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A method for rapidly cultivating Pseudomonas aeruginosa and its special medium

A Pseudomonas aeruginosa and culture medium technology, applied in the biological field, to achieve the effects of promoting growth, long stable period, and shortening culture time

Inactive Publication Date: 2017-11-17
刘慧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the diagnosis of the disease, there is a large lag

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1 preparation culture medium

[0011] (1) Rapid culture medium: phenylacetic acid 1.2g, casein peptone 5g, magnesium sulfate 0.08g, sodium chloride 2g, dipotassium hydrogen phosphate 2.5g, zinc sulfate 1mg, glucose 1g, pyridoxine hydrochloride 0.5mg, egg Phospholipid 1g, tetrachlorotetraiodofluorescein sodium 0.001g, glycerol 1ml, pH8.0, add distilled water to 1000ml. Sterilize to obtain the culture medium.

[0012] (2) culture medium commonly used in prior art:

[0013] Formula (per liter): Casein peptone 17g, soybean peptone 3g, sodium chloride 5g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, lecithin 1g, Tween 80 7g, final pH 7.2±0.2, sterilized to obtain the the above-mentioned culture medium.

[0014] (3) Common beef extract peptone medium.

Embodiment 2

[0015] Embodiment 2 colony culture experiment

[0016] Press 2×10 6 The inoculum amount per ml was respectively inoculated into Pseudomonas aeruginosa, Escherichia coli, Salmonella, and Staphylococcus aureus in three kinds of mediums, and placed at 37 degrees Celsius to cultivate for 6 hours, and the number of bacteria was measured with a hemocytometer counting method. The experimental results are as follows:

[0017] Bacterial concentration

[0018] From the above data, it can be shown that the rapid medium provided by the application can have the characteristics of promoting the rapid proliferation of Pseudomonas aeruginosa preferably, and this medium is not suitable for the reproduction of other common bacteria, which is conducive to the purification of Pseudomonas aeruginosa nourish.

Embodiment 3

[0019] Optimization of Pseudomonas aeruginosa culture conditions in embodiment 3 medical samples

[0020] Take a little secretion from the medical sample, add it to a test tube containing 3mL of rapid culture medium, first incubate at 220r / min at 42°C for 2h, then incubate at 220r / min at 37°C for 6h, the color of the culture solution turns greenish. Indicates the presence of Pseudomonas aeruginosa in medical samples. The conventional PCR identification in the field also showed that the bacteria in the culture solution were Pseudomonas aeruginosa.

[0021] It can be found from Example 3 that compared with the conventional culture method in the prior art, the culture method shortens the time for culture and identification on a large scale, and saves a lot of valuable time for the inspection of germs. It has very good application value.

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PUM

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Abstract

The invention discloses a culture medium capable of promoting the rapid proliferation of pseudomonas aeruginosa. The culture medium disclosed by the invention has a better cultivation effect and is prepared from multiple components according to researches for hundreds of times. According to the culture medium capable of promoting the rapid proliferation of the pseudomonas aeruginosa, the cell viability can be remarkably improved. Compared with a conventional culture medium, the culture medium capable of promoting the rapid proliferation of the pseudomonas aeruginosa has the advantages that the effects of promoting the growth of bacteria and shortening the cultivation time are obvious.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for rapidly cultivating Pseudomonas aeruginosa and a special culture medium thereof. Background technique [0002] Pseudomonas aeruginosa, also known as Pseudomonas aeruginosa (scientific name Pseudomonas aeruginos), is a Gram-negative, aerobic, rod-shaped bacterium with only one-way motility. Pseudomonas aeruginosa is an opportunistic pathogen with strong adaptability to the external environment and can survive for a long time in a humid environment. Pseudomonas aeruginosa exists in normal human skin, especially in moist parts (such as armpits, perineum, ear canal, respiratory tract and intestinal tract, etc.), and intact skin and mucous membranes can effectively resist Pseudomonas aeruginosa as a barrier However, the normal defense mechanism of the human body is reduced or damaged (such as skin and mucous membrane damage, agranulocytosis, hypoproteinemia, radiotherapy and chemo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/385
CPCC12N1/20
Inventor 刘慧杨宗军张璐
Owner 刘慧