Centrifugal gas-electric spinning device by utilizing negative-pressure array

Abstract

The invention discloses a centrifugal gas-electric spinning device by utilizing a negative-pressure array. The centrifugal gas-electric spinning device comprises a high-voltage alternating-current generator, an air supply device, a box body, a centrifugal sprayer, a centrifugal driving mechanism and negative-pressure receiving devices. The interior of the centrifugal sprayer is provided with a liquid storage cavity. Filament outlet holes are arranged in the centrifugal sprayer. Each negative-pressure receiving device comprises a collector, a negative-pressure pipe and a negative air-pressure generator. Negative-pressure pipe openings are formed by negative-pressure pipes in the bottom of the box body. Collectors are nested into the negative-pressure pipe openings. The multiple negative-pressure receiving devices are arrayed in an annular array with the centrifugal sprayer as the center. The high-voltage alternating-current generator is formed for generating an electric field. Airflow spraying out of the filament outlet holes is formed by the air supply device. The centrifugal gas-electric spinning device by utilizing the negative-pressure array has following beneficial effects: nanofibers are formed by sprayed polymers under airflow, an electric field, negative pressure and centrifugal force such that a three-dimensional structure is formed by nanofibers on the negative-pressure receiving devices; thickness of fiber disposition is increased; biological materials are better compatible and the application scope of technology materials is broad; and a support structure obtained is beneficial for cell growth of tissue engineering.

Description

technical field [0001] The invention is used in the technical field of nanofiber supports, and in particular relates to a negative pressure array centrifugal gas electrospinning device. Background technique [0002] Electrospinning is an effective method for preparing nanofibers. The fiber diameter is usually between tens of nanometers and several micrometers, which is similar to the size of fibrous scaffolds such as collagen in the extracellular matrix. The use of natural polymer or synthetic polymer electrospun fibers to construct tissue engineering scaffolds can mimic the structure and even biological functions of extracellular matrix, facilitate cell adhesion, differentiation and proliferation, guide tissue regeneration and repair, and become the research of tissue engineering scaffolds hotspot. A large number of research reports have shown that electrospun nanofibrous scaffolds can provide an ideal microenvironment for cell adhesion, proliferation, and differentiation...

AI Technical Summary

Problems solved by technology

However, the fibers of the scaffold are piled up by the attraction of electric charge, the arrangement is tight, and the gap between the fibers is too small, which makes it difficult for cells to grow in, and it is impossible to construct a satisfactory three-dimensional tissue.

On the other hand, in traditional DC electrospinning, the direction of the electric field force is constant. When the viscosity of the spinning solution or melt is high, the starting voltage required by the jet is high.

[0006] 2. As the thickness of the film support increases, the electric field between the nozzle and the collecting plate will gradually weaken, resulting in the continuous accumulation of surface charges on the film support. Because of the same charge, the first deposited fibers will affect the electric field distribution and affect the subsequent jet flow. Repulsion occurs, which affects the formation of the Taylor cone and the duration of spinning, reduces the spinning efficiency, and eventually leads to the termination of the electrospinning process, which limits the thickness of the scaffold that can be obtained (usually micron-scale thickness), and the real fabric of large thickness Significant electrospun three-dimensional structures remain difficult

[0007] 3. When a collector with a specific shape is used, the fibers are deposited toward the tip where the electric field strength is higher, making it difficult to form a three-dimensional structure

[0008] 4. The efficiency of traditional nozzle-type electrospinning is low, and the output of single-nozzle electrospinning is usually only 0.1-1g / h, which makes it difficult to realize large-scale and high-efficiency three-dimensional scaffold manufacturing

However, the scheme of using negative pressure to collect AC air electrospun nanofibers to prepare three-dimensional scaffolds has not been reported yet.

[0012] In summary, there is still no universal solution for preparing nanofibrous three-dimensional scaffolds with a wide range of materials, extremely small fiber diameters, uniform filament diameter distribution, fluffy structure, extremely thick, and superior mechanical properties.

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