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Method for extracting phycocyanobilin from spirulina

A technology of phycocyanin and extraction method, which is applied in the field of extracting phycocyanin, can solve the problems of low recovery rate of phycocyanin, secondary pollution of products, and poor product quality, so as to reduce secondary pollution and product pollution , the effect of improving the recovery rate

Inactive Publication Date: 2015-08-19
JIANGXI ZHONGZAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, my country's spirulina factories have developed rapidly. The phycocyanin produced by them is extracted from spirulina algae powder. During the production process, buffer solution is used as the extraction solution, which causes product pollution. Separation by column chromatography and other methods is likely to cause secondary pollution of the product, the recovery rate of phycocyanin is low, and the product quality is poor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The extraction method of extracting phycocyanin from spirulina, the processing steps of the preparation method are:

[0020] A. Dissolve fresh spirulina in sterilized deionized water at a ratio of 1:10, and freeze and thaw repeatedly three times;

[0021] B. Add the spirulina liquid to the high-pressure homogenizer, and homogenize in 2 cycles under the state of 20Kpa;

[0022] C. Add the homogenized spirulina liquid to the ultrasonic breaker and use ultrasonic waves to break the algae cell wall to dissolve the phycobiliprotein; extract the feed liquid;

[0023] D. Centrifuge the extracted feed liquid treated by the wall in a butterfly centrifuge for 20 minutes to remove cell debris, and the water phase is the phycocyanin extract; the centrifugal force of the centrifuge is 3500×g.

[0024] E. The phycocyanin extract enters the tubular ultrafiltration membrane filtration system to concentrate the extract to obtain algal pulp; the membrane molecular weight cut-off is less...

Embodiment 2

[0027] The extraction method of extracting phycocyanin from spirulina, the processing steps of the preparation method are:

[0028] A. Dissolve fresh spirulina in sterilized deionized water at a ratio of 1:30, and freeze and thaw repeatedly three times;

[0029] B. Add the spirulina liquid to the high-pressure homogenizer, and homogenize it 3 times under the state of 40Kpa;

[0030] C. Add the homogenized spirulina liquid to the ultrasonic breaker and use ultrasonic waves to break the algae cell wall to dissolve the phycobiliprotein; extract the feed liquid;

[0031] D. Centrifuge the extracted feed solution treated by the wall in a butterfly centrifuge for 40 minutes to remove cell debris, and the water phase is the phycocyanin extract; the centrifugal force of the centrifuge is 4000×g.

[0032] E. The phycocyanin extract enters the tubular ultrafiltration membrane filtration system to concentrate the extract to obtain algal pulp; the membrane molecular weight cut-off is les...

Embodiment 3

[0035] The extraction method of extracting phycocyanin from spirulina, the processing steps of the preparation method are:

[0036] A. Dissolve fresh spirulina in sterilized deionized water at a ratio of 1:20, and freeze and thaw repeatedly three times;

[0037] B. Add the spirulina liquid to the high-pressure homogenizer, and homogenize it 3 times under the state of 30Kpa;

[0038] C. Add the homogenized spirulina liquid to the ultrasonic breaker and use ultrasonic waves to break the algae cell wall to dissolve the phycobiliprotein; extract the feed liquid;

[0039] D. Centrifuge the extracted feed liquid treated by the wall in a butterfly centrifuge for 30 minutes to remove cell debris, and the water phase is phycocyanin extract; the centrifugal force of the centrifuge is 5000×g.

[0040] E. The phycocyanin extract enters the tubular ultrafiltration membrane filtration system to concentrate the extract to obtain algal pulp; the membrane molecular weight cut-off is less than...

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Abstract

The invention discloses a method for extracting phycocyanobilin from spirulina. The method comprises the following process steps: A, dissolving the fresh spirulina in sterile deionized water in the ratio of 1:(10-30) and repeatedly freezing and thawing for three times; B, adding the spirulina liquid to a high-pressure homogenizer and circularly homogenizing for 2-3 times in the state of 20-40Kpa; C, adding the homogenized spirulina liquid to an ultrasonic crusher and crushing the cell walls of the spirulina by use of ultrasonic waves to dissolve out the phycobiliprotein, and extracting the liquid; D, centrifuging the wall-crushed extract for 20-40 minutes, removing cell debris, and obtaining thewater phase as the phycocyanobilin extract; E, concentrating the phycocyanobilin extract in a tubular ultrafiltration membrane filter system to obtain spirulina pulp, wherein the molecular cutoff of the membrane is smaller than 150000 Da and the concentration multiple is 10-15; and F, performing spray-drying on the spirulina pulp under the condition that the temperature is 180 DEG C, thereby obtaining a phycocyanobilin dry powder product.

Description

technical field [0001] The invention relates to the technical field of biological extraction, in particular to an extraction method for extracting phycocyanin from spirulina. Background technique [0002] Phycocyanin can improve the activity of the lymphatic system, enhance the body's immunity, resist tumors, promote the proliferation of hematopoietic cells and bone marrow cells, and significantly reduce the damage of radiation to peripheral blood cells and bone marrow cells in mice. It has good health care functions and auxiliary curative effects. Spirulina soluble polysaccharides have the functions of improving the body's immunity, anti-radiation and anti-cancer. In addition to being used as a medicine, phycocyanin, as a water-soluble blue pigment, has a wide range of applications and is an additive for various high-end foods. It not only has bright colors, but also has health care effects. [0003] In recent years, my country's spirulina factories have developed rapidly....

Claims

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Application Information

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IPC IPC(8): C07K14/795C07K1/34
CPCC07K14/795
Inventor 郑祖凤郑训艺
Owner JIANGXI ZHONGZAO BIOTECH
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