Method for manufacturing artificial blood vessels with double-layered structures and application of artificial blood vessels

A technology of artificial blood vessels and double-layer structure, which is applied in the direction of human tubular structure devices, blood vessels, veterinary instruments, etc., can solve the problems of hindering the regeneration of pseudo-natural blood vessels, the inability to regenerate smooth muscle layers, and the lack of orientation of smooth muscle layers. Blood compatibility, safe and non-toxic materials, and the effect of avoiding aneurysm

Active Publication Date: 2015-09-23
领博生物科技(杭州)有限公司
View PDF9 Cites 29 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of this method is that the graft contains vascular component cells, which have some functions of the corresponding cells, and has better compatibility with the implantation site; however, this method has some disadvantages: (1) the patient's own cells are required; ( 2) It takes a long time for in vitro culture; (3) In vitro culture causes cells to easily lose their original functions; (4) The product cannot be obtained in time, the cost of treatment is high, and there is a risk of infection, etc.
Traditional small-caliber tissue engineered vascular scaffolds mostly adopt random porous structures. After implantation in the body, host cells grow randomly in the scaffolds, making it impossible to regenerate the smooth muscle layer with axial orientation characteristics.
Regeneration of non-oriented smooth muscle layers hinders regeneration of pseudo-native blood vessels

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for manufacturing artificial blood vessels with double-layered structures and application of artificial blood vessels
  • Method for manufacturing artificial blood vessels with double-layered structures and application of artificial blood vessels

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Preparation of PGA oriented microfiber inner layer and PLA random nanofiber outer double-layer structure artificial blood vessel

[0012] Preparation of the inner layer spinning solution: Weigh 1.0 g of PGA with a number average molecular weight of 100,000, add it to 10 ml of hexafluoroisopropanol, stir until it dissolves evenly, and prepare a PGA solution with a concentration fraction of 10% (m / v).

[0013] Preparation of the outer layer spinning solution: Weigh 2.0 g of PLA with a number average molecular weight of 300,000, add it to 10 ml of chloroform, stir until it dissolves evenly, and prepare a solution with a concentration fraction of 20% (m / v) PLA.

[0014] Preparation of the inner layer of the artificial blood vessel: the wet spinning of the inner layer was carried out in a fume hood at room temperature. Connect a cylindrical receiving rod with a diameter of 1.0 mm to a rotating motor. The PGA spinning solution was sucked into the syringe, and the ...

Embodiment 2

[0016] Example 2: Preparation of double-layer structure artificial blood vessel with PLGA oriented microfiber inner layer and PLCL random nanofiber outer layer

[0017] Preparation of the inner layer spinning solution: Weigh 1.2 grams of PLGA (LA / GA, 75:25), add it to 10 ml of chloroform, stir until it dissolves evenly, and prepare a PLGA solution with a concentration fraction of 12% (m / v).

[0018]Preparation of the outer layer spinning solution: Weigh 0.8 g of PLCL (50:50), add it into 10 ml of dichloromethane, stir until it dissolves evenly, and prepare a PLCL solution with a concentration fraction of 8% (m / v).

[0019] Preparation of the inner layer of the artificial blood vessel: the wet spinning of the inner layer was carried out in a fume hood at room temperature. Connect a cylindrical receiving rod with a diameter of 2.0mm to the rotating motor. The 12% PLGA spinning solution was sucked into the syringe, and the needle of the syringe was inserted into the edible oil i...

Embodiment 3

[0021] Example 3: Preparation of artificial blood vessel with double-layer structure of silk fibroin-oriented microfiber inner layer and PCL random nanofiber outer layer

[0022] Preparation of inner layer spinning solution: Weigh 2 grams of silk fibroin freeze-dried product, add to 10mL hexafluoroisopropanol solvent, stir until dissolved evenly, centrifuge at 10000rpm to discard the precipitate, and collect the concentration fraction to be 20% (m / v ) of SF solution.

[0023] Preparation of the outer layer spinning solution: Weigh 1.2 g of PCL and add it to 12 ml of chloroform / methanol (5:1), stir until it dissolves evenly, and obtain a PCL solution with a concentration fraction of 10% (m / v).

[0024] Preparation of the inner layer of the artificial blood vessel: the wet spinning of the inner layer was carried out in a fume hood at room temperature. Connect the 4.0mm diameter cylindrical receiving rod to the rotating motor. The silk fibroin spinning solution is sucked into t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for manufacturing artificial blood vessels with double-layered structures and application of the artificial blood vessels, and discloses a method for manufacturing artificial blood vessels with double-layered structures and a method for applying the artificial blood vessels. The method for manufacturing the artificial blood vessels with the double-layered structures includes a first step, manufacturing inner oriented micron fiber layers of the artificial blood vessels with the double-layered structures; a second step, manufacturing outer random nano-fiber layers of the artificial blood vessels with the double-layered structures. The method for applying the artificial blood vessels includes transplanting the double-layered artificial blood vessels in situ so as to replace lesion blood vessels. The methods have the advantages that vascular smooth muscle cells can be induced in vivo by oriented micron fibers of the inner layers of the artificial blood vessels to be regenerated just like oriented structures of natural blood vessels, sufficient suture strength and mechanical properties of the blood vessels can be guaranteed by the outer random nano-fiber layers, and accordingly the artificial blood vessels can be used for repairing and replacing the lesion natural blood vessels; the double-layered artificial blood vessels have excellent application prospects in the field of medical clinical vascular transplantation.

Description

technical field [0001] The present invention relates to a method for preparing artificial blood vessels, specifically a method for processing artificial blood vessels that can regulate the regeneration of vascular smooth muscle cells in a pseudo-natural orientation in vivo, and is composed of an inner layer of oriented microfibers and an outer layer of random nanofibers, and Therefore, the application of artificial blood vessels in the replacement and repair of damaged blood vessels. Background technique [0002] Cardiovascular disease and peripheral vascular disease caused by atherosclerosis are one of the diseases with the highest morbidity and mortality, which will lead to narrowing of arterial diameter and eventually blockage of blood vessels. At present, there are two main treatment methods for this vascular disease, vasodilator surgery and vascular transplantation, but because vasodilator surgery cannot completely solve the vascular blockage, and it is prone to recurre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/06D04H1/728A61L27/18A61L27/22A61L27/50A61D1/00
Inventor 王连永朱美峰孔德领王志红王恺王淑芳
Owner 领博生物科技(杭州)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap