Refining process of pond turtle bioactive peptides and application of pond turtle bioactive peptides in preventing liver cirrhosis and resisting fatigue

A technology of active peptide and refining method, which is applied in the prevention of liver cirrhosis and anti-fatigue. In the field of stone turtle active peptide refining method, it can solve the problems of peptide inactivation, poor taste, and long time, and achieve the improvement of enzyme activity. , easy to operate, conducive to the effect of penetration

Active Publication Date: 2015-12-09
广西自贸区睿创贸易有限公司
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Problems solved by technology

[0004] Qinzhou is located in the subtropical coastal area and has a suitable climate. Due to its unique geographical and natural conditions, Qinzhou stone money turtle is closer to natural ecological products with excellent quality. As a rising star, there are currently more than 10,000 stone turtle farmers, with an annual output value of more than 350 million. However, although the supply of goods is sufficient, most of them still stay in a single breeding and sales model. Food, and often rough craft, poor taste, lack of market competitiveness, deep processing is still in a blank state
How to extract, separate and purify the active peptide from the stone turtle is still a major difficulty in the research. The traditional processing method takes a long time to decoct the active ingredients and consumes a lot of energy. The general acid extraction method destroys some amino acids and reduces the product yield. Low, amino acids containing hydroxyl and sulfhydryl groups extracted by alkaline water extraction are prone to racemization, peptides are inactivated, and the separation and purification of peptides is a systematic project. Generally, sedimentation filtration, centrifugal separation, and solvent extraction are very difficult. It is difficult to achieve a good separation effect, so the present invention develops a deep processing method for stone turtles on the basis of a large number of experiments in the early stage. The extraction rate is high, the separation and purification effect is good, and the activity is high. In-depth systematic research on the value of health care has formed a complete system integrating scientific research, production, deep processing and sales. The developed stone turtle active peptide has better health effects of preventing liver cirrhosis, anti-fatigue, and enhancing immunity

Method used

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  • Refining process of pond turtle bioactive peptides and application of pond turtle bioactive peptides in preventing liver cirrhosis and resisting fatigue
  • Refining process of pond turtle bioactive peptides and application of pond turtle bioactive peptides in preventing liver cirrhosis and resisting fatigue
  • Refining process of pond turtle bioactive peptides and application of pond turtle bioactive peptides in preventing liver cirrhosis and resisting fatigue

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Embodiment 1

[0027] The stone turtle active peptide refining method comprises the following steps:

[0028] 1) Raw material pretreatment: kill the stone tortoise, remove the head, remove the viscera, wash 3 times with water, first crush, add acetic acid (1:20W / V) with a pH of about 2.5, soak at 5°C for 24 hours, take out after the expansion is uniform , add water to wash twice until there is no sour smell, drain, add water 8 times to beat until homogenized, add water to make up for weight loss, and set aside;

[0029] 2) Enzymolysis: add 1% citric acid to the homogenate as the extraction medium of the enzymatic hydrolysis method to adjust the pH to 3, add 1% pepsin, enzymolyze at 4°C for 5 hours, stir for 15 minutes with a magnetic stirrer every 1 hour, heat to inactivate the enzyme Live, get the enzymatic hydrolyzate of stone turtle;

[0030] 3) Multi-stage membrane separation and purification:

[0031] ①Microfiltration: Take the enzymatic hydrolysis solution of the stone turtle, add wa...

Embodiment 2

[0037] The stone turtle active peptide refining method comprises the following steps:

[0038]1) Raw material pretreatment: kill the stone tortoise, remove the head, remove the internal organs, wash 4 times with water, first crush, add acetic acid (1:20W / V) with a pH of about 2.5, soak at 10°C for 24 hours, take out after the expansion is uniform , add water to wash repeatedly 3 times until there is no sour taste, drain, add water 9 times to beat until homogenized, add water to make up for weight loss, and set aside;

[0039] 2) Enzymolysis: add 1% citric acid to the homogenate as the extraction medium of the enzymatic hydrolysis method to adjust the pH to 3, add 2% pepsin, enzymolysis at 4°C for 7 hours, stir for 15 minutes with a magnetic stirrer every 1 hour, heat to inactivate the enzyme Live, get the enzymatic hydrolyzate of stone turtle;

[0040] 3) Multi-stage membrane separation and purification:

[0041] ①Microfiltration: Take the enzymatic hydrolysis solution of th...

Embodiment 3

[0047] The stone turtle active peptide refining method comprises the following steps:

[0048] 1) Raw material pretreatment: kill the stone tortoise, remove the head, remove the viscera, wash 3 times with water, first crush, add acetic acid (1:20W / V) with a pH of about 2.5, soak at 10°C for 24 hours, take out after the expansion is uniform , add water to wash repeatedly 3 times until there is no sour taste, drain, add water 10 times to beat until homogenized, add water to make up for weight loss, and set aside;

[0049] 2) Enzymolysis: add 1% citric acid to the homogenate as the extraction medium of the enzymatic hydrolysis method to adjust the pH to 3, add 2% pepsin, enzymolyze at 4°C for 6 hours, stir with a magnetic stirrer for 15 minutes every 1 hour, heat to inactivate the enzyme Live, get the enzymatic hydrolyzate of stone turtle;

[0050] 3) Multi-stage membrane separation and purification:

[0051] ①Microfiltration: Take the enzymatic hydrolysis solution of the stone...

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Abstract

The invention discloses a refining process of pond turtle bioactive peptides. Acetic acid immersion pretreatment is firstly adopted, expanding tissue is softened, part of impurity protein is removed, an enzymolysis method is adopted for treatment, citric acid is added to be used as an enzymolysis medium, the extraction rate of the bioactive peptides is higher than 25% while the extraction rate in the prior art is only about 10%, and defects of time consumption and energy dissipation with a conventional processing method, damage to amino acid with a general acid hydrolyzation method, high probability of racemization with an alkali hydrolysis method and the like are overcome. Besides, a multilevel membrane separation technology is combined, the selectivity is high, few by-products are produced, the adaptability is high, the purity of the separated bioactive peptides is higher than 75%, the molecular weight is smaller than 10 KD, the bioactive peptides can enter an organism circulation system completely and are easy to absorb, and however, molecular weight is not uniformly distributed and the absorption effect is poor with a commonly used centrifugal and precipitation filtering method. Desalination and debitterization are also completed in the enzymolysis, microfiltration and multilevel ultrafiltration processes, and the prepared pond turtle bioactive peptides have shallower color, don't have foreign smell, have uniform molecular weight, are safe and non-toxic, and have wide application prospect in health care of liver cirrhosis prevention, fatigue resistance, immunity enhancing and the like.

Description

technical field [0001] The invention relates to the technical field of biological health food, in particular to the refining method of Shigui active peptide and its application in preventing liver cirrhosis and anti-fatigue. Background technique [0002] In today's society, the pace of life is fast, the competition is fierce, and the pressure of work, study and life is high. Many people are busy with work, overdraft their health, eat irregularly, and eat without restraint, causing disorders of the liver and kidney systems, directly or indirectly damaging the liver and kidney. Decreased immunity and decreased disease resistance. In my country, about 15% of people are healthy, 15% are unhealthy, and 70% are in a "sub-healthy" state. At present, the scale and severity of the "sub-healthy" population is not optimistic. It is very urgent to research and develop health care products that protect liver and kidney tissues, prevent liver cirrhosis, resist fatigue, enhance immunity, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K2/00A61K38/01A61P1/16A61P39/00
Inventor 谭高琼黎冠宇
Owner 广西自贸区睿创贸易有限公司
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