Lycium rethenicum Murr polysaccharide extract and preparation method thereof

A technology of black fruit wolfberry and wolfberry polysaccharide, which is applied in drug combination, metabolic diseases, digestive system and other directions, can solve the problems of destroying polysaccharide structure, complicated extraction process, and excessive extraction time, and achieves simplified extraction process and simple operation. Effect

Active Publication Date: 2016-01-06
XINING ZHIYE BIOTECH CONSULTING SERVICE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have many problems, such as destroying the polysaccharide structure, low polysaccharide extraction efficiency, long extraction time and complicated extraction process, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Preparation of black fruit Lycium barbarum polysaccharide

[0046] 1. Cleaning: sieve 1kg of black fruit wolfberry to remove the sediment and wash it with water;

[0047] 2. Drying: Dry the cleaned Lycium barbarum at 30℃ for 10h at low temperature;

[0048] 3. Crushing: Ultrafine crushing is performed on the Lycium barbarum black fruit obtained in step 2 to obtain a powder with a particle size range of 35 μm;

[0049] 4. Removal of impurities: the powder obtained in step 3 is placed in a continuous microwave reactor under 500W power, and extracted with diethyl ether for 25 minutes to obtain medicinal residues I; II is extracted with ethanol reflux to obtain the medicinal residue III; the medicinal residue III is evaporated to dry the solvent;

[0050] 5. Extraction: add 9 times of water to the obtained medicinal residue III, place it in the ultrasonic reflux extraction system, extract 1.5h by ultrasonic reflux, ultrasonic power 1.5KW, extraction temperature...

Embodiment 2

[0056] Example 2: Preparation of black fruit Lycium barbarum polysaccharide

[0057] 1. Cleaning: sieve 1kg of black fruit wolfberry to remove the sediment and wash it with water;

[0058] 2. Drying: Dry the cleaned Lycium barbarum at 20℃ for 10h at low temperature;

[0059] 3. Crushing: ultrafinely crushing the Lycium nigra obtained in step 2 to obtain a powder with a particle size range of 30 μm;

[0060] 4. Removal of impurities: the powder obtained in step 3 is placed in a continuous microwave reactor under 450W power, and extracted with ether for 20 minutes to obtain medicinal residues I; II was extracted with ethanol under reflux to obtain drug residue III; drug residue III was evaporated to dryness.

[0061] 5. Extraction: add 10 times the amount of water to the obtained medicinal residue III, place it in an ultrasonic reflux extraction system, extract by ultrasonic reflux for 1 h, ultrasonic power 0.5KW, and extraction temperature of 40° to obtain filtrate I.

[006...

Embodiment 3

[0068] Determination of Polysaccharide Content Purified by Different Macroporous Resin Types by Phenol-Sulfuric Acid Colorimetry

[0069] 1 Preparation of phenol solution

[0070] Take 200 g of phenol, add 0.2 g of aluminum flakes and 0.1 g of sodium bicarbonate for distillation, and collect fractions at 182°C. Weigh 5g, add distilled water to 100mL to dissolve, mix and transfer to a brown bottle, and store in a refrigerator for later use.

[0071] 2 Preparation of standard solutions

[0072] Accurately weigh 100.00 mg of analytically pure glucose dried at 105 °C to constant weight, dissolve in distilled water and dilute to volume in a 100 mL volumetric flask to obtain a 1 mg / mL standard solution, pipette 2 mL, 4 mL, 6 mL, 8 mL, and 10 mL, respectively, The standard sugar solutions of 20 μg / mL, 40 μg / mL, 60 μg / mL, 80 μg / mL and 100 μg / mL were obtained by diluting to volume in a 100 mL volumetric flask.

[0073] 3 Drawing of the standard curve

[0074] Take 2 mL of standard ...

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Abstract

The invention relates to a Lycium rethenicum Murr polysaccharide extract and a preparation method thereof. Lycium rethenicum Murr polysaccharide obtained by preparation method of cleaning, drying, breaking, removing impurities, extracting, decoloring, deproteinizing, condensing, purifying and the like has the efficacies of nourishing yin and tonifying kidney, improving eyesight and nourishing liver, reducing fat, resisting cancer, resisting aging and the like; meanwhile, the preparation method of the Lycium rethenicum Murr polysaccharide has the advantages of simple process, easiness in operation, higher yield and good quality, and facilitates the realization of industrial production and processing.

Description

technical field [0001] The invention relates to the field of plant extracts, in particular to a polysaccharide extract of Lycium barbarum nigra with functions of nourishing yin and strengthening kidney, improving eyesight and nourishing liver, reducing lipid, preventing cancer, anti-aging and the like, and a preparation method thereof. Background technique [0002] Lycium ruthenicum is the dry fruit of Lycium Ruthenicum Murr, which belongs to the Solanaceae family. It is scattered in Jiuquan, Gansu, eastern Qinghai, central Xinjiang, western Inner Mongolia, Ningxia, and Tibet in China. It grows in the barren mountains and riverbeds and beaches where human beings cannot survive. It has strong vitality. The wild black fruit wolfberry in Golmud, Qinghai is known as the "soft gold" of the grassland. However, with the development of nature by human beings, the living space of wolfberry is less and less, creating a rare and precious original ecological high-end tonic. It is sweet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P13/12A61P27/02A61P3/06A61P35/00A61P1/16
Inventor 王玉花刘洪智马菊秀
Owner XINING ZHIYE BIOTECH CONSULTING SERVICE
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