Construction and application of a bacterial strain overexpressing the catalytic subunit of thermotoga maritima acetolactate synthase
A bacterial acetolactate synthase catalytic subunit, overexpression technology, applied in the field of genetic engineering, can solve the problems of poor stability, limited application of E. coliAHAS, weak catalytic activity, etc., and achieves the effect of strong catalytic activity
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[0045] Thermotoga marine is a special strict anaerobic bacterium that grows in the environment of 55-90 ℃, such as seabed hot springs, near craters, etc. Because this bacterium can survive under high temperature conditions, the various enzymes expressed in its body also have good thermal stability. Therefore, the thermotogae-derived high-temperature-resistant enzymes have become a hot spot in research and development, such as High temperature resistant xylanase, cellobiose phosphorylase and α-glucosidase have been cloned and expressed, and have been widely used in feed industry, paper industry, energy industry, food and medicine and other fields. In the present invention, primers are designed according to the gene sequence of the AHAS catalytic subunit in GenBank, and the genomic DNA of Thermotoga maritima is used as a template to obtain the target enzyme gene through PCR amplification technology, and the full length of the target fragment is 1746bp. The target fragment was co...
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