Pentachlorophenol monoclonal antibody hybridoma cell strain 2C3 and application thereof

Abstract

The invention discloses a pentachlorophenol monoclonal antibody hybridoma cell strain 2C3 and application thereof, and belongs to the field of food safety immunological detection. The pentachlorophenol monoclonal antibody hybridoma cell strain 2C3 is collected in the China General Microbiological Culture Collection Center (CGMCC) with a collection number CGMCC No.10871. A preparation method of the hybridoma cell strain 2C3 comprises the following steps: mixing a pentachlorophenol complete antigen and an equivalent Freund's adjuvant, and emulsifying the mixture, and immunizing a BALB / c mouse through back subcutaneous injection, wherein a complete Freund's adjuvant is used in a first immunizing process, and an incomplete Freund adjuvant is used afterwards; fusing spleen cells of the efficient and low-cost IC50 (Half Maximal Inhibitory Concentration) mouse with myeloma cells of the mouse through a PEG (Polyethylene Glycol) method, and performing indirect-competitive ELISA (Enzyme-Linked Immuno Sorbent Assay) screening and three times of sub-cloning to obtain a hybridoma cell strain 2C3. A monoclonal antibody secreted by the cell stain has high specificity and detection sensitivity (the IC50 value is 0.2mumg / L) specific to pentachlorophenol, and can be used for detecting residual pentachlorophenol in food safety.

Description

technical field [0001] The invention relates to a pentachlorophenol monoclonal antibody hybridoma cell line 2C3 and an application thereof, belonging to the field of food safety immunology detection. Background technique [0002] Pentachlorophenol, also known as pentachlorophenol, has a wide range of uses. As a pesticide, it is mainly its sodium salt. It can be used as a fungicide, preservative, herbicide, wood preservative, and partly as a solvent, heat exchange Waste from industrial production is released into the natural environment. Since the 1950s, my country has used pentachlorophenol and its sodium salt as the main pesticide to kill snails, the intermediate host of schistosomiasis. In 11 provinces, municipalities and autonomous regions in the middle and lower reaches of the Yangtze River in my country, a large area of ​​rice fields and ponds It has been continuously applied for decades, and its application was not gradually controlled until the 1980s. However, due to...

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Problems solved by technology

[0003] The method that detects pentachlorophenol at present mainly is gas chromatography (GC), liquid chromatography (HPLC), instrument methods such as spectrophotometry, derivative synchronous fluorescence method, thin-layer chromatography-spectrophotometry, gas chromatography-mass spectrometry, but These methods have the disadvantages of cumbersome operation, time-consuming, and high cost, and cannot realize the rapid detection of a large number of samples. Therefore, it is of great significance to establish a fast and simple pentachlorophenol detection method.

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