Acellularized scaffold type bioreactor for artificial liver and utilization method of acellularized scaffold type bioreactor for artificial liver

Abstract

The invention discloses an acellularized scaffold type bioreactor for an artificial liver and a utilization method of the acellularized scaffold type bioreactor for the artificial liver. The acellularized scaffold type bioreactor comprises a bioreactor shell, an acellularized scaffold, a filling scaffold, liquid inlets and a liquid outlet. The bioreactor shell comprises an upper portion and a lower portion, each of the upper portion and the lower portion is in a hollow structure, and a first cavity in the upper portion and a second cavity in the lower portion are in threaded connection to form a cylindrical inner cavity. A middle region of the top of the upper portion is provided with an inner cavity liquid outlet, and a center region of the bottom of the lower portion is provided with an inner cavity main liquid inlet. The main liquid inlet is branched into a first liquid inlet and a second liquid inlet, the first liquid inlet is connected to a liquid inlet of the acellularized scaffold, and the second liquid inlet is connected to the filling scaffold. By the acellularized scaffold type bioreactor for the artificial liver, in-vivo perfusion can be simulated, and uniformity in perfusion and reduction of dead spaces are realized; shear force applied to liver cells can be reduced, a three-dimensional growing environment for the liver cells can be simulated, and the amount of the liver cells in the bioreactor is increased.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a medical device, in particular to a decellularized scaffold bioreactor for a biological artificial liver or a hybrid artificial liver and a method for using the same. Background technique [0002] Conservative medical treatment of liver failure is not effective, and the mortality rate is high. Artificial liver is an important treatment for liver failure, which includes non-biological artificial liver, biological artificial liver and hybrid artificial liver: non-biological artificial liver can only be used to a certain extent. It temporarily compensates the detoxification effect of the liver, but cannot completely replace the synthesis, metabolism and biotransformation functions of the liver, so it has no obvious effect in improving the long-term prognosis of patients with chronic liver failure, so it is closer to the physiological function of the human liver. Liver and hybrid ...

AI Technical Summary

Problems solved by technology

[0002] Conservative medical treatment of liver failure is not effective, and the mortality rate is high. Artificial liver is an important treatment for liver failure, which includes non-biological artificial liver, biological artificial liver and hybrid artificial liver: non-biological artificial liver can only be used to a certain extent. It temporarily compensates the detoxification effect of the liver, but cannot completely replace the synthesis, metabolism and biotransformation functions of the liver, so it has no obvious effect in improving the long-term prognosis of patients with chronic liver failure, so it is closer to the physiological function of the human liver. Liver and hybrid artificial liver are currently research hotspots; bioreactors provide a good living environment for liver cells in vitro, and are places where plasma and liver cells of the treated subjects exchange substances. Core device; the hollow fiber bioreactor is the most researched bioreactor so far, but it has disadvantages such as uneven cell distribution, low cell adhesion rate and poor activity, and semi-permeable membrane blockage, so people are constantly developing various new bioreactors. Reactor; with the development of tissue engineering technology and material science, it has been found that a variety of artificial synthetic materials, such as galactose-modified macroporous chitosan scaffolds, can better promote the growth and function of liver cells in vitro; animal experiments suggest that, The construction of scaffold bioreactors based on these artificial materials has a certain effect on liver failure. However, these scaffold bioreactors also have many defects, which restrict their curative effect, such as easy formation of dead space and dead space. , uneven liquid flow, large liquid flow resistance, etc.; in recent years, it has been found that the liver tissue is decellularized and the lumen of the vascular knot tissue is preserved, thereby becoming a biological scaffold. After reimplanting liver cells from the portal vein system, and by The portal system is perfused with culture fluid, and the fluid can be perfused along the preserved vascular lumen; and this type of scaffold can provide liver cells with a three-dimensional growth environment that simulates the growth in vivo, thereby significantly improving the function of liver cells; the "tissue engineered artificial liver" Transplanted into animals with liver failure, shows good therapeutic effect

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