Method for preparing vascular tissue engineering stent material carried with pravastatin sodium

A technology of pravastatin sodium and scaffold material, which is applied in the field of preparation of vascular tissue engineering scaffold material loaded with pravastatin sodium, can solve the problems of non-degradable, only 1-7 days, non-degradable, etc. Effects of surgical complications, prevention of recurrence, and avoidance of acidic environment

Inactive Publication Date: 2015-08-19
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the patent uses statin molecular prodrugs, which need to be hydrolyzed into statins in the body by ester hydrolase to exert their efficacy. into the material, because the material cannot be degraded in the body, it needs to be taken out again
The patent application with the publication number CN 101856342A discloses a pravastatin transdermal formulation, which stirs pravastatin with a transdermal enhancer, a reservoir matrix composed of synthetic polymers and / or modified cellulose The drug reservoir is uniformly obtained to prepare a transdermal drug delivery preparation, but the materials used in this dosage form are also basically non-degradable materials, and the sustained drug release time is only 1 to 7 days

Method used

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  • Method for preparing vascular tissue engineering stent material carried with pravastatin sodium
  • Method for preparing vascular tissue engineering stent material carried with pravastatin sodium

Examples

Experimental program
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Effect test

Embodiment 1

[0027] 1. Preparation of scaffold materials for vascular tissue engineering

[0028] (1) Preparation of pravastatin-loaded chitosan microspheres

[0029] (1.1) Mix Tween-80 and liquid paraffin according to the volume ratio of Tween-80: liquid paraffin = 2: 100, and mix evenly under electric stirring at a speed of 500 r / min to obtain an oil phase;

[0030] (1.2) join the chitosan that viscosity is lower than 200mPa.s in the acetic acid solution that volume concentration is 1%, magnetic stirring 1h, be made into the chitosan solution that mass concentration is 1.5%; Dissolve pravastatin sodium in In the water, make the concentration of pravastatin sodium in water be 66.6g / L, obtain the pravastatin sodium aqueous solution; Then, mix the chitosan solution and the pravastatin sodium aqueous solution, stir well, make the content of mass ratio Be the aqueous phase of pravastatin sodium: chitosan=1:1;

[0031] (1.3) dissolving genipin in 30% aqueous ethanol, so that the concentratio...

Embodiment 2

[0046] 1. Preparation of scaffold materials for vascular tissue engineering

[0047] (1) Preparation of pravastatin-loaded chitosan microspheres

[0048] (1.1) Mix span-80 and ethyl benzoate according to the volume ratio of span-80: ethyl benzoate = 8: 100, and mix evenly under electric stirring at a speed of 1000r / min to obtain an oil phase;

[0049] (1.2) Adding chitosan with a viscosity of 200 to 400mPa.s into an acetic acid solution with a volume concentration of 0.5%, magnetic stirring for 5h, and preparing a chitosan solution with a mass concentration of 3%; dissolving pravastatin sodium In water, make the concentration of pravastatin sodium in water be 16.6g / L, obtain pravastatin sodium aqueous solution; Then, mix the prepared chitosan solution and pravastatin sodium aqueous solution, stir well, obtain the Ratio is the aqueous phase of pravastatin sodium: chitosan=1:4;

[0050] (1.3) Genipin is dissolved in 40% aqueous ethanol, so that the concentration of genipin in ...

Embodiment 3

[0062] 1. Preparation of scaffold materials for vascular tissue engineering

[0063] (1) Preparation of pravastatin-loaded chitosan microspheres

[0064] (1.1) Mix span-80 and liquid paraffin according to the volume ratio of span-80: liquid paraffin = 4: 100, and mix evenly under electric stirring at a speed of 850r / min to obtain an oil phase;

[0065] (1.2) Adding chitosan with a viscosity of 200 to 400mPa.s into an acetic acid solution with a volume concentration of 1%, magnetic stirring for 1h, and preparing a chitosan solution with a mass concentration of 1.5%; dissolving pravastatin sodium In water, make the concentration of pravastatin sodium in water be 33.3g / L, obtain pravastatin sodium aqueous solution; Ratio is the aqueous phase of pravastatin sodium: chitosan=1:2;

[0066] (1.3) Genipin is dissolved in 90% aqueous ethanol, so that the concentration of genipin in ethanol with a volume concentration of 90% is 13g / L to obtain a crosslinking agent solution;

[0067] ...

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Abstract

The invention relates to a method for preparing a vascular tissue engineering stent material carried with pravastatin sodium. The method consists of the following steps: (1) preparing chitosan solution and gelatin salutation; (2) preparing cross-linking agent solution; (3) mixing the chitosan solution, the gelatin salutation and the cross-linking agent solution, then adding chitosan microspheres entrapping pravastatin sodium, performing uniform mixing, pouring the mixture into a vascular stent mold, performing freeze-drying and then performing demolding; (4) repetitively washing the vascular stent after demolding and then performing freeze-drying. The vascular tissue engineering stent material carried with pravastatin sodium prepared by adopting the method has a pore diameter of 10-600mu m, the chitosan microspheres entrapping pravastatin sodium are well dispersed in the stent and the release time reaches more than 60 days.

Description

technical field [0001] The invention relates to a prosthesis capable of being transplanted into the body, in particular to a preparation method of a vascular stent material loaded with pravastatin. Background technique [0002] Cardiovascular disease has a high recurrence rate, many complications, and its incidence is increasing year by year. It has become the leading cause of death for human beings. Cardiovascular diseases with severe clinical damage often require surgical treatment, and the damaged blood vessels often need to be reconstructed and replaced. The source is usually autograft, allograft or xenograft, and artificial material replacement. Although the effect of autologous transplantation is good, the supply is insufficient. Some patients, especially those with vascular diseases or who have undergone surgery, do not have suitable autologous blood vessels; allogeneic or xenotransplantation has immune rejection, infection, and ethical issues. Artificial materials s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/24A61L27/20A61L27/54A61L27/56
Inventor 卢玲冯婕刘瑞源刘中秋游文玮席华松
Owner SOUTHERN MEDICAL UNIVERSITY
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