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Trichoderma rufobrunneum P-T 8155 and uses thereof

A technology of Trichoderma maroon and its application, applied in the field of Trichoderma maroon and its biological control of plant diseases, microorganisms, can solve the problems of chemical pesticide environmental pollution, etc.

Inactive Publication Date: 2015-10-21
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Pathogens are a major "killer" that affects plant growth and development and leads to crop yield reduction. Since the invention of Bordeaux mixture, chemical prevention and control has been the most important measure to control the occurrence, prevalence, damage, and disaster of plant diseases; Pesticide application increases the risk of environmental pollution caused by chemical pesticides. Therefore, the biological control of diseases by using the interaction between biological species has become the development direction of plant disease control

Method used

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  • Trichoderma rufobrunneum P-T 8155 and uses thereof
  • Trichoderma rufobrunneum P-T 8155 and uses thereof
  • Trichoderma rufobrunneum P-T 8155 and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Isolation and purification of embodiment 1 bacterial strain

[0047] Drop 75% ethanol and sterile water into the groove of a sterile cuvette, pick a mature subsea and place it in the ethanol in the cuvette for surface disinfection, rinse with sterile water, and rinse with sterile water. Crush the ascus with tweezers, pick the ascus shell containing the ascus into another sterile cuvette, further crush the ascus, stir well, spread the suspension evenly on the water agar medium, and incubate at room temperature. Place them under a dissecting microscope for observation every other day, pick out germinated single spores with a sterile dissecting needle, transfer them to PDA medium containing ampicillin (2mg / L), and place them in dark culture at 25°C until the growth of Trichoderma After the ascospores germinate and colonies are formed, transfer the actively growing part of the culture to a PDA test tube. When the slope is full of colonies and spores are produced, store them...

Embodiment 2

[0048] The species identification of embodiment 2 bacterial strains

[0049] (1) Morphological identification:

[0050] Observe and record the shape of subunits and the color change of subunits in 3% KOH aqueous solution under a dissecting microscope, and then select mature subunits and place them on a cryostat (Jinhua Yidi YD-1508A) to make a thickness of 5–15 μm The slices were observed and recorded under an optical microscope to record the internal structure of the sub-seat and its color response in 3% KOH aqueous solution. Pick a single ascus shell to make a tablet, observe and record the internal structure of the ascus and ascospores and their color changes in 3% KOH aqueous solution.

[0051] Bacterial blocks with a diameter of 5mm were taken from the actively growing part of the colonies grown at 25°C for 3 days, and transferred to three kinds of media: PDA, CMD, and SNA, and cultured at 15°C, 25°C, and 30°C for 3 days, respectively. Measure the radius of the colony, ...

Embodiment 3

[0072] The preservation of embodiment 3 Trichoderma strains

[0073] The culture was deposited in the General Microbiology Center of China Committee for the Collection of Microorganisms, date of preservation: April 1, 2015, and the preservation number is CGMCC No.10646.

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Abstract

The present invention relates to a strain of Trichoderma rufobrunneum P-T 8155, wherein the strain is preserved in the China General Microbiological Culture Collection Center and has the preservation number of CGMCC No.10646. According to the present invention, the Trichoderma rufobrunneum P-T 8155 has characteristics of strong habitat adaptability, fast growth and high sporulation capacity, can be used for inhibition of Botrytis cinerea, Sclerotinia sclerotiorum, Pythium aphanidermatum, Phytophthora capsici and Rhizoctonia solani, is subjected to hyperparasitism on the hypha of the Botrytis cinerea, Sclerotinia sclerotiorum, Pythium aphanidermatum and Rhizoctonia solani so as to effectively and rapidly degrade the hypha, can further produce non-volatile substance to inhibit the plant pathogens, and further has potential and developable biological prevention and control values.

Description

technical field [0001] The invention belongs to the field of microorganisms, and more specifically relates to a kind of Trichoderma maroon and its application in the field of biological control of plant diseases. Background technique [0002] Pathogens are a major "killer" that affects plant growth and development and leads to crop yield reduction. Since the invention of Bordeaux mixture, chemical prevention and control has been the most important measure to control the occurrence, prevalence, damage, and disaster of plant diseases; Pesticide application increases the risk of environmental pollution caused by chemical pesticides. Therefore, the biological control of diseases by using the interaction between biological species has become the development direction of plant disease control. [0003] Since Weindling first discovered in 1932 that Trichoderma lignorum can parasitize certain plant pathogens and reduce their damage, related research progress has accelerated. Accord...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
CPCA01N63/30C12N1/14C12N1/145C12R2001/885
Inventor 庄文颖秦文韬朱兆香宋霞
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI