Preparation method of multi-dendritic colloidal gold nanoparticles with controllable particle size and high light absorption intensity

Abstract

The invention relates to the field of development and application of nanomaterials, and discloses a method for synthesizing multi-branched colloidal gold nanoparticles with different particle sizes and high light absorption intensity through growth mediated by colloidal gold seeds. First, the conventional trisodium citrate reduction method is used to reduce the Au3+ in the chloroauric acid molecule to Au0 to obtain spherical colloidal gold seeds. Then add colloidal gold seeds into a growth solution, the main components of the growth solution include ultrapure water, chloroauric acid and trisodium citrate and other solutions, stir slowly on a magnetic stirrer, and heat the solution to 50°C after mixing At this time, speed up the rotation speed, and at the same time, add a sufficient amount of hydroquinone solution at one time. The temperature of the mixed solution is maintained at 50 ° C. After continuing the reaction for 10 minutes, stop heating, and cool to room temperature to obtain a multi-dendritic colloidal gold solution with high absorbance. The method of the invention has the characteristics of easy operation, simple process, rapid response, good controllability, high repeatability and the like.

Description

technical field

[0001] The invention relates to the development of noble metal nanoparticles in the field of nanomaterial development and application, in particular to a method for synthesizing multi-branched colloidal gold nanoparticles with different particle sizes and high light absorption intensity through colloidal gold seed-mediated growth. Background technique

[0002] Colloid gold (colloid gold), also known as nanogold (nanogold), refers to the gold sol whose dispersed phase particle diameter is between 1-150nm, and belongs to the heterogeneous heterogeneous system. Colloidal gold particles consist of a basic gold core (Au atom) surrounded by a double ion layer, such as figure 1 As shown, the inner layer of negative ions is closely connected to the surface of the gold core, and the outer layer of ions is dispersed in the colloidal solution to maintain the suspension state of the colloidal gold free in the sol. The color of colloidal gold solution depends on the part...

Examples

Embodiment 1

[0027] Example 1 The particle size is 33 ± 3nm, the preparation of high absorbing intensity polydendric colloidal gold nanoparticles

[0028] Using spherical colloidal gold nanoparticles with a particle size of 18±2nm as seeds:

[0029] (1) Synthesis of spherical colloidal gold seed solution (spherical colloidal gold nanoparticles): using the classic trisodium citrate reduction method, accurately pipette 1mL of 1% HAuCl 4 Put the solution into a conical flask filled with 99mL ultrapure water, stir slowly on a magnetic stirrer and heat to boiling, then increase the speed and quickly add 2.7mL 1% Na at one time 3 C 6 h 5 o 7 Solution, continue to boil for 15min, until the color of the solution turns wine red and no longer changes, stop heating, stir at a constant speed and cool to room temperature, to obtain a spherical colloidal gold seed solution ( image 3 A). Such as figure 2 , the maximum surface plasmon resonance absorption wavelength was determined to be 520nm, the...

Embodiment 2

[0033] Example 2 Construction of Immunochromatographic Test Strips Based on High Absorbance Intensity and Low Absorbance Intensity Polydendrified Colloidal Gold Nanoparticles

[0034] Ochratoxin (OTA) was used as the analyte in a competitive inhibition mode. The test strip is composed of a sample pad, a binding pad, a nitrocellulose membrane, absorbent paper and a PVC bottom plate. The nitrocellulose membrane is coated with an ochratoxin antigen and a donkey anti-mouse secondary antibody as the detection line (T) and quality respectively. Control line (C):

[0035] (1) Particle size is 33 ± 3nm, high absorbing intensity polydendrite colloidal gold nanoparticle immunochromatographic test strip construction: be 33 ± 3nm with particle diameter, high absorbing intensity polydendric colloidal gold nanoparticle (take implementation The polydendritic colloidal gold solution with high absorbing intensity obtained in Example 1, without concentration treatment) is used as a labeling ma...

