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Technological method for efficiently extracting ginkgo flavone

A technology of ginkgo flavonoids and process methods, applied in the biological field, can solve the problems of low content of water-soluble ginkgo flavonoids, high extraction rate of total flavonoids, large amount of solvent, etc., and achieve the effects of reducing dosage, increasing extraction rate, and improving extraction efficiency

Inactive Publication Date: 2015-09-16
江苏恒天生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to solve the problems existing in the existing ginkgo flavonoids extraction by solvent method, overcome the disadvantages of large amount of solvent, high residue, low extraction rate, low content of water-soluble ginkgo flavonoids, etc., and provide a process for efficiently extracting ginkgo flavonoids , this method uses less solvent and has a high extraction rate of total flavonoids, which is suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1) Superfinely pulverize ginkgo leaves into 1000 mesh ginkgo leaf powder;

[0020] 2) Weigh 1 kg of ultrafinely pulverized Ginkgo biloba powder, mix it with water at a mass ratio of 1:15, heat to 80°C for 60 minutes to inactivate endogenous enzymes;

[0021] 3) Adjust the temperature to 45°C, adjust the pH value to 4.2, and then add a compound enzyme with 0.1% mass of ginkgo leaf powder. The compound enzyme is composed of cellulase, β-glucanase and pectinase in a ratio of 2:1:1. Specific composition, enzymatic hydrolysis for 10 hours, heating to 100°C, and solid-liquid separation to obtain enzymatic hydrolysis solution;

[0022] 4) Adjust the pH value of the above enzymatic solution to 5.5, add absolute ethanol to make the concentration reach 30% V / V, stir well, then add glucosidase to make the activity reach 0.1AGU / L, and discard after 24 hours of reaction Impurities are removed to obtain an extract;

[0023] 5) Recover ethanol from the extract, heat and concentrate ...

Embodiment 2

[0028] 1) Superfinely pulverize ginkgo leaves into 2000 mesh ginkgo leaf powder;

[0029] 2) Weigh 5kg of ultrafinely pulverized ginkgo leaf powder, mix it with water at a mass ratio of 1:20, heat to 100°C for 40 minutes to inactivate endogenous enzymes;

[0030] 3) Adjust the temperature to 50°C, adjust the pH value to 4.5, and then add 0.2% compound enzyme of ginkgo leaf powder. Specific composition, enzymatic hydrolysis for 12 hours, heating to 100°C, and solid-liquid separation to obtain enzymatic hydrolysis solution;

[0031] 4) Adjust the pH value of the above enzymatic hydrolysis solution to 5.0, add absolute ethanol to make the concentration reach 40% V / V, stir well and then add glucosidase to make the activity reach 0.2AGU / L, and discard after 20 hours of reaction Impurities are removed to obtain an extract;

[0032] 5) Recover ethanol from the extract, heat and concentrate to 1 / 4 of the original volume to obtain a concentrate;

[0033] 6) Pour the concentrated sol...

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PUM

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Abstract

The invention relates to a technological method for efficiently extracting ginkgo flavone from gingkoes by an ultramicro-pulverization auxiliary biological enzyme method. The technological method comprises the steps that (1) folium ginkgoes are subjected to ultramicro-pulverization to be 500-3000 meshes; (2) water with the weight being three to five times that of materials is added, and 0.01%-0.2% of compound enzymes are added to conduct enzymatic extraction for 2-12 h; (3) the pH value is regulated to be 5.5-6.5, a certain amount of absolute ethyl alcohol and glucosaccharase are added to react for 12-24 h; (4) the extracting solution is concentrated to recycle ethanol; (5) the concentrated solution is subjected to macroporous resin absorption, and ethanol elution is conducted; (6) the elution solution is decompressed and condensed through the recycled ethanol and then is subjected to spray drying, and folium ginkgo flavone compound products are obtained. The technological method is a novel ginkgo flavones extracting technology, the usage amount of solvents in the extracting process can be reduced, the flavone extraction efficiency is improved, and the content of water-soluble ginkgo flavones is improved.

Description

technical field [0001] The invention relates to a technology for extracting active ingredients in natural products, belonging to the field of biotechnology. Specifically relates to a process method for efficiently extracting ginkgo flavonoids. Background technique [0002] Ginkgo biloba (Ginkgo biloba L.) is a relic plant that survived on the earth during the glacial period, and is known as a "living fossil". Ginkgo biloba can not only be used as a famous garden ornamental tree species, but also has high economic value and nutritional value because it is rich in various physiologically active substances. Ginkgo biloba extract (EGB) has unique pharmacological activity and huge clinical application value, and its application fields include medicine, food, health care products, cosmetics, handicrafts and plant protection. A large number of studies have shown that flavonoids are one of the main physiologically active components in Ginkgo biloba extract (EGB), which has pharmac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16A61P9/08A61P7/02A61P3/06A61P35/00A61K127/00
Inventor 周彬程耀波王荣彬
Owner 江苏恒天生物科技有限公司
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