Method for simultaneously detecting multiple drug residues in poultry tissues

Abstract

The invention discloses a method for detecting drug residues in poultry tissues. The method comprises the following steps of selecting a poultry sample, adding sodium chloride and acetonitrile, shaking out, adding sodium hydroxide solution, and vibrating for 30s to 5min; then, ultrasonic extracting for 10 to 40min, centrifuging, absorbing supernate for standby use, drying other solution by distillation, adding concentrated hydrochloric acid in the residues, adjusting pH to be about 1 to 3; then, adding acetonitrile, vibration extracting, centrifuging solution subjected to acetonitrile vibration extracting, absorbing supernate to merge with the above supernate, concentrating to dry to obtain sample extracting solution residues; adding a matrix solid-phase dispersion agent and 2ml ethyl acetate, swirling for 2min, taking supernatant liquid, drying through nitrogen, then adding 30 percent of methanol solution containing 0.1 percent of methanoic acid, ultrasonic dissolving, filming and injecting the sample; adopting HLPC-MS to carry out gradient elution detection. According to the method provided by the invention, the current standard one-time one-sample one-item is adjusted to one-time one-sample five-items, so that the time consumed for detection is greatly shortened.

Description

technical field [0001] The invention relates to a chromatographic detection method, in particular to a method for detecting drug residues by ultra-high performance liquid chromatography tandem mass spectrometry, and belongs to the field of chromatographic analysis. Background technique [0002] Poultry meat generally refers to chicken, duck, goose, etc. It is famous for its high protein, low fat, and high nutrition, and plays an important role in people's daily life. Most of the poultry meat sold on the market is large-scale breeding. Due to the high density of poultry gathering in the large-scale breeding process, once a poultry gets sick, it will spread quickly and epidemic diseases will appear. Therefore, general farmers will add an appropriate amount of drugs during the breeding process to prevent the emergence of diseases. Correspondingly, there are clear requirements for the type and time of drug use in the national standard. [0003] In order to increase the breeding...

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to overcome the defects of outdated and non-uniform standards for drug residue detection methods in the prior art, and provide a detection method for multiple drug residues in poultry meat tissue

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