Method for preparing concentrated fish oil fatty acid glyceride

A technology of fatty acid glyceride and fatty acid ethyl ester, which is applied in the production and fermentation of bulk chemicals, can solve the problems of high cost, long synthesis route, low DHA and EPA purity, etc., and achieves the effect of reducing cost and simplifying process steps.

Inactive Publication Date: 2015-08-19
SHENZHEN HAIYOUKANG BIOTECH CO LTD
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AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing concentrated fish oil fatty acid glycerides, aiming to solve the problems of low purity, long synthetic route and high cost of DHA and EPA extracted from fish oil in the prior art

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  • Method for preparing concentrated fish oil fatty acid glyceride

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Embodiment 1

[0041] A method for preparing concentrated fish oil fatty acid glycerides, the process steps are as follows figure 1 As shown, including the following steps:

[0042] S11. Use enzymatic hydrolysis technology to prepare deep-sea fish crude fish oil: deep-sea fish is crushed by a bone crusher to form a minced meat, weigh 600g of meat slurry, add water to seal at the ratio of meat:water=0.5:1 (m:m), and place it in a dark room , Under the condition of nitrogen gas, cook at 75℃ for 1h. Use NaOH to adjust the pH to 8.0. Add 2% trypsin (trypsin: food grade, Zhengzhou Hongcheng Chemical Products Co., Ltd.), and enzymolyze it in a dark room at 20°C for 17 hours. After centrifugation at 3000r / min for 20 minutes, take the upper enzymatic hydrolysate, which is crude fish oil;

[0043] S12. Preparation of refined fish oil: add 0.5% phosphoric acid (phosphoric acid: crude fish oil) by volume to the crude fish oil prepared in step S11 for degumming, the concentration of phosphoric acid is 70%...

Embodiment 2

[0048] A method for preparing concentrated fish oil fatty acid glycerides includes the following steps:

[0049] S21. Preparation of crude deep-sea fish oil using enzymatic hydrolysis technology: refer to step 11 of Example 1, wherein the cooking temperature is 85°C, the enzymatic hydrolysis temperature is 25°C, and the centrifugal speed is 4000r / min;

[0050] S22. Preparation of refined fish oil: refer to step 12 of Example 1; wherein the volume ratio of phosphoric acid: crude fish oil is 1.5%, the concentration of phosphoric acid is 75%; the volume ratio of KOH: crude fish oil is 3%, and the concentration of KOH is 30% , The centrifugal speed is 4000r / min;

[0051] S23. Esterification of refined fish oil to prepare fatty acid ethyl esters: refer to step 13 of Example 1; wherein the mass ratio of potassium ethoxide: refined fish oil is 1.0%, the mass ratio of ethanol: refined fish oil is 2.0, and the heat treatment is 60°C water bath heating In 3 hours, acetic acid was added and th...

Embodiment 3

[0055] A method for preparing concentrated fish oil fatty acid glycerides includes the following steps:

[0056] S31. Preparation of crude deep-sea fish oil using enzymatic hydrolysis technology: refer to step 11 of Example 1, wherein the cooking temperature is 90°C, the enzymatic hydrolysis temperature is 35°C, and the centrifugal speed is 5000r / min;

[0057] S32. Preparation of refined fish oil: refer to step 12 of Example 1; wherein the volume ratio of phosphoric acid: crude fish oil is 3%, the concentration of phosphoric acid is 85%; the volume ratio of NaOH: crude fish oil is 6%, and the concentration of NaOH is 50% , The centrifugal speed is 5000r / min;

[0058] S33. Esterification of refined fish oil to prepare fatty acid ethyl esters: refer to step 13 of Example 1; wherein the mass ratio of potassium ethoxide: refined fish oil is 1.5%, the mass ratio of ethanol: refined fish oil is 4.0, and the heat treatment is 80°C water bath heating In 5 hours, citric acid is added and the...

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Abstract

The present invention belongs to the technical field of biological pharmacy, and provides a method for preparing concentrated fish oil fatty acid glyceride. The method comprises: preparing fatty acid ethyl ester by using refined fish oil as a raw material, carrying out a supercritical fluid extraction treatment on the fatty acid ethyl ester, concentrating, and carrying out an enzyme reaction treatment by using lipase. With the method of the present invention, EPA and DHA in natural fish oil can be effectively converted into the unsaturated fatty acid ethyl esters while the saturated fatty acid ethyl ester type fish oil is converted into the unsaturated fatty acid glyceride so as to be concentrated, such that the EPA content and the DHA content in the concentrated fish oil fatty acid glyceride can be effectively improved, the process steps are simplified, and the cost is reduced.

Description

Technical field [0001] The invention belongs to the technical field of medical functional health products, and particularly relates to a method for preparing concentrated fish oil fatty acid glycerides. Background technique [0002] Deep-sea fish oil is rich in a variety of essential polyunsaturated fatty acids. It is the only commercial source of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which can effectively prevent cardiovascular and cerebrovascular diseases. Promote the development of the retina and delay the aging of the brain. It also has good effects in anti-inflammatory and anti-allergic aspects. Among them, DHA and EPA have physiological functions such as lowering blood lipids, preventing and treating cardiovascular and cerebrovascular diseases, enhancing the function of the nervous system, strengthening the brain, enhancing immunity, inhibiting tumor growth, and protecting eyesight. As people pay more and more attention to deep-sea fish oil, the medic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/6472Y02P20/54
Inventor 陈丽娟王践云钟舟窦庶华
Owner SHENZHEN HAIYOUKANG BIOTECH CO LTD
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