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Method for promoting volatile chlorinated hydrocarbons to reduce and decompose through electrical stimulation

A technology of volatile and chlorinated hydrocarbons, applied in the direction of using microorganisms, etc., can solve the problems of slow degradation, toxic and harmful intermediate products, incomplete decomposition, etc.

Inactive Publication Date: 2015-12-30
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the technical problems of the existing anaerobic biological reduction method of volatile chlorinated aliphatic hydrocarbons, such as slow degradation speed, incomplete decomposition, and easy production of toxic and harmful intermediate products, and provides an electric stimulation to promote the anaerobic reduction of volatile chlorinated aliphatic hydrocarbons. Oxybioreductive Decomposition Method

Method used

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  • Method for promoting volatile chlorinated hydrocarbons to reduce and decompose through electrical stimulation
  • Method for promoting volatile chlorinated hydrocarbons to reduce and decompose through electrical stimulation

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specific Embodiment approach 1

[0015] Embodiment 1: A method of electrical stimulation to promote the biological reduction and decomposition of volatile chlorinated hydrocarbons in this embodiment is carried out according to the following steps:

[0016] 1. Preparation of culture medium: add 50mL of distilled water to 10-15mL of activated sludge, add distilled water and activated sludge into a glass bottle, blow nitrogen for 15-20 minutes, and then seal it to maintain anaerobic conditions in the bottle and obtain culture base;

[0017] 2. Enrichment and strengthening of functional flora: Add sodium acetate solution and volatile chlorinated aliphatic hydrocarbons to the medium, place the medium in a constant temperature incubator at 30°C, and measure volatile chlorine every 3 to 5 days Degradation of substituted aliphatic hydrocarbons; when the content of volatile chlorinated aliphatic hydrocarbons drops below 50% of the initial concentration, add sodium acetate solution and volatile chlorinated aliphatic hy...

specific Embodiment approach 2

[0022] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the volatile chlorinated aliphatic hydrocarbon in step 2 is trichloroethylene, tetrachloroethylene or 1,2-dichloroethane; the others are the same as Embodiment 1 .

specific Embodiment approach 3

[0023] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the composition of the anaerobic culture liquid described in step three is as follows: according to the ratio preparation that every 1000mL anaerobic culture liquid comprises the following components: 11.55gNa 2 HPO 4 12H 2 O, 2.77gNaH 2 PO 4 2H 2 O, 0.31gNH 4 Cl, 0.13gKCl, 0.41gCH 3COONa, 0.1mL vitamin solution, 0.1mL mineral element solution and the rest of distilled water; wherein the vitamin solution contains 2.0mg vitamin H, 2.0mg folic acid, 10.0mg pyridoxine hydrochloride, 5.0mg thiamine per 1000mL vitamin solution 5.0mg riboflavin, 5.0mg nicotinic acid, 5.0mg D-calcium pantothenate, 0.1mg vitamin B12, 5.0mg p-aminobenzoic acid, 5.0mg lipoic acid and the rest of distilled water; the mineral element solution is per 1000mL Mineral element solution contains 1.5g nitrilotriacetic acid, 3.0g MgSO 4 ·7H 2 O, 0.5gMnSO 4 ·H 2 O, 1.0gNaCl, 0.1gFeSO 4 ·7H 2 ...

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Abstract

The invention discloses a method for promoting volatile chlorinated hydrocarbons to reduce and decompose through electrical stimulation, which relates to a method for promoting volatile chlorinated hydrocarbons to degrade, and aims to solve the technical problems that existing methods for reducing anaerobic organisms of volatile chlorinated hydrocarbons are slow in degradation velocity, not complete to decompose and easy to produce poisonous and harmful intermediates. The method for promoting the volatile chlorinated hydrocarbons to reduce and decompose through the electrical stimulation comprises steps: 1 preparation of culture medium; 2 enrichment and reinforcement of functional floras; 3 configuration of anaerobic culture; 4 purification of enriched bacterial liquid; 5 construction and start of a biological electrochemical reactor system; and 6 operation of the biological electrochemical reactor system. The method for promoting the volatile chlorinated hydrocarbons to reduce and decompose through the electrical stimulation is a biological method by utilizing a bioelectrochemistry system to promote microorganisms to reduce volatile chlorinated hydrocarbons by adding voltage stimulation. The method for promoting the volatile chlorinated hydrocarbons to reduce and decompose through the electrical stimulation can achieve degradation rate above 95% in 24h, can reduce pollutants containing the volatile chlorinated hydrocarbons, and is used in environmental management engineering.

Description

technical field [0001] The present invention relates to a method for promoting the reductive decomposition of volatile chlorinated hydrocarbons (VCHs). technical background [0002] Volatile chlorinated hydrocarbons (VCHs) are a class of extremely harmful and refractory organic pollutants. They are characterized by volatility, high toxicity, high density, and high solubility. They have the risk of teratogenic, carcinogenic and mutagenic. can accumulate in the environment and cause serious damage to ecosystems and human health. With the wide application of volatile chlorinated aliphatic hydrocarbons in leather, dry cleaning, pesticides and other fields, the detection rate of such pollutants in soil and groundwater is extremely high. At present, many types of VCHs are listed by China and the US Environmental Protection Agency as Priority control of pollutant blacklist. [0003] In 2012, Xu Shuyuan's master's thesis "Experimental Research on the Removal of Volatile Chlorinate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A62D3/02C12N1/36A62D101/22
Inventor 李智灵杨佳琦王爱杰南军孙凯王黎任昇
Owner HARBIN INST OF TECH
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