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Marine zobellella sp. and application thereof

A Zobelella and marine technology, applied in the field of microbial applications, can solve the problems of low removal efficiency of high-concentration nitrogen-containing wastewater, etc., achieve high nitrogen removal efficiency, strong tolerance and removal ability, and increase practicality performance effect

Active Publication Date: 2015-08-19
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many patents and literature on aerobic denitrifying bacteria, especially less from marine sources, and the reported strains have low removal efficiency for high-concentration nitrogen-containing wastewater

Method used

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  • Marine zobellella sp. and application thereof
  • Marine zobellella sp. and application thereof
  • Marine zobellella sp. and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Enrichment, isolation and identification of Zobellella sp. CYT1-8

[0042] 1. Enrichment and isolation of bacterial strains: Take 10 g of sediment samples from Zhangzhou vannamei mariculture pond, add 90 mL of enrichment medium (sodium acetate 1 L, sodium citrate 2.5 L, NaCl 30 L, KH 2 PO 4 2L,Na 2 HPO 4 3L, MgSO 4 ·7H 2 O0.05L, FeSO 4 ·7H 2 O 0.02L, CaCl 2 0.02L, distilled water 1000mL, pH 7.6~8.2, sterilized at 121℃ for 20min), with 0.5L NO 2 - -N as the only nitrogen source, 28 ℃, 160rpm / min shaking culture, the first enrichment of aerobic denitrifying bacteria. After 15 days, take 10mL sample solution from the initial enrichment culture medium, transfer it to fresh enrichment culture medium, and add 1.0L NO 2 - -N as the only nitrogen source, 28°C, 160rpm / min shaking culture, for the second enrichment. After 15 days, take 10mL sample solution from the secondary enrichment medium, transfer it to fresh enrichment medium, add 1.5L NO 2 - -N a...

Embodiment 2

[0049] Example 2 Optimization of denitrification conditions for Zobellella sp. CYT1-8

[0050] Scrape fresh bacteria from the MA plate, resuspend with sterile sea water, and adjust the bacterial concentration to 10 9 cfu / mL, according to the inoculation amount of 0.2% to inoculate to contain a certain concentration of NO 3 - -N or NO 2 - In the basal medium of -N, the denitrification conditions of the strains were measured at different carbon sources, pH values, temperatures, salinities, and C / N ratios, and the denitrification conditions were optimized.

[0051] Basal medium: KH 2 PO 4 2L,Na 2 HPO 4 3L, MgSO 4 ·7H 2 O 0.05L, FeSO 4 ·7H 2 O 0.02L, CaCl 2 0.02L, 1000mL of distilled water, sterilized at 121°C for 20min.

[0052] Experimental determination method: the determination of nitrate nitrogen is determined by the zinc cadmium reduction method of GB T12763;

[0053] 1. Effect of different carbon sources on the denitrification performance of Zobellella sp. ...

Embodiment 3

[0058] Denitrification performance measurement of Zobelella under the optimal conditions of embodiment 3

[0059] Pick a single colony of Zobellella sp. CYT1-8 and streak it onto a Marine Agar solid plate, culture it at 28°C for 24 hours, take it out, scrape the fresh bacteria, and make a bacterial suspension with nitrogen-free medium , the strain concentration was adjusted to 10 9 cfu / mL, according to the inoculation volume of 0.2%, inoculated to 100mL NO 3 - -N or NO 2 - -N is the only nitrogen source in seawater medium (sodium citrate is the only carbon source, C / N=12, pH=7.8, salinity 3%), cultivated in a shaker at 35°C and 160r / min, every Take samples every 4 hours to detect the concentration of nitrite and nitrate, and the concentration of bacteria OD 600 . see results Figure 6 and 7 , the results showed that Zobellella sp. CYT1-8 had high removal efficiency for nitrate and nitrite under optimal conditions, and the denitrification rate reached 100% in 16 hours, ...

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Abstract

The invention discloses marine zobellella sp. and an application thereof, and belongs to the technical field of microbe application. According to the marine zobellella sp. CYT1-8, the preservation number is CCTCC NO:M 2015267; the marine zobellella sp. CYT1-8 can be applied to treatment of nitrite wastewater, denitrification of seawater and freshwater aquaculture organisms, nitrite poison removal, and preparation of aerobic and anaerobic denitrification denitrifying live bacteria preparations in any form; and denitrification of the seawater and freshwater aquaculture organisms comprises denitrification of seawater aquaculture wastewater or freshwater aquaculture wastewater, but not limited to denitrification of seawater aquaculture wastewater or freshwater aquaculture wastewater.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to a strain of marine Zobelella and its application. Background technique [0002] In natural water bodies, nitrogen exists in nine different valence forms from -3 to +5. Under the joint action of biotic and abiotic factors, they are continuously transferred and transformed in the water body, forming a complex dynamic cycle. Among them, nitrite is an important intermediate product in this cycle process, and it is also a potentially toxic inorganic nitrogen compound. As an intermediate product of nitrogen transformation in water, nitrite is produced and eliminated mainly in two reactions of nitrification and denitrification. Under normal circumstances, the nitrite content in the water body is very low and will not affect the normal growth of farmed animals. However, due to the intensive management and high-density farming of the modern commercial farmi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16
CPCC02F3/34C02F2101/16C12N1/205C12R2001/01
Inventor 邵宗泽王丽萍李小义
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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