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A method of electrical stimulation to promote the bioreductive decomposition of volatile chlorinated hydrocarbons

A technology of volatile and chlorinated hydrocarbons, applied in the direction of using microorganisms, etc., can solve the problems of slow degradation, incomplete decomposition, toxic and harmful intermediate products, etc.

Inactive Publication Date: 2018-05-18
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the technical problems of the existing anaerobic biological reduction method of volatile chlorinated aliphatic hydrocarbons, such as slow degradation speed, incomplete decomposition, and easy production of toxic and harmful intermediate products, and provides an electric stimulation to promote the anaerobic reduction of volatile chlorinated aliphatic hydrocarbons. Oxybioreductive Decomposition Method

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  • A method of electrical stimulation to promote the bioreductive decomposition of volatile chlorinated hydrocarbons
  • A method of electrical stimulation to promote the bioreductive decomposition of volatile chlorinated hydrocarbons

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specific Embodiment approach 1

[0015] Specific implementation mode 1: The method for promoting the bioreductive decomposition of volatile chlorinated hydrocarbons by electrical stimulation according to this embodiment is carried out in the following steps:

[0016] 1. The preparation of the culture medium: add the distilled water and the activated sludge to the glass bottle at the ratio of 50mL of distilled water to 10-15mL of activated sludge. After blowing nitrogen for 15~20min, seal it to keep the bottle anaerobic and get the culture base;

[0017] 2. Enrichment and enhancement of functional flora: add sodium acetate solution and volatile chlorinated aliphatic hydrocarbons to the culture medium, place the culture medium in a constant temperature incubator at 30°C, and measure volatile chlorine every 3 to 5 days Degradation of substituted aliphatic hydrocarbons; when the content of volatile chlorinated aliphatic hydrocarbons drops below 50% of the initial concentration, add sodium acetate solution and volatile...

specific Embodiment approach 2

[0022] Specific embodiment two: this embodiment is different from specific embodiment one in that the volatile chlorinated aliphatic hydrocarbon in step two is trichloroethylene, tetrachloroethylene or 1,2-dichloroethane; the others are the same as specific embodiment one .

specific Embodiment approach 3

[0023] Specific embodiment three: This embodiment is different from specific embodiment one or two in that: the composition of the anaerobic culture solution described in step three is as follows: it is prepared at a ratio of the following components per 1000mL anaerobic culture solution: 11.55gNa 2 HPO 4 ·12H 2 O, 2.77g NaH 2 PO 4 ·2H 2 O, 0.31g NH 4 Cl, 0.13g KCl, 0.41g CH 3 COONa, 0.1mL vitamin solution, 0.1mL mineral element solution and the remainder of distilled water; wherein the vitamin solution contains 2.0mg vitamin H, 2.0mg folic acid, 10.0mg pyridoxine hydrochloride, 5.0mg thiamine per 1000mL vitamin solution Element, 5.0mg riboflavin, 5.0mg niacin, 5.0mg calcium D-pantothenate, 0.1mg vitamin B12, 5.0mg p-aminobenzoic acid, 5.0mg lipoic acid and the remainder of distilled water; the mineral element liquid is per 1000mL mineral element liquid contains 1.5g nitrilotriacetic acid, 3.0g MgSO 4 ·7H 2 O, 0.5gMnSO 4 ·H 2 O, 1.0g NaCl, 0.1g FeSO 4 ·7H 2 O, 0.1g CoCl 2 ·6H 2 O...

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Abstract

The invention relates to a method for promoting the biological reduction and decomposition of volatile chlorinated hydrocarbons by electrical stimulation, and the invention relates to a method for promoting the degradation of volatile chlorinated hydrocarbons. The invention aims to solve the technical problems that the existing anaerobic biological reduction method of volatile chlorinated aliphatic hydrocarbons has slow degradation speed, incomplete decomposition and easy production of toxic and harmful intermediate products. The method: 1. Preparation of culture medium; 2. Enrichment and strengthening of functional flora; 3. Configuration of anaerobic culture solution; 4. Purification and enrichment of bacteria solution; 6. The operation of the bioelectrochemical system reactor. The present invention is a biological method that uses a bioelectrochemical system to stimulate microorganisms to reduce volatile chlorinated hydrocarbons through external voltage stimulation. The degradation rate of the method in the present invention can reach more than 95% in 24 hours, and can degrade fats containing volatile chlorinated hydrocarbons. Hydrocarbon pollutants are used in environmental governance projects.

Description

Technical field [0001] The invention relates to a method for promoting the reductive decomposition of volatile chlorinated hydrocarbons (VCHs). technical background [0002] Volatile chlorinated hydrocarbons (VCHs) are a class of extremely hazardous and refractory organic pollutants. They are volatile, highly toxic, high-density, high-solubility, etc., and have the risk of teratogenic, carcinogenic, and mutagenic. It can accumulate continuously in the environment and cause serious harm to the ecosystem and human health. With the widespread application of volatile chlorinated aliphatic hydrocarbons in tanning, dry cleaning, pesticides and other fields, the detection rate of such pollutants in soil and groundwater is extremely high. At present, many types of VCHs are listed by China and the US Environmental Protection Agency as Prioritize control of pollutant blacklists. [0003] In 2012, Xu Shuyuan's master's thesis "Experimental Research on the Removal of Volatile Chlorinated Hyd...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A62D3/02C12N1/36A62D101/22
Inventor 李智灵杨佳琦王爱杰南军孙凯王黎任昇
Owner HARBIN INST OF TECH
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