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A kind of veterinary vaccine and preparation method thereof

A vaccine and immune adjuvant technology, applied in the field of veterinary vaccines and its preparation, can solve the problems of inability to quickly induce high-titer antibody production, antibody titer lower than normal, and antibody level decline, and achieve great potential for development and utilization , solve the side effects of vaccines, and the effect of easy injection

Active Publication Date: 2018-07-17
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, RHD inactivated vaccine is used clinically for prevention, but there are many defects: high titer antibodies cannot be induced quickly after injection, and some rabbits have been infected with rabbit plague virus before producing protective antibodies; the protection period of antibodies is short , the antibody level drops rapidly. If the second immunization is not timely, the antibody titer will soon be lower than the normal value; the storage conditions of the vaccine are relatively strict, and improper storage will easily lead to a decrease in the vaccine titer

Method used

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  • A kind of veterinary vaccine and preparation method thereof
  • A kind of veterinary vaccine and preparation method thereof
  • A kind of veterinary vaccine and preparation method thereof

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preparation example Construction

[0024] Aiming at the problem of developing novel vaccines, the invention provides a veterinary vaccine and a preparation method thereof.

[0025] Pine pollen polysaccharides are composed of monosaccharides such as mannose, glucuronic acid, rhamnose, glucose, galactose, arabinose and fucose. Modern research has proved that pine pollen polysaccharide is one of the nutrients that maintain the special effects of pine pollen, and has very high biological activity. The extraction of polysaccharides from pine pollen is significantly increased after the wall is broken. In the present invention, fresh pine pollen in Mount Tai area is collected in spring, sieved and broken, and the crude polysaccharide is extracted by water extraction and alcohol precipitation. During the extraction process, protein is removed by pepsin and Sevage reagent, which ensures a higher purity of the crude polysaccharide.

[0026] A variety of plant polysaccharides can not only activate T cells, B cells, macrop...

Embodiment 1

[0029] Example 1 Vaccine 1

[0030] A veterinary vaccine, the vaccine is composed of immune adjuvant and antigen aqueous solution with a mass ratio of 4:96.

[0031] The antigen solution was mixed with rabbit haemorrhagic disease virus material with a hemagglutination titer of 12log2 and normal saline at a mass ratio of 1:10, and the antigen solution was obtained after fully homogenizing.

[0032] The immune adjuvant consists of the following raw materials in parts by mass: 5 parts of Taishan pine pollen polysaccharide, 10 parts of propolis, 5 parts of aloe polysaccharide, 8 parts of raspberry polysaccharide, 6 parts of Laojunxu polysaccharide, 9 parts of Dawanhua polysaccharide, hydrophilic 10 parts of surfactant, 0.1 part of polymer micellar substance and 40 parts of water.

[0033] Wherein, the hydrophilic surfactant is a mixture of Span 80 and Pluronic 121; the mass-number ratio of Span 80 and Pluronic 121 is 1 part for Span 80 and 3 parts for Pluronic 121.

[0034] Wher...

Embodiment 2

[0043] Embodiment 2 Adjuvant 2

[0044] A veterinary vaccine, the vaccine is composed of immune adjuvant and antigen aqueous solution with a mass ratio of 11:89.

[0045] The antigen solution was mixed with rabbit haemorrhagic disease virus material with a hemagglutination titer of 11log2 and normal saline at a mass ratio of 1:10, and the antigen solution was obtained after fully homogenizing.

[0046] The immune adjuvant consists of the following raw materials in parts by mass: 30 parts of Taishan pine pollen polysaccharide, 5 parts of propolis, 10 parts of aloe polysaccharide, 14 parts of raspberry polysaccharide, 3 parts of Laojunxu polysaccharide, 4 parts of Dawanhua polysaccharide, hydrophilic 0.1 parts of active surfactant, 10 parts of polymer micelles and 90 parts of water.

[0047] Wherein, the hydrophilic surfactant is a mixture of Span 80 and Pluronic 121; the mass-number ratio of Span 80 and Pluronic 121 is 2.5 parts for Span 80 and 1 part for Pluronic 121.

[004...

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Abstract

The invention discloses a live vaccine and a preparation method thereof. The live vaccine is composed of immune adjuvant and an antigen aqueous solution according to the mass ratio of 4-11 to 89-96. The live vaccine and the preparation method have the advantages of being simple in preparation process, low in cost, simple in component, stable in dosage form, easy to store and inject, convenient to use, good in use effect and free of toxic and side effects, having great development and utilization potential in the animal husbandry, and having wide application prospects.

Description

technical field [0001] The invention relates to a vaccine adjuvant, in particular to a veterinary vaccine and a preparation method thereof. Background technique [0002] Rabbit plague, also known as rabbit viral hemorrhagic disease (RHD), is an acute and highly contagious infectious disease of rabbits caused by rabbit viral hemorrhagic disease virus (RHDV), and all kinds of rabbits are susceptible to it throughout the year. The disease is characterized by respiratory hemorrhage, liver necrosis, parenchymal organ edema, congestion and hemorrhagic changes. Since it was first discovered in Wuxi, Jiangsu Province in 1984, it has spread rapidly to many provinces and cities across the country. So far North Korea, South Korea, Italy, France, Germany, the Czech Republic, the United States and New Zealand and other countries have the prevalence of this disease. The disease has a short incubation period, acute onset, short course of disease, rapid spread, high morbidity and mortalit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61K39/12A61K9/08A61P31/14
Inventor 朱瑞良胡莉萍魏凯彭军钟世勋
Owner SHANDONG AGRICULTURAL UNIVERSITY