Industrial-grade preparative liquid chromatographic separation method of mogroside V

A technology for the preparation of mogrosides and liquid phases, which is applied in chemical instruments and methods, glycoside steroids, steroids, etc., and can solve problems that affect the use efficiency and lifespan of liquid phase fillers, are not suitable for industrial production, and have no production scale-up. , to achieve the effect of stable and reliable product quality, high degree of automation, and pollution prevention

Active Publication Date: 2015-09-09
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is not suitable for industrial production due to the complicated process, low yield and high cost due to repeated extraction and chromatography.
[0007] CN104086614A discloses a preparation method of Luo Han Guo extract suitable for industrial production. The method is to crush fresh Luo Han Guo, extract, concentrate, centrifuge, ultrafiltration, multi-stage adsorption and separation of macroporous resin, and perform nanofiltration, concentration and drying. Finally, four products with different specifications can be produced at the same time, and the total yield of mogroside V is >70%. This method is suitable for industrial production, but the purity of the obtained product is low, and t

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Dissolving and filtering: take 150g of the crude product of Momordica grosvenori (Mogroside V content is 25%), dissolve it with 2500mL of methanol, filter with 200 mesh stainless steel mesh, and obtain the filtrate;

[0036] (2) Centrifugation and ultrafiltration: The filtrate obtained in step (1) is subjected to tubular centrifugation at a centrifugation rate of 14000r / min, and then a roll-type ultrafiltration membrane is used for secondary ultrafiltration. The molecular weight cut-off of the ultrafiltration membrane is 30,000 respectively. Dalton and 4000 Dalton, get 2250mL ultrafiltrate;

[0037] (3) Preparative liquid chromatography separation: the ultrafiltrate obtained in step (2) is injected into the dynamic axial compression preparative liquid chromatography system through the injection pump, the injection volume is 2250 mL, advanced pre-column processing, and the pre-column specification is Ф200×50mm, the packing is octadecyl reversed-phase silica gel, the parti...

Embodiment 2

[0045] (1) Dissolution and filtration: take 150g of the crude product of Momordica grosvenori (Mogroside V content is 25%), dissolve it with 2300 mL of methanol, filter with 200 mesh stainless steel mesh, and obtain the filtrate;

[0046] (2) Centrifugation and ultrafiltration: the filtrate obtained in step (1) is subjected to tubular centrifugation at a centrifugation rate of 15000r / min, and then a roll-type ultrafiltration membrane is used for secondary ultrafiltration. The molecular weight cut-off of the ultrafiltration membrane is 50,000 respectively. Dalton and 6000 Dalton, get 2125mL ultrafiltrate;

[0047] (3) Preparative liquid chromatography separation: the ultrafiltrate obtained in step (2) is injected into the dynamic axial compression preparative liquid chromatography system through the injection pump, the injection volume is 2125 mL, advanced pre-column processing, and the pre-column specification Ф200 ×50mm, the packing is octadecyl reversed-phase silica gel, the part...

Embodiment 3

[0055] (1) Dissolve and filter: take 150g of the crude product of Momordica grosvenori (Mogroside V content is 25%), dissolve it with 1,850 mL of methanol, filter with 200 mesh stainless steel mesh, and obtain the filtrate;

[0056] (2) Centrifugation and ultrafiltration: the filtrate obtained in step (1) is subjected to tubular centrifugation at a centrifugation rate of 12000r / min, and then a roll-type ultrafiltration membrane is used for secondary ultrafiltration. The molecular weight cut-off of the ultrafiltration membrane is 40,000 respectively. Dalton and 5000 Dalton, get ultrafiltrate 1715mL;

[0057] (3) Preparative liquid chromatography separation: The ultrafiltrate obtained in step (2) is injected into the dynamic axial compression preparative liquid chromatography system through the injection pump, the injection volume is 1715 mL, advanced pre-column processing, and the pre-column specification is Ф200 ×50mm, the packing is octadecyl reversed-phase silica gel, the particl...

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Abstract

An industrial-grade preparative liquid chromatographic separation method of mogroside V comprises steps as follows: (1) dissolution and filtration: adding methanol to a crude Siratia grosvenorii product in the solid-to-liquid ratio of 1:(10-20) for dissolution, and performing filtration to obtain a filtrate; (2) centrifugation and ultrafiltration: centrifuging the filtrate obtained in Step (1), and then performing ultrafiltration to obtain an ultrafiltrate; (3) preparative liquid chromatographic separation: injecting the ultrafiltrate obtained in Sep (2) into a dynamic axial compression preparative liquid chromatographic system, then performing elution with an eluent, and collecting a target-section eluent; (4) concentration and drying: concentrating the target-section eluent obtained in Step (3), and then performing drying to obtain the mogroside V. The method is simple in technological process, high in automation degree, high in controllability and high in refining degree, the product quality reproducibility is good, and the product quality is stable and reliable; the purity of the mogroside V separated with the method is higher than or equal to 95%, and the yield is higher than or equal to 75%.

Description

technical field [0001] The invention relates to a separation method of mogroside V, in particular to an industrial-grade liquid chromatography separation method of mogroside V. Background technique [0002] Luo Han Guo, Latin scientific name Siratia grosvenorii, alias La Han Guo, fake bitter gourd, is native to Guilin, Guangxi, and Yongfu County and Longsheng County are rich in it, and its quality is high. Currently, it is also planted in Hunan, Jiangxi, Guangdong and other provinces. [0003] Luo Han Guo is a plant with the same origin of medicine and food. It can be used to make drinks and medicine. It is rich in nutrition and extremely sweet. Studies have shown that the main sweet substances in Luo Han Guo are mogrosides, and the most important chemical component is mogroside V, which has a sweetness of more than 250 times that of sucrose, and its calories are only 20 times that of sucrose. one. [0004] Mogroside V is a natural sweetener, which has the characteristics ...

Claims

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Application Information

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IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 唐方华黄华学
Owner HUNAN HUACHENG BIOTECH
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