Industrial-grade preparative liquid chromatographic separation method of mogroside V

Abstract

An industrial-grade preparative liquid chromatographic separation method of mogroside V comprises steps as follows: (1) dissolution and filtration: adding methanol to a crude Siratia grosvenorii product in the solid-to-liquid ratio of 1:(10-20) for dissolution, and performing filtration to obtain a filtrate; (2) centrifugation and ultrafiltration: centrifuging the filtrate obtained in Step (1), and then performing ultrafiltration to obtain an ultrafiltrate; (3) preparative liquid chromatographic separation: injecting the ultrafiltrate obtained in Sep (2) into a dynamic axial compression preparative liquid chromatographic system, then performing elution with an eluent, and collecting a target-section eluent; (4) concentration and drying: concentrating the target-section eluent obtained in Step (3), and then performing drying to obtain the mogroside V. The method is simple in technological process, high in automation degree, high in controllability and high in refining degree, the product quality reproducibility is good, and the product quality is stable and reliable; the purity of the mogroside V separated with the method is higher than or equal to 95%, and the yield is higher than or equal to 75%.

Description

technical field [0001] The invention relates to a separation method of mogroside V, in particular to an industrial-grade liquid chromatography separation method of mogroside V. Background technique [0002] Luo Han Guo, Latin scientific name Siratia grosvenorii, alias La Han Guo, fake bitter gourd, is native to Guilin, Guangxi, and Yongfu County and Longsheng County are rich in it, and its quality is high. Currently, it is also planted in Hunan, Jiangxi, Guangdong and other provinces. [0003] Luo Han Guo is a plant with the same origin of medicine and food. It can be used to make drinks and medicine. It is rich in nutrition and extremely sweet. Studies have shown that the main sweet substances in Luo Han Guo are mogrosides, and the most important chemical component is mogroside V, which has a sweetness of more than 250 times that of sucrose, and its calories are only 20 times that of sucrose. one. [0004] Mogroside V is a natural sweetener, which has the characteristics ...

AI Technical Summary

Problems solved by technology

This method is not suitable for industrial production due to the complicated process, low yield and high cost due to repeated extraction and chromatography.

[0007] CN104086614A discloses a preparation method of Luo Han Guo extract suitable for industrial production. The method is to crush fresh Luo Han Guo, extract, concentrate, centrifuge, ultrafiltration, multi-stage adsorption and separation of macroporous resin, and perform nanofiltration, concentration and drying. Finally, four products with different specifications can be produced at the same time, and the total yield of mogroside V is >70%. This method is suitable for industrial production, but the purity of the obtained product is low, and the subsequent treatment process is relatively complicated

[0009] CN104530168A discloses an industrialized preparation method of Mogroside V. The method is to extract and filter the Luo Han Guo, use a preparative liquid chromatography system to separate, concentrate and dry the product, and the product purity can reach more than 95%. The process of the method is simple. The product has high purity, but because it has not been systematically filtered and purified before being put on the column, it seriously affects the service efficiency and life of the prepared liquid phase packing, the production cost is high, and it is still in the test scale-up stage, and there is no example of production scale-up