Deep-sea Bacillus circulans and application thereof in suppression of aflatoxin

A technology of bacillus circulans and deep-sea strains, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve problems such as unsatisfactory effects, high cost, and nutrient loss, and achieve good biological safety and stable properties

Inactive Publication Date: 2015-11-11
HARBIN INST OF TECH AT WEIHAI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The preferred method for effective prevention and control of aflatoxin-contaminated feed and grain is toxin suppression, that is, to inhibit the production of aflatoxin in feed and grain, rather than detoxification, that is, to remove the aflatoxin that has been produced, because detoxification is not only costly , the effect is not satisfactory, it is difficult to eradicate, and it will lead to the loss of nutrition

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: the liquid fermentation of deep-sea Bacillus circulans FA13 bacterial strain

[0014] Inoculate the liquid seeds of the FA13 strain cultivated overnight into a liquid medium containing 0.5-1.5% yeast extract, 0.1-0.3% maltose and 1.0-3.0% glucose at 20-36 degrees, 100- Cultivate at 120 rpm for 6-12 days, finish the fermentation, and centrifuge the fermentation broth. The obtained cell-free supernatant is the fermentation broth containing active substances that can effectively inhibit aflatoxin, which can be directly used to prevent and control Aspergillus flavus Toxin pollution, or processed into dry powder for the prevention and control of aflatoxin pollution.

Embodiment 2

[0015] Embodiment 2: the fermented liquid of deep-sea Bacillus circulans FA13 bacterial strain and its dilution suppress the activity of aflatoxin

[0016] The cell-free fermentation supernatant obtained in Example 1 is added with the same amount of nutrients as the fermentation medium as the culture medium for cultivating aflatoxin-producing bacteria, and the cell-free fermentation supernatant obtained in Example 1 is used for fermentation After the culture medium was diluted 30 times and 40 times respectively, it was used as the culture solution for cultivating aflatoxin-producing bacteria, and the comparison was a fermentation medium. After inoculating 1% of the spore suspension of aflatoxin-producing bacteria in the above-mentioned medium, the culture medium was inoculated at 28 Cultivate for 6 days, collect the mycelium by centrifugation, after weighing, extract the aflatoxin intermediate product in the mycelium with methanol-sodium hydroxide solution, measure the absorban...

Embodiment 3

[0017] Embodiment 3: the temperature stability of the active substance in the fermented liquid of Bacillus circulans FA13 bacterial strain

[0018] The cell-free fermentation supernatant that embodiment 1 obtains is at 60,80 o After heat treatment at C temperature for 2 hours, cool to room temperature naturally, and at 121 o After autoclaving at C for 30 minutes, cool down to room temperature naturally. After inoculating the 1% spore suspension of aflatoxin-producing bacteria with the above-mentioned fermented liquid, the fermented liquid without temperature treatment, and the blank medium, respectively, in Cultivate at 28 degrees for 6 days, observe the spore germination, the results show that even at 121 o The fermentation broth sterilized under high pressure for 30 minutes under C can also completely inhibit the spore germination of aflatoxin-producing bacteria and the production of aflatoxin like the fermentation broth without temperature treatment, indicating that the an...

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Abstract

The invention provides a deep-sea bacterial strain capable of preventing and controlling aflatoxin, and particularly relates to a Bacillus circulans FA13 bacterial strain which is formed by separating light-yellow ooze sediment in water of 3203m in depth in South Atlantic, collected in the China General Microbiological Culture Collection Center and numbered as CGMCC No.10663. After the FA13 bacterial strain is fermented under optimized conditions and obtained fermentation supernate is diluted by 30 times, the fermentation supernate can still kill spores of aflatoxin producing bacteria and suppress producing of aflatoxin 100%, in other words, the fermentation supernate has capability of suppressing production of aflatoxin from a growing source, has no suppression effect on intestinal flora and unicellular fungus saccharomyces cerevisia and has higher using safety; active metabolite in fermentation liquid is stable in property and resistant to high temperature, acid and alkali and degrading of pepsin, trypsin and chymotrypsin. The FA13 bacterial strain and a fermentation product thereof can be used for effectively preventing and controlling aflatoxin pollution of feed, grain and field crops.

Description

technical field [0001] The invention relates to the technical field of deep-sea microbial technology and mycotoxin biological control, in particular to a strain of deep-sea bacillus circulans and its application for inhibiting aflatoxin. Background technique [0002] Aflatoxin is currently the most toxic mycotoxin found in nature, and has strong carcinogenic, teratogenic and immunosuppressive properties, and is listed as a class I carcinogen by the International Agency for Cancer. It is mainly produced by filamentous fungi such as Aspergillus flavus and Aspergillus parasiticus, which can widely pollute food and feed, etc. After humans and animals eat food and feed contaminated by aflatoxin, it can seriously endanger the health of humans and animals. Therefore, how to effectively prevent Controlling aflatoxin pollution is a major issue that needs to be solved urgently in the world. [0003] The preferred method for effective prevention and control of aflatoxin-contaminated f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/09
Inventor 闫培生王文威邵宗泽王凯曹立新贾文文
Owner HARBIN INST OF TECH AT WEIHAI
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