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Polygonatum odoratum polysaccharide sulfation method

A polysaccharide from Polygonatum polysaccharides and sulfated technology, which is applied in the field of sulfated esterification, can solve the problems of unfavorable antiviral activity, toxic side effects, adverse reactions, etc., and achieve the effects of high sulfuric acid substitution degree of the product, simple operation, and easy-to-obtain raw materials

Inactive Publication Date: 2016-03-16
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(2) Some polysaccharides are not conducive to their biological activity due to structural or physical and chemical properties
For example, untreated schizophyllan cannot be used clinically due to its high viscosity; some dextran sulfates cannot cross multiple cell membrane barriers due to their large molecular weight, and it is difficult to reach the blood concentration to exert biological activity
(3) Although some polysaccharides have good medicinal effects, they will also produce some adverse reactions and even toxic side effects
For example, some low-molecular-weight dextran sulfates with antiviral activity can produce anticoagulant phenomena that are not conducive to their antiviral activity; some sulfated derivatives of polysaccharides show certain cytotoxicity due to too many sulfate groups
(4) Some polysaccharides isolated from natural organisms have weak activity and need to be further improved
At present, the research on the sulfation method of polysaccharides from Polygonatum polysaccharides is not mature enough

Method used

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  • Polygonatum odoratum polysaccharide sulfation method
  • Polygonatum odoratum polysaccharide sulfation method
  • Polygonatum odoratum polysaccharide sulfation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 200mg of dried Polygonatum polysaccharide and add it to 30ml of formamide (water has been removed), stir to dissolve, add 800mg of sulfur trioxide pyridine complex, react at 40°C for 5h, and constantly fill with nitrogen during the reaction. After the reaction, the reaction solution was moved to ice water, and 2 mol / L sodium hydroxide was added to adjust the pH of the solution to between 7 and 8. Concentrate the reaction solution to half its original volume, add 4 times the volume of ice-free ethanol, seal it and put it in a 4°C refrigerator for precipitation for 12 hours, collect the precipitate by centrifugation, wash the precipitate with methanol until the precipitate has no pyridine smell. Add a small amount of deionized water to dissolve in the dialysis bag, first dialyze with tap water for 1 to 2 days, then dialyze with deionized water for 2 days, change the water 2 to 3 times a day. The dialysate was concentrated and then freeze-dried to obtain sulfated Poly...

Embodiment 2

[0021] Take 200mg of dried Polygonatum polysaccharide and add it to 30ml of formamide (water has been removed), stir to dissolve, add 800mg of sulfur trioxide pyridine complex, react at 60°C for 4h, and constantly fill with nitrogen during the reaction. After the reaction, the reaction solution was moved to ice water, and 2 mol / L sodium hydroxide was added to adjust the pH of the solution to between 7 and 8. Concentrate the reaction solution to half of the original volume, add 4 times the volume of ice-free ethanol, seal it and put it in a refrigerator at 4°C for precipitation for 12 hours, collect the precipitate by centrifugation, wash the precipitate with methanol until the precipitate has no pyridine smell. Add a small amount of deionized water to dissolve in the dialysis bag, first dialyze with tap water for 1 to 2 days, then dialyze with deionized water for 2 days, change the water 2 to 3 times a day. The dialysate was concentrated and then freeze-dried to obtain sulfate...

Embodiment 3

[0023] Take 200mg of dried Polygonatum polysaccharide and add it to 30ml of formamide (water has been removed), stir to dissolve, add 800mg of sulfur trioxide pyridine complex, react at 80°C for 6h, and constantly fill with nitrogen during the reaction. After the reaction, the reaction solution was moved to ice water, and 2 mol / L sodium hydroxide was added to adjust the pH of the solution to between 7 and 8. Concentrate the reaction solution to half of the original volume, add 4 times the volume of ice-free ethanol, seal it and put it in a refrigerator at 4°C for precipitation for 12 hours, collect the precipitate by centrifugation, wash the precipitate with methanol until the precipitate has no pyridine smell. Add a small amount of deionized water to dissolve in the dialysis bag, first dialyze with tap water for 1 to 2 days, then dialyze with deionized water for 2 days, change the water 2 to 3 times a day. The dialysate was concentrated and then freeze-dried to obtain sulfate...

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Abstract

The present invention belongs to the technical field of sulfation, and specifically discloses a polygonatum odoratum polysaccharide sulfation method. The method comprises: dissolving dried polygonatum odoratum polysaccharide in a reaction solvent, adding a pyridine sulfur trioxide complex, carrying out a reaction for 4-9 h at a temperature of 40-80 DEG C, neutralizing, carrying out ethanol precipitation, carrying out dialysis, concentrating, and drying to obtain the polygonatum odoratum polysaccharide sulfate, wherein a mass volume ratio of the polygonatum odoratum polysaccharide to the reaction solvent is 1:10-1:500 g / ml, and a mass ratio of the polygonatum odoratum polysaccharide to the pyridine sulfur trioxide complex is 1:5-5:1.

Description

technical field [0001] The invention relates to the technical field of sulfation, in particular to a method for sulfation of polygonatum polysaccharide. Background technique [0002] Polysaccharides are a large class of polymer compounds that exist in nature. They are widely involved in various life activities of cells and produce a variety of biological functions, such as enhancing immune characteristics, anti-tumor, anti-virus, anti-coagulation, anti-aging and other functions. With the in-depth research on the composition, properties, structure and function of polysaccharides, people have noticed the following problems: (1) Not all polysaccharides in nature are active. For example, unprocessed pachyranan does not have any biological activity. (2) Some polysaccharides are not conducive to the exertion of their biological activity due to obstacles such as structure or physical and chemical properties. For example, untreated schizophyllan cannot be used clinically due to it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 李钟韩彬王强刘塔斯
Owner GUANGDONG PHARMA UNIV
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