Method for preparing bean flour and functional soybean protein rich in glyceollin by adopting solid fermentation method

A technology of soybean antitoxin and solid fermentation method, which is applied in the field of food processing, and achieves the effect of mild process conditions and good safety

Inactive Publication Date: 2015-09-16
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The anti-tumor, cardiovascular disease-improving and osteoporosis-preventing activities of soybeans rich in aglycones and Glyceollins are related to the polyphenol-protein interactions in soybeans, and there is no Glyceollins and protein interactions Study of interacting protein products

Method used

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  • Method for preparing bean flour and functional soybean protein rich in glyceollin by adopting solid fermentation method
  • Method for preparing bean flour and functional soybean protein rich in glyceollin by adopting solid fermentation method
  • Method for preparing bean flour and functional soybean protein rich in glyceollin by adopting solid fermentation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Undamaged group: take 6g of soybean seeds, soak them with 70% alcohol for 2 minutes, then soak them with sterile water for 3 times, and finally soak them with sterile water at 25°C for 12 hours, and peel them off. With 1.5mL spore suspension (Aspergillus oryzae spore number is 1×10 7 pcs) into soybeans and mix well with a sterile spoon. Place the infected soybeans in petri dishes, 25-30 cloves per dish, seal with a parafilm, culture in a dark box at 25°C, and freeze-dry the fermented soybeans for later use.

[0029] Injury group: Soybean seeds were taken, soaked with 70% alcohol for 2 minutes, then soaked with sterile water for 3 times, and finally soaked in sterile water at 25°C for 12 hours, peeled, and scored 3 times on the surface of the beans with a scalpel Damage treatment.

[0030] With 1.5mL spore suspension (Aspergillus oryzae spore number is 1×10 7 ) into the damaged soybeans, and mix evenly with a sterile spoon. Put 6 g of soybeans that have been infected...

Embodiment 2

[0036] Take 6g of soybean seeds, soak them in alcohol with a mass concentration of 70% for 2 minutes, then soak them in sterile water for 3 times, and finally soak them in sterile water at 25°C for 12 hours, peel and damage them. With 1.5mL spore suspension (Aspergillus oryzae spore number is 1×10 7 pcs) into the damaged soybeans and mix well with a sterile spoon. Place the contaminated soybeans in petri dishes, 25-30 petri dishes per dish, seal with a parafilm, and culture in a dark box at 25°C for 0d, 1d, 3d, 5d, respectively, and the obtained fermented soybeans are freeze-dried, crushed, and set aside. Non-inoculated soybeans (0) were used as blank control. The test method is the same as in Example 1, and the test results are shown in Table 2.

[0037] Aglycone content (mg / kg) and Glys content (mg DE / kg DW) change in the embodiment 2 of table 2

[0038]

[0039] After calculation, as can be known from the results in Example 2, taking soybean as raw material, when the ...

Embodiment 3

[0041] Take 6g of soybean seeds, soak them with 70% alcohol for 2 minutes, then soak them with sterile water for 3 times, and finally soak them with sterile water at 25°C for 12 hours, peel and damage them. Inoculate 10 spores per gram of beans 7 a, 10 8 and 10 9 The spore suspension (1.5mL) was added to the damaged soybean and mixed with a sterile spoon. Put the contaminated soybeans in petri dishes, 25-30 petri dishes per dish, seal with parafilm, and culture in a dark box at 25°C for 3 days respectively. Non-inoculated soybeans (0) were used as blank control. The test method is the same as in Example 1, and the test results are shown in Table 3.

[0042] Aglycone content (mg / kg) and Glys content (mg DE / kg DW) change in the embodiment 3 of table 3

[0043]

[0044] Using high-isoflavone soybeans as raw materials, when the conditions such as the cultivation time are constant, the number of spores is 10 7 、10 8 、10 9 The bacteria inoculated into the treated soybeans...

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Abstract

The invention discloses a method for preparing bean flour and functional soybean protein rich in glyceollin by adopting a solid fermentation method. The method comprises the following steps: inoculating an aspergillus oryzae spore suspension into peeled and damaged soybeans which are soaked for 6-12 hours to obtain contaminated soybeans; putting the contaminated soybeans into a culture dish, and sealing with a sealing film; carrying out solid fermentation in a dark box at 20-30 DEG C for 3-5 days, and carrying out freeze-drying and crushing to obtain the bean flour rich in glyceollin; degreasing the obtained bean flour rich in the glyceollin with normal hexane; and carrying out alkali-solution and acid-isolation on the obtained degreased bean flour, thus obtaining the functional soybean protein rich in the glyceollin. According to the method disclosed by the invention, the bean flour and the protein reach the effect of gathering isoflavone aglycones and Glyceollins; the protein is relatively high in yield; and the bean flour and the functional soybean protein are suitable for industrial production, and can be applied to flour products, dairy products and vegetable protein drinks for menopause women as special protein ingredients.

Description

technical field [0001] The invention relates to soybean powder and soybean protein, in particular to a method for preparing soybean powder and protein rich in soybean antitoxin by a solid fermentation method; it belongs to the technical field of food processing. Background technique [0002] my country's per capita resources are quite scarce, and the contradiction between the huge population and material needs and the limited carrying capacity and vulnerability of the ecological environment is becoming increasingly acute, and the Chinese population is rapidly aging. Population aging and unreasonable diet may lead to high incidence of senile osteoporosis, diabetes and other related diseases, especially for menopausal women. Due to the decline in the secretion of estrogen, menopausal and postmenopausal women are prone to other diseases, such as breast cancer and menopausal depression. Therefore, for middle-aged and elderly people, especially menopausal and postmenopausal wome...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/20A23L1/29A23L1/305A23J1/14
CPCA23J1/148A23V2002/00A23V2250/5488
Inventor 王金梅陈燕琼杨晓泉胡文锋苏铧佳颜喆
Owner SOUTH CHINA UNIV OF TECH
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