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Sample pretreatment method and application of flavonoid component of dracocephalum heterophyllum benth

A kind of pretreatment technology of fennel flavonoids and samples, which is applied in the field of sample pretreatment of fennel flavonoids components, can solve the problems that have not been reported in the literature, and achieve the effect of low price, stable and controllable quality, and simple process

Inactive Publication Date: 2015-11-11
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no report on the preparation of antiviral active flavonoids from P. heterophylla. Chinese patent (CN103070916B) reports that ethanol extraction and macroporous resin treatment are used to enrich flavonoids from P. heterophylla for the treatment of hypertension. However, So far, there has been no literature report on the pretreatment method of obtaining large-scale anti-hepatitis virus active flavonoids from P. heterophylla

Method used

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  • Sample pretreatment method and application of flavonoid component of dracocephalum heterophyllum benth
  • Sample pretreatment method and application of flavonoid component of dracocephalum heterophyllum benth

Examples

Experimental program
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Embodiment 1

[0032] Example 1 A sample pretreatment method for the flavonoids of isoflavones, comprising the following steps:

[0033] ⑴Liquid-liquid distribution and enrichment:

[0034] 1.0Kg of the plant ethanol extract of Indigo isophyllum was dispersed with 30 times of its mass in analytically pure water to obtain a dispersion, which was extracted 3 times with 30 times of its volume of ethyl acetate, and combined to obtain an ethyl acetate extract; ethyl acetate The extract was dried under reduced pressure to obtain 162.1 g of the medium polar fraction of Chrysanthemum isophyllum.

[0035] Among them: Alcoholic extract of Pseudophyllum plant refers to drying and pulverizing the Pseudophyllum plant, adding 8 times its mass in an ethanol solution with a volume concentration of 95%, soaking for 10 hours, heating and refluxing at 80°C for 2 extractions, each time for 3 hours , to obtain an extract, which is dried under reduced pressure until it is in the form of a paste.

[0036] The ...

Embodiment 2

[0047] Example 2 A sample pretreatment method for the flavonoids of isoflavones, comprising the following steps:

[0048] ⑴Liquid-liquid distribution and enrichment:

[0049] 5.0Kg of the plant ethanol extract of Indigo isophyllum was dispersed with 50 times of its mass in analytically pure water to obtain a dispersion, which was extracted 3 times with 50 times of its volume of ethyl acetate, and combined to obtain an ethyl acetate extract; ethyl acetate The extract was dried under reduced pressure to obtain 822.4 g of the medium polar fraction of Qinglan isophyllum.

[0050] Among them: Alcoholic extract of Pseudophyllum plant refers to drying and pulverizing the Pseudophyllum plant, adding 20 times its mass in a methanol solution with a volume concentration of 95%, soaking it for 15 hours, heating and refluxing at 90°C for 4 times, each time for 3 hours , to obtain an extract, which is dried under reduced pressure until it is in the form of a paste.

[0051] The reduced-...

Embodiment 3

[0062] Example 3 A sample pretreatment method for the flavonoids of isoflavones, comprising the following steps:

[0063] ⑴Liquid-liquid distribution and enrichment:

[0064] 2.0Kg of the plant ethanol extract of Indigo isophyllum is dispersed with 40 times of its mass in analytically pure water to obtain a dispersion, which is extracted 3 times with 40 times of its volume of ethyl acetate, and combined to obtain an ethyl acetate extract; ethyl acetate The extract was dried under reduced pressure to obtain 318.6 g of the medium polar fraction of Qinglan isophyllum.

[0065] Among them: Alcohol extract of Cymbidium heterophyllum plant refers to drying and crushing Chrysanthemum heterophyllum plant in the shade, adding 14 times its mass in 95% ethanol solution and soaking for 12 hours, heating and refluxing at 85°C for 3 times, each time for 3 hours , to obtain an extract, which is dried under reduced pressure until it is in the form of a paste.

[0066] The reduced-pressure...

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Abstract

The invention relates to a sample pretreatment method of flavonoid component of dracocephalum heterophyllum benth. The sample pretreatment method comprises the following steps: (1) carrying out liquid-liquid partitioning enrichment, namely, carrying out dispersion, extraction and vacuum drying on pohytol extract of dracocephalum heterophyllum benth to obtain medium polar component part of dracocephalum heterophyllum benth; (2) removing purities by adopting micro-reticular resin, namely, dissolving the medium polar component part of dracocephalum heterophyllum benth, filtering, separating by a micro-reticular resin column, eluting, collecting the eluate, and carrying out vacuum drying on the eluate to obtain the yellow flavonoid component of dracocephalum heterophyllum benth; (3) and carrying out chromatography isolation by adopting a silicagel column, namely, separating the flavonoid component crude product of dracocephalum heterophyllum benth by adopting the silicagel column chromatography, eluting, detecting by adopting thin-layer chromatography, mixing, and collecting fraction with the Rf value being 0.1-0.8, and carrying out vacuum drying on the fraction, thus obtaining the flavonoid component of dracocephalum heterophyllum benth, which is in the form of yellow powder. The technology is simple, and easy in realizing scale production. The invention further discloses an application of the flavonoid component of dracocephalum heterophyllum benth in preparing a drug or health food for resisting hepatitis virus.

Description

technical field [0001] The invention relates to a preparation method of flavonoid components, in particular to a sample pretreatment method and application of the flavonoid components of isoflavone. Background technique [0002] different leaf green orchid ( Dracocephalum heterophyllum Benth) belongs to Labiatae ( Labiatae ) Cymbidium ( Dracocephalum ) plants, mainly distributed in temperate Asia. The plant is widely used in the folk, and has the effects of calming the liver, clearing away heat, and treating high blood pressure, lymphadenitis, and cough due to lung heat. Modern studies have shown that the chemical constituents in Indigo isophylla mainly include phenylpropanoids, flavonoids and terpenoids, among which flavonoids are well known for their significant antioxidant, cardiovascular and cerebrovascular disease, anti-tumor and anti-viral activities. [0003] At present, there is no report on the preparation of antiviral active flavonoids from P. heterophylla. C...

Claims

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Application Information

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IPC IPC(8): A61K36/53A61P31/12A61P1/16A23L1/29A23L33/00
Inventor 王启兰张莉党军陶燕铎王蒲于瑞涛梅丽娟郑威卢晓华
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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