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Making method of molecularly imprinted electrochemical sensing electrode for detecting propylene chorohydrin

A molecular imprinting and sensing electrode technology, applied in the direction of electrochemical variables of materials, etc., can solve the problems of high detection cost, inability to quickly monitor the content of chloropropanol in seasonings in real time, and long detection time.

Inactive Publication Date: 2016-02-24
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, chromatography, mass spectrometry and other detection methods are expensive to detect, take a long time to detect and require professional personnel to operate, and cannot perform real-time and rapid monitoring of the content of chloropropanol in condiments.

Method used

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  • Making method of molecularly imprinted electrochemical sensing electrode for detecting propylene chorohydrin
  • Making method of molecularly imprinted electrochemical sensing electrode for detecting propylene chorohydrin

Examples

Experimental program
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Embodiment 1

[0015] Example 1: Synthesis of photocrosslinkable amphiphilic random copolymers

[0016] Weigh a certain amount of DMA, HEA, EHA, St and initiator AIBN into a three-necked flask, add an appropriate amount of ethyl acetate as a solvent, stir evenly at room temperature, and react at 80°C for 20h. AIBN was dissolved in ethyl acetate and added dropwise to the constant temperature reaction system. After the dropwise addition, the temperature of the system was raised to 90° C., and the reaction was continued for 4 hours to obtain the amphiphilic acrylate random copolymer Poly(DMA-co-HEA-co-EHA-co-St).

[0017] Add a certain amount of IPDI and an appropriate amount of solvent ethyl acetate into a three-necked flask equipped with a thermometer, a stirrer and a dropping funnel, add dropwise HEA, stir at room temperature for 2 hours, use DBTDL as a catalyst and DBHT as an initiator, and react at 50°C for 5 hours , to obtain isocyanates with terminal unsaturated bonds.

[0018] Graftin...

Embodiment 2

[0020] Example 2: Preparation of molecularly imprinted micelles

[0021] Dissolve the polymer, template molecule and photoinitiator benzophenone in ethylene glycol butyl ether, and add an appropriate amount of lactic acid to the polymer mixed solution to adjust the pH, and stir to make the template molecule and the polymer fully interact to form a complex things. Then, the precipitant water is slowly dropped into the solution to induce it to form molecularly imprinted polymer micelles, and then it is added into a large amount of water to fix the micelles to obtain an imprinted micellar solution.

Embodiment 3

[0022] Example 3: Preparation of molecularly imprinted electrochemical electrodes

[0023] Polish the bare gold electrode with polishing powder, then ultrasonically clean it with absolute ethanol and water for 5 minutes, and finally rinse it with ultrapure water repeatedly, and dry it for later use. Using the chloropropanol molecularly imprinted micellar solution as the electrodeposition solution, the bare gold electrode was deposited in the deposition solution for 120 s by controlled potential electrolysis (CPE) to prepare the molecularly imprinted polymer micellar film. UV light was applied for 10 min to immobilize the polymer membrane to imprint the acupoints. Finally, the template molecules were eluted with a mixed solution of acetic acid and methanol, and stored in air.

[0024] The fabrication of the non-imprinted electrode is the same as above except that the template molecule chloropropanol is not added.

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Abstract

The invention provides a making method of a molecularly imprinted electrochemical sensing electrode. The method comprises the following steps: self-assembling photo-crosslinkable acrylate-co-styrene random copolymer with propylene chorohydrin as a template molecule through combining macromolecular self-assembling, molecular imprinting and electrophoretic deposition technologies to obtain propylene chorohydrin coated imprinted micelles, depositing through a controlled potential electrolysis technology to form a film on the surface of a gold electrode, carrying out ultraviolet radiation crosslinking on the film under the action of a photoinitiator in order to fix combination sites between propylene chorohydrin and the polymer micelles, and eluting to remove propylene chorohydrin in order to obtain the molecularly imprinted sensing electrode. The sensing electrode can be used to determine the content of propylene chorohydrin, and has good selective identity.

Description

technical field [0001] The invention relates to a preparation method of a molecularly imprinted electrochemical sensing electrode for detecting chloropropanol, which belongs to the technical field of combining functional polymer materials and bionic sensors. Background technique [0002] Chloropropanol is a kind of compound produced by replacing the hydroxyl group of glycerol with chlorine, and its molecular formula is: CH 3 CH(OH)CH 2 Cl, including monochloropropanediol: 3-chloro-1,2-propanediol (abbreviated as 3-chloropropanol, 3-MCPD), 2-chloro-1,3-propanediol (2-MCPD); dichloropropanol: 1 , 3-dichloro-2-propanol (1,3-DCP), 2,3-dichloro-1-propanol (2,3-DCP). Among the chloropropanol series compounds, the main component of contaminated food is 3-MCPD, the secondary component is 1,3-DCP, and the content ratio of the two is 20:1. 3-MCPD is a colorless and transparent liquid, soluble In water, ethanol, ether. The relative molecular weight is 94.54, the relative density is...

Claims

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Application Information

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IPC IPC(8): G01N27/30
Inventor 刘晓亚赵伟黄雪雯肖恬英季磊梁雪赵鑫犇
Owner JIANGNAN UNIV
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