Method for extracting phycocyanobilin from spirulina
A technology of phycocyanin and extraction method, which is applied in the field of extracting phycocyanin, can solve the problems of low recovery rate of phycocyanin, secondary pollution of products, and poor product quality, so as to reduce secondary pollution and product pollution , the effect of improving the recovery rate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Example Embodiment
[0018] Example one
[0019] An extraction method for extracting algae blue pigment from spirulina, the process steps of the preparation method are:
[0020] A. Dissolve fresh spirulina in sterile deionized water at a ratio of 1:10, freeze-thaw repeatedly three times;
[0021] B. Add the spirulina liquid to the high-pressure homogenizer, and homogenize it in 2 cycles at 20Kpa;
[0022] C. Add the homogenized spirulina solution to the ultrasonic breaker and break the algal cell wall with ultrasonic waves to dissolve the phycobiliprotein; extract the liquid;
[0023] D. Centrifuge the extracted material solution treated with the wall in a butterfly centrifuge for 20 minutes to remove cell debris. The aqueous phase is the phycocyanin extract; the centrifuge has a centrifugal force of 3500×g.
[0024] E. The phycocyanin extract enters the tubular ultrafiltration membrane filtration system to concentrate the extract to obtain algae slurry; the membrane molecular weight cut-off is less than 150...
Example Embodiment
[0026] Example two
[0027] An extraction method for extracting algae blue pigment from spirulina, the process steps of the preparation method are:
[0028] A. Dissolve fresh spirulina in sterile deionized water at a ratio of 1:30 and freeze and thaw three times repeatedly;
[0029] B. Add the spirulina liquid to the high-pressure homogenizer, and homogenize it in 3 cycles at 40Kpa;
[0030] C. Add the homogenized spirulina solution to the ultrasonic breaker and break the algal cell wall with ultrasonic waves to dissolve the phycobiliprotein; extract the liquid;
[0031] D. Centrifuge the extracted material solution treated with the wall in a butterfly centrifuge for 40 minutes to remove cell debris. The aqueous phase is the phycocyanin extract; the centrifuge has a centrifugal force of 4000×g.
[0032] E. The phycocyanin extract enters the tubular ultrafiltration membrane filtration system to concentrate the extract to obtain algal slurry; the membrane molecular weight cut-off is less t...
Example Embodiment
[0034] Example three
[0035] An extraction method for extracting algae blue pigment from spirulina, the process steps of the preparation method are:
[0036] A. Dissolve fresh spirulina in sterile deionized water at a ratio of 1:20, and freeze and thaw three times repeatedly;
[0037] B. Add the spirulina liquid to the high-pressure homogenizer, and homogenize it in 3 cycles at 30Kpa;
[0038] C. Add the homogenized spirulina solution to the ultrasonic breaker and break the algal cell wall with ultrasonic waves to dissolve the phycobiliprotein; extract the liquid;
[0039] D. Centrifuge the extracted material solution treated with the wall in a butterfly centrifuge for 30 minutes to remove cell debris. The aqueous phase is the phycocyanin extract; the centrifuge has a centrifugal force of 5000×g.
[0040] E. The phycocyanin extract enters the tubular ultrafiltration membrane filtration system to concentrate the extract to obtain algae slurry; the membrane molecular weight cut-off is les...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2023 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap