Sharpleaf galangal fruit extract capable of treating chronic kidney diseases and applications thereof

A technology for chronic kidney disease and Yizhiren, which is applied to medical preparations containing active ingredients, drug combinations, plant raw materials, etc., can solve the problems of poor patient compliance, poor patient efficacy, and long course of treatment for western medicine, and achieve convenient administration. , good renal protection, low toxicity effect

Active Publication Date: 2015-11-11
HAINAN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the methods used by Western medicine to treat chronic nephritis proteinuria include hormones, immunosuppressants, ACEI / ARB drugs, etc. Although some clinical curative effects have been achieved, there are still quite a few patients with poor curative effects, and the treatment course of Western medicine is long , easy to rebound after drug withdrawal, more side effects, poor patient compliance

Method used

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  • Sharpleaf galangal fruit extract capable of treating chronic kidney diseases and applications thereof
  • Sharpleaf galangal fruit extract capable of treating chronic kidney diseases and applications thereof
  • Sharpleaf galangal fruit extract capable of treating chronic kidney diseases and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1, a kind of Yizhiren extract with the effect of treating chronic kidney disease, it is prepared by the following method:

[0029] Take a certain quality of nootropic fruit, break it, separate and remove the nootropic husk to get nootropic kernels, add 95% volume concentration ethanol of 10 times the volume of nootropic kernels, reflux extraction for 3 times, each time for 2 hours, collect and merge The extract is applied to a macroporous resin, first eluted with 60% ethanol to remove impurities, then eluted with 90% high-concentration ethanol, and concentrated into an extract, which is separated by a silica gel column, and the extract is separated by a volume ratio It was eluted with 19:1 petroleum ether-ethyl acetate, the eluate was concentrated, and crystallized at 4°C to obtain the extract of P. japonicus. The extract was identified as citrus ketone by modern spectroscopy, and the specific data are as follows.

[0030] Colorless crystals (4°C), ESI-MSm / z218[M+H]+; ...

Embodiment 2

[0033] 1, a kind of Yizhiren extract with the effect of treating chronic kidney disease, it is prepared by the following method:

[0034] Take a certain quality of nootropic fruit, break it, separate and remove the nootropic husk to get nootropic kernels, add 70% volume concentration ethanol of 12 times the volume of nootropic kernels, reflux extraction twice, each time for 2 hours, collect and merge The extract is applied to a macroporous resin, first eluted with a volume concentration of 50% ethanol to remove impurities, then eluted with a volume concentration of 80% high-concentration ethanol, and concentrated into an extract, which is separated by a silica gel column, and the volume ratio It is 100:1~100:10 petroleum ether-ethyl acetate gradient elution in sequence, the eluate is concentrated, and crystallized at 4°C to obtain the extract of P. japonicus. The extract is identified as narone by modern spectroscopy, which is the same as in Example 1 by modern spectroscopy. ...

Embodiment 3

[0035] Embodiment 3 treats the pharmacodynamics test of chronic kidney disease

[0036] 1. Drug effect research on adenine-induced kidney injury in rats: 48 mice were randomly divided into 6 groups, i.e. normal group, model group, positive drug group, and narone prepared in Example 1 of the present invention Compound high, medium and low dosage groups, 8 rats in each group, were administered according to Table 1.

[0037] Table 1 Dosing regimen of Yizhiren extract for drug efficacy study on adenine-induced kidney injury in rats

[0038]

[0039]14 days after administration. Rats in each group were placed in metabolic cages, fasted without water, collected 24-hour urine, and measured 24-hour urine volume and urine protein (UP 24h ). After fasting for 6 hours, the rats were anesthetized with ether, blood was collected from the abdominal aorta, and the serum was separated to measure blood urea nitrogen (BUN), serum creatinine (Scr), serum superoxide dismutase (SOD) and malo...

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Abstract

The invention discloses a sharpleaf galangal fruit extract capable of treating chronic kidney diseases. The extract is prepared by the following steps: grinding sharpleaf galangal fruits to remove the shells, adding a certain amount of ethanol, carrying out reflux extraction, collecting the extract, making the extract go through macroporous resin, eluting the macroporous resin by low-concentration ethanol to remove the impurities, then eluting the macroporous resin by high-concentration ethanol, condensing to obtain extract, separating the extract in a silica gel column, and carrying out crystallization so as to obtain the sharpleaf galangal fruit extract. According to the pathogenesis of chronic kidney diseases and on the basis of traditional Chinese medicine theory, the inventor researches the chemical components and the clinical curative effect of sharpleaf galangal fruit. The results of a great number of experiments show that the prepared sharpleaf galangal fruit extract (nootkatone) can prominently reduce proteinuria, can also obviously reduce the levels of serum creatinine and urea nitrogen, has a good kidney protective effect, and can be used to prevent and treat chronic kidney diseases.

Description

technical field [0001] The invention relates to a traditional Chinese medicine, in particular to an extract of yizhiren which has the function of treating chronic kidney disease and its application. Background technique [0002] Modern medicine believes that the kidney is the organ that produces urine, and increased nocturia is a manifestation of increased kidney filtration and impaired concentration. The main cause of nocturia is chronic kidney disease (Chronic KidneyDisease, CKD), such as chronic glomerulonephritis, chronic pyelonephritis, hypertension, renal arteriosclerosis and chronic renal insufficiency. Due to decreased renal function, the kidneys cannot completely excrete metabolites in the body during the day, and need to continue to excrete them at night, resulting in increased nocturia. [0003] Chronic kidney disease is a serious threat to human life and health, and its incidence is increasing year by year. It has become a global public health event and has attr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9062A61P13/12
Inventor 李永辉谭银丰张俊清
Owner HAINAN MEDICAL COLLEGE
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