All-solid-state acetylcholine sensor and preparation method thereof

An acetylcholine and all-solid-state technology, applied in the field of biosensors, can solve the problems of poor real-time performance of acetylcholine, cumbersome experimental pretreatment, and difficult storage, and achieve the effects of rapid response, stable work, and easy operation

Active Publication Date: 2018-01-05
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides an all-solid-state acetylcholine sensor and a preparation method thereof. The all-solid-state acetylcholine sensor solves the problems of poor real-time detection of traditional acetylcholine in vivo, cumbersome experimental pretreatment, and difficult storage, and has the advantages of miniaturization, easy storage, rapid detection, Advantages of easy operation and long use time

Method used

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  • All-solid-state acetylcholine sensor and preparation method thereof
  • All-solid-state acetylcholine sensor and preparation method thereof
  • All-solid-state acetylcholine sensor and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0066] (1) Immerse one end of two gold wires with a diameter of 0.1mm into a suspension of alumina powder with a diameter of 0.5 microns, the concentration of the alumina suspension is 80g / L, use ultrasonic vibration to polish for 10 minutes, and rinse with deionized water; Then immerse the same end of the gold wire into a suspension of alumina powder with a diameter of 50 nanometers, the concentration of the alumina suspension is 80g / L, use ultrasonic vibration to polish for 10 minutes, and rinse with deionized water; Soak in dilute sulfuric acid solution for 3 minutes, rinse with deionized water;

[0067] (2) adopting 0.3% sodium carboxymethylcellulose solution as surfactant active aqueous solution to put into drying oven after gold wire is cleaned;

[0068] (3) Disperse poly 3,4-hexylenedioxythiophene / polystyrene sulfonic acid (PEDOT / PSS, Clevios P ​​from H.C. Starck, Germany, CAS No.155090-83-8) in deionized water to form a suspension Liquid, the ratio of PEDOT / PSS:H 2 O...

Embodiment 2

[0078] (1) Immerse one end of two platinum wires with a diameter of 0.1mm into a suspension of alumina powder with a diameter of 0.5 microns, the concentration of the alumina suspension is 80g / L, use ultrasonic vibration to polish for 10 minutes, and rinse with deionized water; Then immerse the same end of the gold wire into a suspension of alumina powder with a diameter of 50 nanometers, the concentration of the alumina suspension is 80g / L, use ultrasonic vibration to polish for 10 minutes, and rinse with deionized water; Soak in dilute sulfuric acid solution for 3 minutes, rinse with deionized water;

[0079] (2) Adopting 0.3% sodium carboxymethylcellulose solution as a surfactant active aqueous solution to clean the platinum wire and put it into a dry box for drying;

[0080] (3) Mix aniline and 2,5 dimethoxybenzene to form a copolymer, and the mass ratio of the two is aniline:2,5 dimethoxybenzene=5:1. Then prepare a DMF saturated solution of soluble polyaniline. Dip the ...

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Abstract

The invention discloses an all-solid-state acetylcholine sensor, which includes an external reference electrode and a selective electrode. The selective electrode includes a metal wire, a solid electrolyte layer and an acetylcholine sensitive film. One end of the metal wire is sequentially coated There is a detection end of a solid electrolyte layer and an acetylcholine sensitive film, and the acetylcholine sensitive film is coated on a part of the surface of the solid electrolyte layer; the other end of the metal wire is a bare electrical signal output end; the acetylcholine The sensitive membrane contains 2-nitrophenyl octyl ether. The all-solid-state acetylcholine sensor integrates an acetylcholine selective electrode and an external reference electrode, which is convenient for storage and use, and has the advantages of simple operation, rapid response, stable operation and long service life.

Description

technical field [0001] The invention belongs to the field of biosensors, and in particular relates to an all-solid-state acetylcholine sensor and a preparation method thereof. Background technique [0002] According to the current research, acetylcholine is distributed in the central nervous system and peripheral nerves. It can specifically act on various choline receptors, and it will be rapidly decomposed by cholinesterase in the tissue. It has a wide range of effects, but it is selective. not tall. Acetylcholine is mainly synthesized in cholinergic nerve endings, with little intracellular synthesis. [0003] In the central nervous system, acetylcholine mainly exists in the striatum, motor neurons in the anterior horn of the spinal cord, and the ascending activating system of the brainstem reticular structure. Among these parts, acetylcholine has a certain relationship with human learning, memory and recognition. In addition, the content of acetylcholine in the thalamus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30
Inventor 王酉何成李光
Owner ZHEJIANG UNIV
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