Method for extracting and separating ginkgo flavonoids and ginkgolides from ginkgo leaves

A technology for ginkgo flavonoids and ginkgolides, which is applied in the fields of extracting ginkgoflavones and ginkgolides, and extracting and separating ginkgoflavones and ginkgolides, can solve the problems of low product content, generally low yield and the like, and achieves high content and operation steps. Simple and effective in improving product yield

Active Publication Date: 2020-01-21
SHAANXI JIAHE PHYTOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of them choose water extraction and alcohol precipitation or alcohol extraction and water precipitation, and then use macroporous resin for column chromatography. The content of the product obtained by this method is low, and the general yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Add 10 kg of water (containing 200 g of sodium bicarbonate) into an airtight container, heat to boil, put in 2 kg of fresh ginkgo leaves, and cook for 10 minutes to inactivate the enzyme. Take out the leaves, extract once with 90% ethanol at 70°C, filter, extract the filter residue once with 80% ethanol at 70°C, filter, combine the extracts, concentrate to a specific gravity of 1.1, adjust to neutral, place and filter, and mix the supernatant into 1 / 3 times the amount of polyamide dry. Take another 1.8kg of polyamide and 180g of alumina to pack into the column, use petroleum ether: ethyl acetate = 2:1 to rinse, add the above-mentioned dried sample to the top of the column, and continue to use petroleum ether: ethyl acetate = 2:1 to elute , point the plate for observation, collect the active ingredient segment, after the lactone is eluted, change the ethyl acetate:ethanol=8:1 elution, point the plate for observation, and collect the active ingredient segment. Concentr...

Embodiment 2

[0028] Add 10 kg of water (containing 400 g of ammonia water) into an airtight container, heat to boil, put in 2 kg of fresh ginkgo leaves, and steam for 5 minutes to inactivate the enzyme. Take out the leaves, extract once with 90% ethanol at 70°C, filter, extract the filter residue once with 80% ethanol at 70°C, filter, combine the extracts, concentrate to a specific gravity of 1.1, adjust to neutral, place and filter, and mix the supernatant into 1 / 3 times the amount of polyamide dry. Take another 2.5kg of polyamide and 0.5kg of alumina to pack into the column, rinse with petroleum ether: ethyl acetate = 3:2, add the above-mentioned dried sample to the top of the column, and continue to wash with petroleum ether: ethyl acetate = 3:2 Take off, observe on the plate, collect the active ingredient segment, after the elution of the lactone is completed, change to ethyl acetate: ethanol = 3:1 for elution, observe on the plate, and collect the active ingredient segment. Concent...

Embodiment 3

[0030] Add 10 kg of water (containing 400 g of sodium bicarbonate) into an airtight container, heat to boil, put in 2 kg of fresh ginkgo leaves, and cook for 15 minutes to inactivate the enzyme. Take out the leaves, extract once with 90% ethanol at 70°C, filter, extract the filter residue once with 80% ethanol at 70°C, filter, combine the extracts, concentrate to a specific gravity of 1.1, adjust to neutral, place and filter, and mix the supernatant into 1 / 3 times the amount of polyamide dry. Another 1.72kg of polyamide and 0.28kg of alumina were packed into a column, rinsed with petroleum ether: ethyl acetate = 3:2, the above-mentioned dried sample was added to the top of the column, and continued to be washed with petroleum ether: ethyl acetate = 3:2 Remove, observe on the spot plate, collect the active ingredient segment, after the lactone elution is complete, change to ethyl acetate: ethanol = 4:1 for elution, spot the plate for observation, and collect the active ingred...

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PUM

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Abstract

The invention provides a method for extracting and separating ginkgo flavonoids and ginkgolides from ginkgo leaves, comprising three steps: an enzyme inactivation process, an extraction process and a column purification process; the present invention uses alkaline water to cook fresh ginkgo leaves to inactivate enzymes, which can The enzymes in fresh ginkgo leaves are quickly inactivated, and the ginkgo flavonoids in the raw materials can be freed to the greatest extent by soaking in alkaline water. The residual alkaline water after cooking is added to the organic solvent for extraction, which is beneficial to the subsequent extraction. Improve product yield.

Description

technical field [0001] The invention belongs to the field of extraction and separation of flavonoids and lactones, and relates to a method for extracting ginkgo flavonoids and ginkgolides, in particular to a method for extracting and separating ginkgo flavonoids and ginkgolides from ginkgo leaves. Background technique [0002] Ginkgo biloba is a perennial woody plant, also known as ginkgo tree and Gongsun tree. Ginkgo biloba is one of the rare gymnosperms in the world. Its survival history can be traced back to 200 million years ago, and the tree is more than a thousand years old. It is known as a "living fossil". The chemical components of Ginkgo biloba mainly include flavonoids, terpenes, phenols, 25 effective elements and 17 amino acids. [0003] The flavonoid glycosides in Ginkgo biloba are composed of quercetin, kaempferol, isorhamnetin, myricetin, luteolin and single, di, and triglucosides of acetin, and the latter three substances account for a large proportion. Sma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/16A61K127/00
CPCA61K36/16A61K2236/10A61K2236/333A61K2236/39A61K2236/55
Inventor 王晓莹肖红郭文华张瑜
Owner SHAANXI JIAHE PHYTOCHEM
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