All-solid-state acetylcholine sensor and preparation method thereof
An acetylcholine and all-solid-state technology, applied in the field of biosensors, can solve the problems of poor real-time performance of acetylcholine, cumbersome experimental pretreatment, and difficult storage, and achieve the effects of rapid response, stable work, and easy operation
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Embodiment 1
[0066] (1) Immerse one end of two gold wires with a diameter of 0.1mm into a suspension of alumina powder with a diameter of 0.5 microns, the concentration of the alumina suspension is 80g / L, use ultrasonic vibration to polish for 10 minutes, and rinse with deionized water; Then immerse the same end of the gold wire into a suspension of alumina powder with a diameter of 50 nanometers, the concentration of the alumina suspension is 80g / L, use ultrasonic vibration to polish for 10 minutes, and rinse with deionized water; Soak in dilute sulfuric acid solution for 3 minutes, rinse with deionized water;
[0067] (2) adopting 0.3% sodium carboxymethylcellulose solution as surfactant active aqueous solution to put into drying oven after gold wire is cleaned;
[0068] (3) Disperse poly 3,4-hexylenedioxythiophene / polystyrene sulfonic acid (PEDOT / PSS, Clevios P from H.C. Starck, Germany, CAS No.155090-83-8) in deionized water to form a suspension Liquid, the ratio of PEDOT / PSS:H 2 O...
Embodiment 2
[0078] (1) Immerse one end of two platinum wires with a diameter of 0.1mm into a suspension of alumina powder with a diameter of 0.5 microns, the concentration of the alumina suspension is 80g / L, use ultrasonic vibration to polish for 10 minutes, and rinse with deionized water; Then immerse the same end of the gold wire into a suspension of alumina powder with a diameter of 50 nanometers, the concentration of the alumina suspension is 80g / L, use ultrasonic vibration to polish for 10 minutes, and rinse with deionized water; Soak in dilute sulfuric acid solution for 3 minutes, rinse with deionized water;
[0079] (2) Adopting 0.3% sodium carboxymethylcellulose solution as a surfactant active aqueous solution to clean the platinum wire and put it into a dry box for drying;
[0080] (3) Mix aniline and 2,5 dimethoxybenzene to form a copolymer, and the mass ratio of the two is aniline:2,5 dimethoxybenzene=5:1. Then prepare a DMF saturated solution of soluble polyaniline. Dip the ...
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