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Method for preparing high-gamma-aminobutyric-acid-content tea extract

A technology of tea extract and aminobutyric acid, applied in skin care preparations, plant cells, pharmaceutical formulas, etc., can solve the problems of low content, unfavorable environment, and difficult industrial purification, and achieve high content, short cultivation period, and high yield high rate effect

Active Publication Date: 2015-10-21
PROYA COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the content of γ-aminobutyric acid in fresh tea leaves treated by traditional methods is only 0.15-0.2% of the dry weight of tea leaves, the content is relatively low, and the content of lutein contained in tea leaves is relatively high, industrial purification is more difficult, and a large amount of lutein will be produced. Organic solvent waste liquid, which is not good for the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A. The green tea buds from eighteen fresh Longjing tea trees in Hangzhou West Lake were picked before Qingming Festival, and after being sterilized with 70% ethanol by volume fraction, 10 sections of 2mm long tea buds were cut off with scissors under aseptic conditions. Inoculate in the MS medium of M5519 produced by SIGMA Company, control the room temperature at 25±1°C in dark culture, and cultivate for 7 days to induce tea calli, 4 milky white, 3 khaki, and 3 light green of callus. The cell lines of three colors were respectively subcultured, and the milky white cell line was determined to be a cell line with good growth and high content of γ-aminobutyric acid through the determination of growth rate and γ-aminobutyric acid content, as the following One-step expansion of cultured seed cells;

[0038] B. Preparation of high-yield γ-aminobutyric acid tea stem cell culture medium: weigh 58% MS medium (manufacturer Sigma-Aldrich, model M5519), 34.38% plant gel (manufactu...

Embodiment 2

[0043] A. Picking green tea shoots (the green tea shoots in this example were picked from the green tea trees on Tianmu Mountain in Jiande City before Qingming Festival), after disinfection by volume fraction of 70% ethanol, the tea shoots were cut off 10% with scissors under aseptic conditions. Segments with a length of 2mm were inoculated in the MS medium of M5519 produced by SIGMA Company, cultured in the dark at 25±1°C under controlled room temperature, and cultured for 7 days to induce tea callus, and milky white cell lines were screened as the next step Expand the seed cell of culture, as the seed cell of expansion culture in the next step;

[0044] B. Preparation of high-yield γ-aminobutyric acid tea stem cell culture medium: weigh 80% MS medium (manufacturer Sigma-Aldrich, model M5519), 10% plant gel (manufacturer Sigma-Aldrich, model P8169) by mass ratio , 4.2% NAA (manufacturer Sigma-Aldrich, model N0640), 5.5% sucrose (manufacturer Ferro, purity 99.7%), 0.1% indole ...

Embodiment 3

[0049] A. The green tea buds from eighteen fresh Longjing tea trees in Hangzhou West Lake were picked before Qingming Festival, and after being sterilized with 70% ethanol by volume fraction, 10 sections of 2mm long tea buds were cut off with scissors under aseptic conditions. Inoculate in the MS medium of M5519 produced by SIGMA Company, control the room temperature at 25±1°C and culture in dark, culture for 7 days to induce tea callus, screen the milky white cell line, and use it as the seed cell for the next expansion culture, as The next step is to expand the cultured seed cells;

[0050] B. Preparation of high-yield γ-aminobutyric acid tea stem cell culture medium: weigh 50% MS medium (manufacturer Sigma-Aldrich, model M5519), 38.57% plant gel (manufacturer Sigma-Aldrich, model P8169) according to mass ratio , 7.5% NAA (manufacturer Sigma-Aldrich, model N0640), 3.5% sucrose (manufacturer Ferro, purity 99.7%), 0.2% indole acetic acid (manufacturer Japan TCI, analytically p...

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Abstract

The invention relates to a method for preparing high-gamma-aminobutyric-acid-content tea extract. The method is characterized by including the following steps: A, green-tea sprouts on tea trees are picked and sterilized to be inoculated to MS culture media, and tea calluses are induced; B, a high-gamma-aminobutyric-acid-yield tea stem cell culture solution is prepared; C, the milky tea calluses obtained in the step A are selected as seeds and loaded into the high-gamma-aminobutyric-acid-yield tea stem cell culture solution for suspension cultivation, and solid tea stem cell powder is obtained; D, wall breaking homogenating processing is carried out on the solid tea stem cell powder obtained in the step C; E, a cell wall breaking homogenating mixture obtained in the step D and absolute ethyl alcohol are mixed in the mass ratio 1:2, filtering is carried out, freezing crystallization is carried out, columnar crystals are separated, and the high-gamma-aminobutyric-acid-content tea extract is obtained. The method has the advantages that the yield of the gamma-aminobutyric-acid tea extract is high, and the content of gamma aminobutyric acid in the gamma-aminobutyric-acid tea extract is high.

Description

technical field [0001] The invention relates to the technical field of plant stem cell culture, in particular to a preparation method of high-content gamma-aminobutyric acid tea extract. technical background [0002] γ-aminobutyric acid (Gamma Aminobutyric Acid, GABA) is a non-protein amino acid, which is mainly used in the pharmaceutical industry for its effects of lowering blood pressure, anticonvulsants, analgesia and improving memory. In the cosmetic industry, γ-aminobutyric acid exhibits excellent moisturizing and anti-wrinkle effects, and is widely used in various moisturizing or anti-wrinkle cosmetics. [0003] After a certain period of anaerobic treatment of fresh tea leaves, γ-aminobutyric acid will be produced. γ-aminobutyric acid in tea is formed by deacidification of L-glutamic acid under the catalysis of L-glutamic acid decarboxylase. At present, both at home and abroad, fresh tea leaves are processed to increase the content of γ-aminobutyric acid. The main tre...

Claims

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Application Information

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IPC IPC(8): A61K8/97A61Q19/00A61Q19/08C12N5/04
Inventor 毕永贤陈玉燕王丽娜镇晓华蒋丽刚
Owner PROYA COSMETICS
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