinae ZN1 (its preservation number CGMCC7452) was detoxified in a solid state at 25°C for 10 days; the detoxified material was added to a fermenter, and the solid content was adjusted to 25%, and the amount of cellulase was 15 FPU / gram of dry solid, Saccharification at 50°C and pH 4.8 for 48 hours; then the supernatant obtained by centrifugation is the straw enzymatic hydrolysis solution. Add 2.5g / L ammonium chloride, potassium dihydrogen phosphate 2.5g / L, magnesium sulfate heptahydrate 0.25g / L, copper sulfate pentahydrate 0.25mg / L, zinc sulfate heptahydrate 1.1mg / L to the enzymolysis solution, Ferrous sulfate heptahydrate 6.5mg / L, manganese chloride 3.6mg / L, and insert cultured Aspergillus niger M288 (purchased from Shanghai Institute of Industrial Microbiology Culture Collection Cent...

Embodiment 2

[0034] Mix the dried corn stalks with 5% dilute sulfuric acid solution at a mass ratio of 2:1, and then pretreat them at 190°C and 1.2MPa for 3 minutes; The used bacterial species Amorphotheca resinae ZN1 (its preservation number CGMCC7452) was detoxified in a solid state at 25°C for 10 days; the detoxified material was added to a fermenter, and the solid content was adjusted to 25%, and the amount of cellulase was 15 FPU / gram of dry solid, Saccharification at 50°C and pH 4.8 for 48 hours; then the supernatant obtained by centrifugation is the straw enzymatic hydrolysis solution. Add 1.0g / L ammonium chloride, potassium dihydrogen phosphate 2.5g / L, magnesium sulfate heptahydrate 0.25g / L, copper sulfate pentahydrate 0.25mg / L, zinc sulfate heptahydrate 1.1mg / L to the enzymolysis solution, Ferrous sulfate heptahydrate 6.5mg / L, manganese chloride 3.6mg / L, and insert cultured Aspergillus niger M288 (purchased from Shanghai Institute of Industrial Microbiology Culture Collection Cent...

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Abstract

The invention relates to a method for producing citric acid by using a lignocellulose raw material. One method is by culturing Aspergillus niger M288 in liquid, and then inoculating to in an enzymatic hydrolysate of cellulose raw material, so as to convert the glucose in enzymatic hydrolysate into citric acid by Aspergillus niger. The other method is by first culturing Aspergillus niger M288 in liquid, and then inoculating Aspergillus niger into a lignocellulose fermenting tank containing a certain content of solid, and adding cellulase at the same time, so as to conduct enzymolysis on lignocellulose by cellulase and ferment the generated sugar into citric acid by Aspergillus niger at the same time. The method uses lignocellulose as raw material for the production of citric acid; the process is simple and low in cost; and especially, the usage of lignocellulose as raw material has broad application and development prospects.

Description

【Technical field】 [0001] The invention relates to the field of bio-manufacturing and bio-refining of bio-based chemicals, in particular, it uses lignocellulosic raw materials such as corn stalks to produce citric acid through step-by-step saccharification and fermentation or synchronous saccharification and fermentation. 【Background technique】 [0002] Citric acid, also known as citric acid, is one of the main metabolites of living organisms. Widely used in food, medicine, chemical industry and other fields, it is one of the important organic acids. At present, more than 90% of citric acid in the world is produced by fermentation of Aspergillus niger. The main raw materials for citric acid production in my country include starch, glucose, corn flour, rice flour, cassava flour, etc. Most of the industrial production now uses dried potatoes and corn flour as raw materials, while European and American countries use glucose, starch and other concentrates for fermentation. Duri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/48C12R1/685
CPCC12P7/48C12P19/02C12P19/14C12P2201/00C12P2203/00
Inventor 鲍杰孟佼张建
Owner CATHAY R&D CENT CO LTD
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