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A preparation method of vascular tissue engineering scaffold material loaded with pravastatin sodium

A technology of pravastatin sodium and scaffold material, which is applied in the field of preparation of vascular tissue engineering scaffold materials loaded with pravastatin sodium, can solve the problems of non-degradable, only 1 to 7 days, non-degradable, etc., and achieves prevention and treatment. Efficacy of recurrence, avoidance of acidic environment, and reduction of surgical complications

Inactive Publication Date: 2017-06-13
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the patent uses statin molecular prodrugs, which need to be hydrolyzed into statins in the body by ester hydrolase to exert their efficacy. into the material, because the material cannot be degraded in the body, it needs to be taken out again
The patent application with the publication number CN101856342A discloses a pravastatin transdermal administration preparation, which mixes pravastatin with a transdermal enhancer, a reservoir matrix composed of synthetic polymers and / or modified cellulose Obtain drug reservoirs and prepare transdermal drug formulations, but the materials used in this dosage form are also basically non-degradable materials, and the sustained drug release time is only 1 to 7 days

Method used

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  • A preparation method of vascular tissue engineering scaffold material loaded with pravastatin sodium
  • A preparation method of vascular tissue engineering scaffold material loaded with pravastatin sodium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Preparation of scaffold materials for vascular tissue engineering

[0028] (1) Preparation of pravastatin-loaded chitosan microspheres

[0029] (1.1) Mix Tween-80 and liquid paraffin according to the volume ratio of Tween-80: liquid paraffin = 2: 100, and mix evenly under electric stirring at a speed of 500 r / min to obtain an oil phase;

[0030] (1.2) join the chitosan that viscosity is lower than 200mPa.s in the acetic acid solution that volume concentration is 1%, magnetically stir 1h, be made into the chitosan solution that mass concentration is 1.5%; Dissolve pravastatin sodium in In the water, make the concentration of pravastatin sodium in water be 66.6g / L, obtain the pravastatin sodium aqueous solution; Then, mix the chitosan solution prepared with the pravastatin sodium aqueous solution, stir, and obtain the mass ratio Be the aqueous phase of pravastatin sodium: chitosan=1:1;

[0031] (1.3) Dissolving genipin in 30% ethanol aqueous solution, so that the con...

Embodiment 2

[0046] 1. Preparation of scaffold materials for vascular tissue engineering

[0047] (1) Preparation of pravastatin-loaded chitosan microspheres

[0048] (1.1) Mix span-80 and ethyl benzoate according to the volume ratio of span-80: ethyl benzoate = 8: 100, and mix evenly under electric stirring at a speed of 1000r / min to obtain an oil phase;

[0049] (1.2) Adding chitosan with a viscosity of 200 to 400mPa.s into an acetic acid solution with a volume concentration of 0.5%, magnetic stirring for 5h, and preparing a chitosan solution with a mass concentration of 3%; dissolving pravastatin sodium In water, make the concentration of pravastatin sodium in water be 16.6g / L, obtain pravastatin sodium aqueous solution; Then, mix the prepared chitosan solution and pravastatin sodium aqueous solution, stir well, make the Ratio is the aqueous phase of pravastatin sodium: chitosan=1:4;

[0050] (1.3) Genipin is dissolved in 40% aqueous ethanol, so that the concentration of genipin in 40...

Embodiment 3

[0062] 1. Preparation of scaffold materials for vascular tissue engineering

[0063] (1) Preparation of pravastatin-loaded chitosan microspheres

[0064] (1.1) Mix span-80 and liquid paraffin according to the volume ratio of span-80: liquid paraffin = 4: 100, and mix evenly under electric stirring at a speed of 850r / min to obtain an oil phase;

[0065] (1.2) Adding chitosan with a viscosity of 200 to 400mPa.s into an acetic acid solution with a volume concentration of 1%, magnetic stirring for 1h, and preparing a chitosan solution with a mass concentration of 1.5%; dissolving pravastatin sodium In water, make the concentration of pravastatin sodium in water be 33.3g / L, obtain pravastatin sodium aqueous solution; Ratio is the aqueous phase of pravastatin sodium: chitosan=1:2;

[0066] (1.3) Genipin is dissolved in 90% aqueous ethanol, so that the concentration of genipin in ethanol with a volume concentration of 90% is 13g / L to obtain a crosslinking agent solution;

[0067] ...

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Abstract

The invention relates to a preparation method of a vascular tissue engineering scaffold material loaded with pravastatin sodium. The method is composed of the following steps: (1) preparing a chitosan solution and a gelatin solution; (2) preparing a crosslinking agent solution; ( 3) Chitosan solution, gelatin solution and cross-linking agent solution are mixed, then add the chitosan microspheres loaded with pravastatin sodium and mix evenly and pour into the vascular stent mold, demold after freeze-drying; (4) Demoulding The molded vascular stent is washed repeatedly, and then freeze-dried. The vascular tissue engineering scaffold material loaded with pravastatin prepared by the invention has a pore size of 10-600 μm, the chitosan microspheres loaded with pravastatin sodium are well dispersed in the scaffold, and the drug release time is as long as more than 60 days.

Description

technical field [0001] The invention relates to a prosthesis capable of being transplanted into the body, in particular to a preparation method of a vascular stent material loaded with pravastatin. Background technique [0002] Cardiovascular disease has a high recurrence rate, many complications, and its incidence is increasing year by year. It has become the leading cause of death for human beings. Cardiovascular diseases with severe clinical damage often require surgical treatment, and the damaged blood vessels often need to be reconstructed and replaced. The source is usually autograft, allograft or xenograft, and artificial material replacement. Although the effect of autologous transplantation is good, the supply is insufficient. Some patients, especially those with vascular diseases or who have undergone surgery, do not have suitable autologous blood vessels; allogeneic or xenotransplantation has immune rejection, infection, and ethical issues. Artificial materials s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/26A61L27/24A61L27/20A61L27/54A61L27/56
Inventor 卢玲冯婕刘瑞源刘中秋游文玮席华松
Owner SOUTHERN MEDICAL UNIVERSITY
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