A method for enhancing microbial attachment effect in chalcopyrite bioleaching process

A technology of bioleaching and ore leaching microorganisms, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems that leaching microorganisms have not been well studied and the leaching system is complex, and achieve enhanced leaching microorganism adhesion effect, improving leaching efficiency, and improving adaptability

Active Publication Date: 2019-03-19
JIANGNAN UNIV
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  • Description
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  • Application Information

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Problems solved by technology

However, due to the special biological characteristics of this type of leaching microorganisms and the complexity of the leaching system, there is still no good research on how to effectively strengthen the attachment effect of leaching microorganisms.

Method used

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  • A method for enhancing microbial attachment effect in chalcopyrite bioleaching process
  • A method for enhancing microbial attachment effect in chalcopyrite bioleaching process
  • A method for enhancing microbial attachment effect in chalcopyrite bioleaching process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The determination of the overall bacterial cell concentration in the adsorbed state in the leaching process of different bacterial species chalcopyrite ore of embodiment 1

[0027] The chalcopyrite sample (containing 1.0% copper) comes from the Tongling Mountain mining area in Anhui Province. After preliminary crushing and filtering with a steel sieve, the ore particle size is 7 individual / mL, (NH 4 ) 2 SO 4 3.0g / L, K 2 HPO 4 0.5g / L, MgSO 4 ·7H 2 O 0.5g / L, KCl0.1g / L, Ca(NO 3 ) 2 0.01g / L. Thiobacillus thiooxidans ZJJN system: 100mL Starkey medium, cell concentration 5.0×10 7 pieces / ml. (NH 4 ) 2 SO 4 3.0g / L, KH 2 PO 4 3.5g / L, MgSO 4 0.5g / L, CaCl 2 2H 2 O 0.25g / L. The slurry concentration is 3.0%, the temperature is 30° C., and the shaking table rotates at 170 rpm.

[0028]The leaching process and the attached cell washing treatment steps are as follows. After 2 weeks of leaching, the leaching solution was clarified for 1 hour, the supernatant was remo...

Embodiment 2

[0032] Example 2 Comparison of the morphology of leached slag before and after treatment with enhanced microbial adhesion effect

[0033] In order to better understand the effect of attached cells after strengthening treatment, the morphology difference of slag before and after strengthening was observed by scanning electron microscope ( figure 2 ). In the system of Thiobacillus ferrooxidans, the slag surface of the untreated system is relatively smoother. However, the surface of slag in the enhanced leaching system is rougher, and there are more traces of adhesion, such as micro pits and ravines. At the same time, it also implies that under the enhanced attachment effect, the metabolism of iron ions is accelerated, and more jarosite precipitation appears on the ore surface. A similar phenomenon also exists in the system of Thiobacillus thiooxidans. With the enhanced adhesion of the attached cells, the "contact" mechanism is strengthened, especially the energy material par...

Embodiment 3

[0034] Embodiment 3 enhances the comparison of leaching efficiency before and after the treatment of microbial adhesion effect

[0035] Table 1 shows the comparison results of leaching efficiency before and after treatment with enhanced microbial attachment effect. In the system of Thiobacillus ferrooxidans CUMT-1, the final copper ion concentration is 34.4 mg / L, and the copper ion concentration after adopting the method of the present invention domesticated is significantly increased to 46.4 mg / L; in the system of Thiobacillus thiooxidans ZJJN , the final copper ion concentration was 35.2mg / L, and the copper ion concentration after domestication was significantly increased to 52.2mg / L. In addition, it has a similar effect on Thiobacillus ferrooxidans ATCC23270.

[0036] Table 1 Comparison of leaching efficiency before and after treatment with enhanced microbial attachment effect

[0037]

[0038] The results show that through the long-term washing treatment and selection...

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Abstract

The invention discloses a method for enhancing the microorganism adhesion effect in the bioleaching process of chalcopyrite, and belongs to the technical field of bioleaching. The method comprises the steps that stimulus intensity washing acclimatization is conducted on cells adherent to ore surfaces, different bioleaching systems such as a pure thelloobacillus ferrooxidans system and a pure thiobacillus thiooxidans system which are leached through the chalcopyrite are verified, and it is found that the method can efficiently enhance the microorganism adhesion effect in the bioleaching process; operation is easy, and the method is suitable for large-scale application and promotion.

Description

technical field [0001] The invention relates to a method for enhancing the microbial attachment effect in the bioleaching process of chalcopyrite, and belongs to the technical field of bioleaching. Background technique [0002] Bioleaching, also known as biooxidation or biohydrometallurgy, is an interdisciplinary subject in the fields of biology and minerals. It uses microorganisms as the medium and leachate as the carrier to promote the dissolution of minerals through a series of oxidation-reduction reactions, and combined with the subsequent electrowinning-extraction process to achieve the maximum mining and utilization of minerals. Compared with the traditional high-temperature smelting process, this method has low infrastructure investment costs, mild operating conditions, recyclable leaching waste liquid, and effective recovery of low-grade minerals. It is recognized as an alternative green metallurgical technology. [0003] Chalcopyrite belongs to sulfide ore and is t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/14C22B3/18C22B15/00C12R1/01
CPCY02P10/20
Inventor 冯守帅杨海麟辛瑜夏小乐张玲詹晓方政
Owner JIANGNAN UNIV
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