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Preparation method of magnetic core-shell ionic liquid immobilized lipase and its application in edible oil processing

A technology for immobilizing lipase and ionic liquid, applied in the direction of immobilization on or in inorganic carrier, immobilization on/in organic carrier, sustainable manufacturing/processing, etc., can solve the use limitation, increase cardiovascular and cerebrovascular Disease risk and other issues, to achieve the effect of increasing loading, good catalytic activity and specificity, and improving adaptability

Active Publication Date: 2018-07-06
唐河金海生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the trans fatty acids in hydrogenated fats can significantly increase the risk of cardiovascular and cerebrovascular diseases, its use is gradually limited

Method used

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  • Preparation method of magnetic core-shell ionic liquid immobilized lipase and its application in edible oil processing
  • Preparation method of magnetic core-shell ionic liquid immobilized lipase and its application in edible oil processing
  • Preparation method of magnetic core-shell ionic liquid immobilized lipase and its application in edible oil processing

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preparation example Construction

[0034] Such as figure 1 Shown, the preparation method of magnetic core-shell type ionic liquid immobilized lipase of the present invention comprises the following steps:

[0035] First step, Fe 3 o 4 Preparation of @MCM-41 core-shell composites

[0036] Firstly, Fe was prepared by chemical co-precipitation method 3 o 4 Nanoparticles:

[0037] FeCl 3 ·6H 2 O, FeSO 4 ·7H 2 O. Add distilled water into a three-neck round bottom flask, stir it mechanically to dissolve it completely and mix it evenly, then slowly add an appropriate amount of ammonia water to adjust the pH of the solution to 10, and a large amount of black precipitates - Fe 3 o 4 Nanoparticles, continue to stir and react for 1 h, then transfer the solution to a beaker, wash with distilled water, absolute ethanol, and trisodium citrate solution, and then wash the Fe 3 o 4 Nanoparticles are magnetically separated under the action of an external magnetic field, and vacuum-dried at 60°C, ground and stored in ...

Embodiment 1

[0052] (1) Fe 3 o 4 Preparation of nanoparticles

[0053] Fe 3 o 4 Nanoparticles were prepared by chemical co-precipitation method. Weigh 8.1g FeCl 3 ·6H 2 O, 3.9g FeSO 4 ·7H 2 0 and 150ml of distilled water were added to a 250ml three-necked round-bottomed flask, mechanically stirred to make it completely dissolved and uniformly mixed, then slowly added an appropriate amount of ammonia water, and the pH of the solution was adjusted to be 10, and a large amount of Fe was quickly generated in the mixed solution. 3 o 4 Nanoparticles (black precipitate), continue to stir and react for 1h, transfer the solution to a 250ml beaker, wash with distilled water, absolute ethanol, and 0.3mol / L trisodium citrate solution for 3 times, and then wash the Fe 3 o 4 Nanoparticles are magnetically separated under the action of an external magnetic field, and vacuum-dried at 60°C, ground and stored in a centrifuge tube for later use;

[0054] (2) Fe 3 o 4 Preparation of @MCM-41 Compo...

Embodiment 2

[0065] Fe 3 o 4 Nanoparticles and Fe 3 o 4 The preparation of @MCM-41 composite material is the same as in Example 1.

[0066] Functional modification of the carrier: 20 mmol of methylimidazole and 20 mmol of trichloropropyltriethoxysilane were added into a 250 mL three-necked flask, and stirred and reacted at 95° C. for 26 h under nitrogen protection. Then, 1.0g Fe 3 o 4 The @MCM-41 composite material was added to the above system, and 100mL of ethanol was added as a solvent, protected by nitrogen, and refluxed at 90°C for 24h. After the reaction, the product was magnetically separated, washed several times with absolute ethanol and ether, and vacuum-dried at 40°C to obtain the ionic liquid-modified carrier IL-Fe 3 o 4 @MCM-41;

[0067] Immobilization of lipase: Disperse 2.0 g of free lipase in 30 mL of phosphate buffer solution (0.2 mol / L, pH=7.0), and then add 1.0 g of the above-mentioned ionic liquid modified magnetic composite carrier IL-Fe 3 o 4 @MCM-41, the mi...

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Abstract

The invention discloses a magnetic core-shell type ionic liquid immobilized lipase preparation method. The preparation method includes the steps that firstly, a chemical coprecipitation method is adopted for preparing Fe<3>O<4> nanometer particles; then, the prepared Fe<3>O<4> nanometer particles serve as magnetic cores for synthesizing a Fe<3>O<4>@MCM-41 mesoporous composite material of a core-shell structure; a magnetic carrier is subjected to ionic liquid functionalized modification; finally, immobilization of the lipase is conducted by the adoption of a mesoporous core-shell type magnetic nanometer microsphere carrier modified by ion liquid. According to the magnetic core-shell type ionic liquid immobilized lipase preparation method, the magnetic carrier is modified through the ion liquid, the adaptability of the lipase to the carrier is improved, meanwhile the load of the lipase is increased, and the activity and stability of the lipase are improved; the prepared magnetic immobilized enzyme can be used for a grease ester-ester exchange reaction system with large viscosity, and the magnetic immobilized enzyme can be used for preparing modified grease in the edible oil processing industry.

Description

technical field [0001] The invention relates to a lipase catalyst, in particular to a preparation method of a magnetic core-shell type ionic liquid immobilized lipase, and also relates to the application of the prepared lipase in edible oil processing. Background technique [0002] Lipase is a biological macromolecule with biocatalytic function. Compared with chemical catalysts, it has the advantages of mild reaction conditions, high catalytic efficiency, strong specificity, and less side reactions. It is widely used in medicine, chemical industry, food, light industry and Environmental protection and other industries. However, free lipase has poor operational stability, is easily inactivated, and is not easy to be reused. It is difficult to realize the continuous and automatic production process, and it is difficult to separate from the reaction system after the reaction. Although the catalysis technology of free lipase is relatively mature, the real industrial application...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/08C12P7/64
CPCY02P20/50
Inventor 谢文磊臧雪珍张弛王宏雁
Owner 唐河金海生物科技有限公司
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