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Aspergillus oryzae fermentation liquid, liquid glucose prepared therewith through degradation of straw powder, and preparation method and application of the liquid glucose

A technology of Aspergillus oryzae and fermentation liquid, which is applied in the fields of fermentation engineering and enzyme engineering, and can solve unseen problems

Active Publication Date: 2015-11-11
JIANGSU YIMING BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Aspergillus oryzae is a feed-grade microbial additive strain that can be directly fed to animals recognized by the US Food and Drug Administration (FDA), the American Feed Standards Association (AAFCO) and the Ministry of Agriculture of my country, but no lignin peroxide secretion by Aspergillus oryzae has been seen Enzyme patents and related literature reports

Method used

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  • Aspergillus oryzae fermentation liquid, liquid glucose prepared therewith through degradation of straw powder, and preparation method and application of the liquid glucose
  • Aspergillus oryzae fermentation liquid, liquid glucose prepared therewith through degradation of straw powder, and preparation method and application of the liquid glucose
  • Aspergillus oryzae fermentation liquid, liquid glucose prepared therewith through degradation of straw powder, and preparation method and application of the liquid glucose

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Embodiment 1 Aspergillus oryzae fermented liquid

[0076] Aspergillus oryzae fermented liquid contains lignin peroxidase enzyme activity 1000U / L and exocellulase enzyme activity 100U / L; Said aspergillus oryzae fermented liquid is raw material with rice straw powder, aspergillus oryzae is bacterial strain, expands successively through test tube Culture, liquid shake flask culture, seed tank seed culture and liquid fermentation culture process.

Embodiment 2

[0077] Embodiment 2 Aspergillus oryzae fermented liquid

[0078] Aspergillus oryzae fermentation broth contains lignin peroxidase enzyme activity 100U / L and exo-cellulase enzyme activity 300U / L; said Aspergillus oryzae fermentation broth is made of rice straw powder, and Aspergillus oryzae is a bacterial strain prepared according to the following steps :

[0079] a. test tube expansion culture: inoculate Aspergillus oryzae strains in potato glucose medium, and cultivate them at 35°C for 5 days to obtain Aspergillus oryzae test tube slant spores;

[0080] b. Liquid shake flask culture: Inoculate the slanted spores of Aspergillus oryzae test tubes into the shake flasks equipped with liquid shake flask culture medium, and cultivate them for 30 hours on a shaking table with a rotation speed of 110 rpm and a temperature of 30° C. to obtain liquid shake flask bacteria kind;

[0081] c. Seed culture in first-level seed tanks: inoculate the strains in liquid shake flasks into the me...

Embodiment 3

[0083] Embodiment 3 Aspergillus oryzae fermented liquid

[0084] The Aspergillus oryzae fermentation broth contains lignin peroxidase enzyme activity 500U / L and exocellulase enzyme activity 130U / L; the Aspergillus oryzae fermentation broth is prepared according to the following method:

[0085] a. test tube expansion culture: inoculate Aspergillus oryzae strains in potato glucose medium, cultivate 3d at a temperature of 25°C, and then preserve at 10°C to obtain Aspergillus oryzae test tube slant spores;

[0086] b. Liquid shake flask culture: Inoculate a ring of Aspergillus oryzae test tube slant spores into a 250mL Erlenmeyer flask containing 100mL of liquid shaker flask culture medium, and culture it on a shaker at a temperature of 38°C for 40 hours at a rotating speed of 50 rpm. , to prepare liquid shake flask strains;

[0087] C. First-class seed tank seed culture: by inoculum size is 12%, inoculate liquid shaking flask bacterial classification in the seed tank culture me...

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Abstract

The invention relates to an aspergillus oryzae fermentation liquid, which contains lignin peroxidase with enzymatic activity being 100-1000 U / L and cellobiohydrolase with enzymatic activity being 100-300 U / L. The aspergillus oryzae fermentation liquid is prepared with straw powder as a raw material and aspergillus oryzae as the strain and through the processes of enlarge cultivation in test tubes, liquid shake-flask culture, seed culture in seed pots and liquid fermentation cultivation. The invention also provides the technology of preparing liquid glucose with the straw powder as a main raw material through the aspergillus oryzae fermentation liquid with addition of a hydrogen peroxide solution through degradation of straw powder. The technology is short in production period, is less in occupied area, is low in production cost and is high in yield of glucose. The prepared liquid glucose can be used as a raw material for fermenting biogas.

Description

technical field [0001] The invention belongs to the fields of fermentation engineering and enzyme engineering, and in particular relates to an aspergillus oryzae fermentation liquid, a sugar liquid prepared by degrading rice straw powder from the fermentation liquid, a preparation method and an application. Background technique [0002] my country is a large agricultural country, with an annual output of more than 600 million tons of crop straw. Except for a small amount used in the textile and papermaking industries, most of them are dumped into the environment in the form of accumulation and incineration, which wastes resources and causes great harm to the environment. . Straw cellulose is an important source of renewable energy, and straw can be developed and utilized to solve the problem of rural energy consumption; among them, using straw for biogas fermentation is the most effective means for human beings to use biomass energy rationally. The application of straw bioma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12N9/08C12P19/14C12P5/02C12R1/69
CPCY02E50/30
Inventor 张志才
Owner JIANGSU YIMING BIOLOGICAL SCI & TECH
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