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Umbilical cord mesenchymal stem cell injection and preparation method and application thereof

A technology of stem cells and injections, applied in the field of biomedicine, can solve problems such as unsatisfactory conditions of ischemic heart disease

Inactive Publication Date: 2015-09-23
北京青藤谷禧干细胞科技研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

TSG6 can promote the repair of heart damage, but a single drug delivery method cannot satisfy complex ischemic heart disease conditions. Therefore, in order to further improve the therapeutic effect, a breakthrough is urgently needed to adopt a new combination of multiple drug delivery methods

Method used

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  • Umbilical cord mesenchymal stem cell injection and preparation method and application thereof
  • Umbilical cord mesenchymal stem cell injection and preparation method and application thereof
  • Umbilical cord mesenchymal stem cell injection and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1 Preparation of umbilical cord mesenchymal stem cells

[0076] In this example, the following steps were used to prepare umbilical cord mesenchymal stem cells:

[0077] (1) Take fresh umbilical cords that have left the mother’s body for less than 2 hours after cesarean section, and check whether the puerpera is carrying infectious disease pathogens (negative hepatitis B surface antigen, hepatitis C antibody, syphilis antibody, and AIDS antibody), and place the fresh umbilical cord containing D- In the special storage bottle of Hanks balance solution, it is transported in a low-temperature ice box;

[0078] (2) The biological safety cabinet was irradiated with ultraviolet rays for 30 minutes, and the water bath shaker was preheated at 37°C in advance, and the umbilical cord sample bottle was placed in the biological safety cabinet for operation. The samples were taken out with long hemostatic forceps and placed in a 150mm sterile glass dish, and the blood on th...

Embodiment 2

[0091] Example 2 Preparation of Umbilical Cord Mesenchymal Stem Cell Injection

[0092] This example uses the following steps to prepare the umbilical cord mesenchymal stem cell injection:

[0093] (1) Prepare 50mL mixed solution, wherein, the volume percentage of electrolyte balance solution is 44%, the mass volume percentage of hydroxyethyl starch 130 / 0.4 is 6%, and the mass volume percentage of adenosine triphosphate disodium-magnesium chloride freeze-dried powder is 10% 30% by volume and 20% human albumin;

[0094] (2) Mix the mixed solution well, filter it with a sterilizing grade hydrophilic filter with a pore size of 0.22 μm, and then slowly add the prepared umbilical cord mesenchymal stem cells into the mixed solution after sterilization, and control the concentration at 5×10 5 -1×10 6 a / mL;

[0095] (3) adding clinical grade dimethyl sulfoxide so that the final volume fraction is 10%;

[0096] (4) Subpackage the prepared umbilical cord mesenchymal stem cell inject...

Embodiment 3

[0098] Example 3 Preparation of Umbilical Cord Mesenchymal Stem Cell Injection

[0099] This example uses the following steps to prepare the umbilical cord mesenchymal stem cell injection:

[0100] (1) prepare 50mL mixed solution, wherein, the volume percentage of electrolyte balance liquid is 25%, the mass volume percentage of hydroxyethyl starch 130 / 0.4 is 5%, the mass volume percentage of adenosine triphosphate disodium-magnesium chloride lyophilized powder is 5% 50% by volume and 20% human albumin;

[0101] (2) Mix the mixed solution well, filter it with a sterilizing grade hydrophilic filter with a pore size of 0.22 μm, and then slowly add the prepared umbilical cord mesenchymal stem cells into the mixed solution after sterilization, and control the concentration at 5×10 5 -1×10 6 a / mL;

[0102] (3) adding clinical grade dimethyl sulfoxide so that the final volume fraction is 20%;

[0103] (4) Subpackage the prepared umbilical cord mesenchymal stem cell injection in ...

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Abstract

The invention relates to umbilical cord mesenchymal stem cell injection and a preparation method and application thereof. The injection comprises umbilical cord mesenchymal stem cells and mixed solution; the mixed solution comprises balanced electrolyte solution, hydroxyethyl starch, adenosine disodium triphosphate-magnesium chloride, dimethyl sulfoxide and human serum albumin. The preparation method comprises the following steps: (1) fully and evenly mixing the balanced electrolyte solution, the hydroxyethyl starch and the human serum albumin, and performing filtration sterilization; (2) adding the umbilical cord mesenchymal stem cells; (3) adding the dimethyl sulfoxide; (4) performing split charging and frozen preservation to finish preparation; the invention also provides the application of the injection in the aspect of drugs for treating chronic ischemic heart diseases. The injection disclosed by the invention has a very good resuscitation effect after the frozen preservation, can be directly injected after being unfrozen and resuscitated, and has a remarkable effect on treatment on the chronic ischemic heart diseases.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an umbilical cord mesenchymal stem cell injection and a preparation method and application thereof. Background technique [0002] Ischemic heart disease (IHD) is myocardial damage caused by an imbalance between coronary blood flow and myocardial cell demands caused by changes in coronary circulation. Despite recent advances in percutaneous coronary intervention (PCI), coronary artery bypass grafting (CABG) and drug therapy, ischemic heart disease remains the number one cause of death worldwide, accounting for 20% of cases Due to severe or diffuse coronary artery disease, conventional drug treatment is not effective and surgical treatment is not possible, so IHD patients are also called "No-option" patients. The clinical treatment of chronic ischemic heart disease is limited, and the curative effect and prognosis are poor, so it is necessary to find new and more effective treatment met...

Claims

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Application Information

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IPC IPC(8): A61K9/08A61K35/28A61K47/42A61K47/36A61K47/26A61K47/20A61K47/12A61P9/10
Inventor 孙磊孙萍宋亚昆马世泽史振生吴东颖
Owner 北京青藤谷禧干细胞科技研究院有限公司
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