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Rice yellow-green leaf mutation gene YGL8, protein coded by rice yellow-green leaf mutation gene YGL8, and application of rice yellow-green leaf mutation gene YGL8

A technology for mutating genes and yellow-green leaves, applied in the field of rice yellow-green leaf mutant gene YGL8, can solve problems such as yield reduction, abnormal plant growth and development, and decreased photosynthetic efficiency

Inactive Publication Date: 2016-03-23
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor traits of most seedling markers and the decline in photosynthetic efficiency, the plant growth and development are not normal and the yield is reduced, so its application in breeding practice is limited.

Method used

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  • Rice yellow-green leaf mutation gene YGL8, protein coded by rice yellow-green leaf mutation gene YGL8, and application of rice yellow-green leaf mutation gene YGL8
  • Rice yellow-green leaf mutation gene YGL8, protein coded by rice yellow-green leaf mutation gene YGL8, and application of rice yellow-green leaf mutation gene YGL8
  • Rice yellow-green leaf mutation gene YGL8, protein coded by rice yellow-green leaf mutation gene YGL8, and application of rice yellow-green leaf mutation gene YGL8

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1, Obtaining and Morphological Observation of Rice Yellow-green Leaf Mutant ygl8

[0019] In the rice EMS mutant bank of the Rice Research Institute of Southwest University, a mutant with yellow-green leaves during the whole growth period was found, and it was named ygl8 according to the phenotypic characteristics ( figure 1 ). This trait has been observed through multiple generations, showing stable inheritance. The ultrastructure of the leaf cells of the wild type Jinhui 10 and the ygl8 mutant was observed by transmission electron microscopy. figure 2 ); major agronomic traits such as panicle length and grain number per panicle were significantly reduced, however, there was no significant difference in thousand-grain weight ( image 3 ).

Embodiment 2

[0020] Embodiment 2, mutation ygl8 gene genetic analysis and localization

[0021] When the phenotypically normal sterile line Xinong1A (Xinong1A) was crossed with the ygl8 mutant, all F 1 The plants were all phenotypically normal. f 2 There were obvious segregation phenomenon of parental traits in the population. Among the 9473 populations, there were 7095 normal plants and 2378 yellow-green mutant plants. The chi square test showed that the segregation ratio was 3:1, indicating that the mutant trait was affected by a pair of recessive genes. control.

[0022] Preliminary mapping: 400 pairs of SSR markers evenly distributed on the 12 rice chromosomes were selected to amplify the parental and gene pool DNA. It was found that the markers RM6141, RM12233 and RM6321 located on the first chromosome may be linked to YGL8. Further analysis of F using linkage markers RM6141, RM12233 and RM6321 2 452 individuals with recessive mutations were found in the generation population, and...

Embodiment 3

[0027] Embodiment 3, clone Os01g73450 gene

[0028] According to the rice Nipponbare gene Os01g73450 sequence registered in GenBank, use VectorNTI software to design and amplify mRNA-specific primers for wild-type Jinhui 10 and ygl8 mutant Os01g73450 sequence: upstream primer YGL8-F: 5'-gtcctcatcgtcatcatctcgt-3' (SEQIDNo. 13); downstream primer YGL8-R: 5'-agcaataggcaatgcagctac-3' (SEQ ID No. 14). Take 2 g of young leaves of wild-type Jinhui 10 and mutant ygl8 cultured in light for two weeks, put them into liquid nitrogen quickly and grind them into powder, and extract total RNA according to the instructions of the Trizol kit. The results of electrophoresis of total RNA of wild-type Jinhui 10 and mutant ygl8 showed that the main band was clear and complete, and the band brightness ratio of 28S and 18S was about 2:1, indicating that the concentration and purity of RNA met the experimental requirements and could be used for synthesis double-stranded cDNA. Then use the obtained ...

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Abstract

The invention discloses a rice yellow-green leaf mutation gene YGL8, protein coded by the rice yellow-green leaf mutation gene YGL8, and application of the rice yellow-green leaf mutation gene YGL8. The nucleotide sequence of the rice yellow-green leaf mutation gene YGL8 is shown in SEQ ID No.11, and the amino acid sequence of the rice yellow-green leaf mutation gene YGL8 is shown in SEQ ID No.12. Compared with the wild type, according to the rice yellow-green leaf mutation gene YGL8, the base at the 671st position of an encoding frame is converted into T from C, and therefore the coding amino acid at the 224th position is varied to valine (Val) from alanine (Ala), by means of the rice YGL8 mutant obtained through gene mutation, all leaves are in the yellow green color during the whole growth period, it is found through genetic analysis that the character is the recessive character, field purification and seed purity authentication can be conducted with the character serving as the molecular marker, and important significance in genetics and breeding of rice is achieved.

Description

technical field [0001] The invention belongs to the field of genetics, and specifically relates to a rice yellow-green leaf mutation gene YGL8, and also relates to a protein encoded by the gene and its application. Background technique [0002] Rice (Orvza sativa L.) is the most important food crop in the world and is widely planted in 120 countries and regions. More than half of the world's population uses rice as a staple food. my country has successfully bred hybrid rice and promoted and applied it. The increased grain production has created conditions for some countries to achieve food self-sufficiency, and has made outstanding contributions to the food security of the country and the world. However, the hybrid seeds mixed due to the unstable fertility of sterile lines seriously affect the purity of hybrids and the production of hybrid rice, and even cause serious losses to farmers, restricting hybrid rice from playing a greater role. In the breeding and production prac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N9/12C12Q1/68
CPCC12N9/1229C12Q1/6895C12Q2600/13C12Y207/04022
Inventor 何光华朱小燕张天泉郭爽杜青王忠伟邢亚迪桑贤春凌英华
Owner SOUTHWEST UNIV
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