Glycyrrhizia polysaccharide liposome preparation method

A technology of glycyrrhizin and liposomes, which is applied in the directions of liposome delivery, medical preparations containing inactive ingredients, and medical preparations containing active ingredients, etc., can solve the problem that the preparation of glycyrrhizin liposomes does not have too much guiding value and other problems, to solve the effect of sticking to the wall and avoiding being difficult to dissolve

Active Publication Date: 2015-11-04
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, because the structure of polysaccharides has its own characteristics, such as the type of monosaccharide, the connection site, the configuration of monosaccharide and glycosidic bonds, and the number of repeating units, etc., will be different due to the different types of polysaccharides extracted.

Method used

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  • Glycyrrhizia polysaccharide liposome preparation method
  • Glycyrrhizia polysaccharide liposome preparation method
  • Glycyrrhizia polysaccharide liposome preparation method

Examples

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Embodiment 1

[0051] Embodiment 1 Ethanol injection method prepares GUPL

[0052] Accurately weigh 0.4g of soybean lecithin, 0.05g of cholesterol and 800.1g of Tween, dissolve it in 10mL of absolute ethanol, and ultrasonically dissolve it fully; -1 The PBS (pH 7.4) solution of licorice polysaccharide was slowly introduced into the former, kept at 45°C and continued to shake for 30 minutes; placed in a 250mL round bottom flask, evaporated under reduced pressure at 50°C to remove ethanol, and then placed in an ultrasonic cell pulverizer for ultrasonic treatment (intensity 40% , open in 2.5s, stop in 2.5s, and act for 15min), so that the glycyrrhizic polysaccharide is fully and evenly integrated into the liposome, and also makes the particle size of the glycyrrhizic polysaccharide liposome more uniform, thereby obtaining the glycyrrhizic polysaccharide liposome suspension Then extrude it respectively through microporous membranes with pore diameters of 0.45 μm and 0.22 μm, respectively repeati...

Embodiment 2

[0054] Embodiment 2 film dispersion method prepares GUPL

[0055] Accurately weigh 0.4g of soybean lecithin, 0.05g of cholesterol and 800.1g of Tween, dissolve it in 10mL of chloroform and methanol (1:1) solution, and ultrasonically dissolve it fully; place it in a 250mL round bottom flask, and evaporate it under reduced pressure at 50°C Organic solvent, when a uniform film is formed on the wall of the flask, introduce 20mL of -1 The PBS (pH 7.0) solution of licorice polysaccharide was evaporated under reduced pressure at 50°C to elute the bottle wall film; hydrated at 25°C for 0.5h; then placed in an ultrasonic cell pulverizer for ultrasonic treatment (intensity 40%, 2.5s on, 2.5 s stop, and act for 15min), the glycyrrhizic polysaccharide is fully and evenly fused in the liposome, and the particle diameter of the glycyrrhizic polysaccharide liposome is also made more uniform simultaneously, thereby obtains the glycyrrhizic polysaccharide liposome suspension; Then it is squeez...

Embodiment 3

[0057] Embodiment 3 Ammonium sulfate gradient method prepares GUPL

[0058] Accurately weigh 0.4g of soybean lecithin, 0.05g of cholesterol and 800.1g of Tween, dissolve it in 10mL of absolute ethanol, and ultrasonically dissolve it fully; under the vibration of a water bath shaker at 50°C, introduce the former into 10mL of 10% ammonium sulfate solution , then placed in a 250mL round-bottomed flask, evaporated under reduced pressure at 50°C to remove the organic solvent; dialyzed in PBS (pH 7.0) for 24h; -1 The PBS (pH 7.0) solution of licorice polysaccharide was slowly introduced into the former, kept at 50°C and continued to shake for 30 minutes; then placed in an ultrasonic cell pulverizer for ultrasonic treatment (intensity 40%, 2.5s on, 2.5s off, 20 minutes of action), so that the licorice polysaccharide Fully and evenly fused into the liposomes, and at the same time make the particle size of the licorice polysaccharide liposomes more uniform, so as to obtain the licorice...

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Abstract

The present invention discloses a glycyrrhizia polysaccharide liposome preparation method, reverse-phase evaporation method is used, the established technological conditions are as follows: the mass ratio of soybean lecithin to cholesterol is 2: 1-10: 1, the mass ratio of soybean lecithin to glycyrrhizia polysaccharide is 5: 1-25: 1, the mass ratio of Tween 80 to soybean lecithin is 1: 1-1: 20, the water bath temperature is 25-65DEG C, and ultrasonic time is 5min-25min. According to the method, for the first time, the glycyrrhizia polysaccharide is prepared as liposome, by response surface ptimization, raw materials can be saved to the greatest degree, meanwhile higher drug encapsulation efficiency (up to 79.12%) can be obtained, and combination with ultrasound effect, the prepared liposome is more stable and more uniform, and the average particle diameter is 136.4 +/-0.151nm.

Description

technical field [0001] The invention belongs to the technical field of medicine preparation, and in particular relates to a preparation method of licorice polysaccharide liposome. Background technique [0002] Licorice has a long history of medicinal use in my country and is a tonic Chinese herbal medicine. Glycyrrhiza polysaccharide (GP) is one of the main active ingredients of licorice, and it is a kind of α-D-pyranose extracted from licorice. The polysaccharide components are composed of rhamnose, dextran, arabinose and semi Lactose is composed of dextran as the main chain. The polysaccharides, whose basic structure is a single glucan linked by α-(1,4) bonds, have the functions of anti-inflammation, enhancing body immunity, etc., and the morphological structure of the glycyrrhiza polysaccharide molecule is a helical structure image. [0003] Liposomes (liposomes) are artificially synthesized bilayer monolayer phospholipids or concentric microcircular lipid capsules sepa...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K31/715A61K47/24A61P29/00A61P37/04
Inventor 武毅李恩涛王绍琛胡元亮刘家国王德云李友英高珍珍熊文牛亚乐
Owner NANJING AGRICULTURAL UNIVERSITY
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