Method for extracting and purifying total flavonoids from Indocalamus leaves

A purification method and technology of total flavonoids are applied in the fields of food additives, natural product separation, and medicine, and can solve the problems of high one-time cost input, high concentration of ethanol aqueous solution, long reflux extraction time, etc., and achieve less impurities, low ethanol concentration, The effect of high extraction rate

Inactive Publication Date: 2015-12-23
武汉工商学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But ultrasonic method and microwave method need special instrument, and one-time cost input is high; Direct reflux method needs to use the concentration of ethanol aqueous solution higher, maintains temperature height during reflux, reflux extraction time is long, and comprehensive cost is higher, (document " Ruo Ye In the research on the extraction method of total flavonoids", Li Shuifang et al. showed that 85% ethanol solution could be used to reflux at 85°C for 3h to extract the total flavonoids from the leaves of Ruo luteum; The conditions for extracting total flavonoids in the study of Chunhua et al. are: use ethanol concentration of 85%, solid-liquid ratio of 1:20 (g:mL), reflux temperature of 85°C, and extraction time of 50min); Total flavonoids extraction and purification technology has a good market prospect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Ruoye raw materials were washed and dried at 40°C for 5 hours, added to a traditional Chinese medicine grinder, pulverized evenly, and passed through a 20-mesh sieve to obtain Ruoye particles. Put the Ruoye granules into the extraction bottle, add 5 times the amount (mL:g, the multiple relationship between the value when the volume of the solution added is in mL and the value when the used Ruoye quality is in g, this paper The multiple relationship in the patent is all the same) saturated lime aqueous solution for 0.5h, then add 20 times the volume fraction of 50% ethanol aqueous solution to adjust the pH of the system to about 6.5-7, extract at 60°C for 40min, and pump under reduced pressure Filter to obtain the total flavonoid extract. Concentrate the above-mentioned total flavonoid extract on a rotary evaporator under reduced pressure (vacuum degree 0.095Mpa, temperature 50°C) until the remaining liquid in the desolventization bottle is 1 / 3 of the original volume, ad...

Embodiment 2

[0023] Similar to Example 1, but without adding saturated lime water for pre-soaking. Ruoye raw materials were washed and dried at 40°C for 5 hours, added to a traditional Chinese medicine grinder, pulverized evenly, and passed through a 20-mesh sieve to obtain Ruoye particles. Put Ruoye granules into an extraction bottle, add 20 times the volume fraction of 50% ethanol aqueous solution, extract at 60°C for 40 minutes, and filter under reduced pressure to obtain the total flavonoid extract. Concentrate the above-mentioned total flavonoid extract on a rotary evaporator under reduced pressure (vacuum degree 0.095Mpa, temperature 50°C) until the remaining liquid in the desolventization bottle is 1 / 3 of the original volume, add HPD800 resin column for adsorption, and load the sample Flow rate 2BV / h, after adsorption saturation, elution with 5BV column volume of water, elution with 70% ethanol aqueous solution, collecting eluent and concentrating, vacuum freeze-drying of concentrat...

Embodiment 3

[0025] Ruoye raw materials were washed and dried at 40°C for 5 hours, added to a traditional Chinese medicine grinder, pulverized evenly, and passed through a 20-mesh sieve to obtain Ruoye particles. Put the Ruoye granules into the extraction bottle, add 2 times the amount of saturated lime aqueous solution to pre-soak for 1 hour. Then add 20 times the volume fraction of 50% ethanol aqueous solution to adjust the pH of the system to about 6.5-7, extract at 70° C. for 40 minutes, and filter under reduced pressure to obtain the total flavonoid extract. Concentrate the above-mentioned total flavonoid extract on a rotary evaporator under reduced pressure (vacuum degree 0.095Mpa, temperature 50°C) until the remaining liquid in the desolventization bottle is 1 / 3 of the original volume, add HPD800 resin column for adsorption, and load the sample Flow rate 2BV / h, after adsorption saturation, elute with 5BV column volume of water, elute with ethanol aqueous solution with a volume fract...

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Abstract

The invention belongs to the technical field of natural product separation, food additives and medicines, and in particular relates to a method for extracting and purifying total flavonoids from Indocalamus leaves. The method comprises the following steps: a, washing, drying and crushing Indocalamus leaves; b, presoaking the crushed Indocalamus leaves for 20-60min by using saturated lime water, adding ethanol aqueous solution, then adjusting the acidity and alkalinity of the system to be nearly neutral, performing heating extracting for more than or equal to 30min by controlling the heating extraction temperature to be 50-70 DEG C, and then performing vacuum suction filtration to obtain total a flavonoid extract; c, performing evaporating concentration on the total flavonoid extract, adsorbing by using macroporous resin, washing with water, and then eluting with ethanol aqueous solution to obtain eluent; and d, performing evaporating concentration on the eluent, and performing freeze drying to obtain the total flavonoids. The method provided by the invention has the beneficial effects that the yield of the total flavonoids is relatively high, the concentration of the ethanol solution used during extraction is relatively low, and the temperature during extraction is relatively low.

Description

technical field [0001] The invention belongs to the technical fields of separation of natural products, food additives and medicine, and in particular relates to a method for extracting and purifying total flavonoids from leaves of rutabaga. Background technique [0002] Modern studies have shown that total flavonoids have various biological activities such as anti-tumor, anti-inflammatory, anti-bacterial, anti-atherosclerosis, hypolipidemic, immune regulation, scavenging free radicals, etc., especially for tumor occurrence, proliferation, migration, angiogenesis and multiple Drug resistance has a significant inhibitory effect. In addition, total flavonoids have low toxicity or even no toxicity to normal cells of the human body, so total flavonoids have broad application prospects in human health care, nutrition, and disease prevention and treatment. [0003] Ruoye is the general term for the leaves of the genus Ruozhu in the Poaceae Bamboo subfamily. There are more than 30...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/899A23L1/30
Inventor 乐薇吴士筠杨文婷柯斌清覃瑞
Owner 武汉工商学院
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