Method for producing citric acid by using lignocellulose raw material

Abstract

The invention relates to a method for producing citric acid by using a lignocellulose raw material. One method is by culturing Aspergillus niger M288 in liquid, and then inoculating to in an enzymatic hydrolysate of cellulose raw material, so as to convert the glucose in enzymatic hydrolysate into citric acid by Aspergillus niger. The other method is by first culturing Aspergillus niger M288 in liquid, and then inoculating Aspergillus niger into a lignocellulose fermenting tank containing a certain content of solid, and adding cellulase at the same time, so as to conduct enzymolysis on lignocellulose by cellulase and ferment the generated sugar into citric acid by Aspergillus niger at the same time. The method uses lignocellulose as raw material for the production of citric acid; the process is simple and low in cost; and especially, the usage of lignocellulose as raw material has broad application and development prospects.

Description

【Technical field】

[0001] The invention relates to the field of bio-manufacturing and bio-refining of bio-based chemicals, in particular, it uses lignocellulosic raw materials such as corn stalks to produce citric acid through step-by-step saccharification and fermentation or synchronous saccharification and fermentation. 【Background technique】

[0002] Citric acid, also known as citric acid, is one of the main metabolites of living organisms. Widely used in food, medicine, chemical industry and other fields, it is one of the important organic acids. At present, more than 90% of citric acid in the world is produced by fermentation of Aspergillus niger. The main raw materials for citric acid production in my country include starch, glucose, corn flour, rice flour, cassava flour, etc. Most of the industrial production now uses dried potatoes and corn flour as raw materials, while European and American countries use glucose, starch and other concentrates for fermentation. Duri...

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