Embodiment 3

[0040] Example 3 The particle size is 68 ± 2nm, and the preparation of high absorbing intensity multi-dendrite colloidal gold nanoparticles

[0041] Using spherical colloidal gold nanoparticles with a particle size of 18±2nm as seeds:

[0042] (1) Synthesis of spherical colloidal gold seed solution (spherical colloidal gold nanoparticles): using the classic trisodium citrate reduction method, accurately pipette 1mL of 1% HAuCl 4 Put the solution into a conical flask filled with 99mL ultrapure water, stir slowly on a magnetic stirrer and heat to boiling, then increase the speed and quickly add 2.7mL 1% Na at one time3 C 6 h 5 o 7 Solution, continue to boil for 15min, until the color of the solution turns wine red and no longer changes, stop heating, stir at a constant speed and cool to room temperature, to obtain a spherical colloidal gold seed solution ( image 3 A). Such as figure 2 , the maximum surface plasmon resonance absorption wavelength was determined to be 520nm...

technical field

[0001] The invention relates to the development of noble metal nanoparticles in the field of nanomaterial development and application, in particular to a method for synthesizing multi-branched colloidal gold nanoparticles with different particle sizes and high light absorption intensity through colloidal gold seed-mediated growth. Background technique

[0002] Colloid gold (colloid gold), also known as nanogold (nanogold), refers to the gold sol whose dispersed phase particle diameter is between 1-150nm, and belongs to the heterogeneous heterogeneous system. Colloidal gold particles consist of a basic gold core (Au atom) surrounded by a double ion layer, such as figure 1 As shown, the inner layer of negative ions is closely connected to the surface of the gold core, and the outer layer of ions is dispersed in the colloidal solution to maintain the suspension state of the colloidal gold free in the sol. The color of colloidal gold solution depends on the part...

Examples

Embodiment 1

[0027] Example 1 The particle size is 33 ± 3nm, the preparation of high absorbing intensity polydendric colloidal gold nanoparticles

[0028] Using spherical colloidal gold nanoparticles with a particle size of 18±2nm as seeds:

[0029] (1) Synthesis of spherical colloidal gold seed solution (spherical colloidal gold nanoparticles): using the classic trisodium citrate reduction method, accurately pipette 1mL of 1% HAuCl 4 Put the solution into a conical flask filled with 99mL ultrapure water, stir slowly on a magnetic stirrer and heat to boiling, then increase the speed and quickly add 2.7mL 1% Na at one time 3 C 6 h 5 o 7 Solution, continue to boil for 15min, until the color of the solution turns wine red and no longer changes, stop heating, stir at a constant speed and cool to room temperature, to obtain a spherical colloidal gold seed solution ( image 3 A). Such as figure 2 , the maximum surface plasmon resonance absorption wavelength was determined to be 520nm, the...

Embodiment 2

[0033] Example 2 Construction of Immunochromatographic Test Strips Based on High Absorbance Intensity and Low Absorbance Intensity Polydendrified Colloidal Gold Nanoparticles

[0034] Ochratoxin (OTA) was used as the analyte in a competitive inhibition mode. The test strip is composed of a sample pad, a binding pad, a nitrocellulose membrane, absorbent paper and a PVC bottom plate. The nitrocellulose membrane is coated with an ochratoxin antigen and a donkey anti-mouse secondary antibody as the detection line (T) and quality respectively. Control line (C):

[0035] (1) Particle size is 33 ± 3nm, high absorbing intensity polydendrite colloidal gold nanoparticle immunochromatographic test strip construction: be 33 ± 3nm with particle diameter, high absorbing intensity polydendric colloidal gold nanoparticle (take implementation The polydendritic colloidal gold solution with high absorbing intensity obtained in Example 1, without concentration treatment) is used as a labeling ma...

Embodiment 3

[0040] Example 3 The particle size is 68 ± 2nm, and the preparation of high absorbing intensity multi-dendrite colloidal gold nanoparticles

[0041] Using spherical colloidal gold nanoparticles with a particle size of 18±2nm as seeds:

[0042] (1) Synthesis of spherical colloidal gold seed solution (spherical colloidal gold nanoparticles): using the classic trisodium citrate reduction method, accurately pipette 1mL of 1% HAuCl 4 Put the solution into a conical flask filled with 99mL ultrapure water, stir slowly on a magnetic stirrer and heat to boiling, then increase the speed and quickly add 2.7mL 1% Na at one time3 C 6 h 5 o 7 Solution, continue to boil for 15min, until the color of the solution turns wine red and no longer changes, stop heating, stir at a constant speed and cool to room temperature, to obtain a spherical colloidal gold seed solution ( image 3 A). Such as figure 2 , the maximum surface plasmon resonance absorption wavelength was determined to be 520nm...

Images