Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of hyaluronic acid odd oligosaccharide monomer and its preparation method

A technology of hyaluronic acid and oligosaccharide is applied in the field of biomedicine to achieve the effects of easy industrialization, overcoming the difficulty of desalination and improving efficiency

Active Publication Date: 2018-04-03
OCEAN UNIV OF CHINA
View PDF15 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there are still no relevant reports on the method of preparing a series of odd-numbered hyaluronic acid oligosaccharide monomers with specific molecular weight and clear structure through a simple process and on a large scale.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of hyaluronic acid odd oligosaccharide monomer and its preparation method
  • A kind of hyaluronic acid odd oligosaccharide monomer and its preparation method
  • A kind of hyaluronic acid odd oligosaccharide monomer and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Preparation of hyaluronic acid odd-numbered oligosaccharide monomer by dilute acid degradation

[0038] The dilute acid degradation preparation method of hyaluronic acid odd oligosaccharide monomer described in this embodiment comprises the following steps:

[0039] 1. Take 75g of hyaluronic acid with a molecular weight of 600 kDa and add it into 1500mL of distilled water, heat and stir to make it dissolve completely, add sulfuric acid to make the final concentration reach 0.01mol / L~2mol / L (the volume unit of adding dilute acid in the present invention is mL, The unit of mass of hyaluronic acid is g), 60 ℃ ~ 100 ℃ water bath stirring and hydrolysis for 0.5h ~ 5h, after the reaction is completed, the reaction solution is cooled to room temperature, the pH value of the solution is adjusted to neutral with sodium hydroxide, and 3 times the volume of ethanol is added After centrifugation, the supernatant was concentrated under reduced pressure and vacuum, and fre...

Embodiment 2

[0042] Example 2: Preparation of hyaluronic acid odd-numbered oligosaccharide monomer by solid-phase acid degradation

[0043] The solid-phase acid degradation preparation method of hyaluronic acid odd-numbered oligosaccharide monomers described in this example comprises the following steps:

[0044] 1. Take 200g of hyaluronic acid with a molecular weight of 200kDa and add it into 2000mL of distilled water, heat and stir to dissolve it completely, add 400g of polystyrene type strong cation exchange resin, react in a water bath at 50℃~90℃ for 1h~8h, filter, and cool the filtrate to room temperature Potassium hydroxide was used to adjust the pH value of the solution to neutrality, 4 times the volume of methanol was added and centrifuged, the supernatant was concentrated under reduced pressure and vacuum, and freeze-dried to obtain a hyaluronic acid-oligosaccharide mixture.

[0045] 2. After dissolving the oligosaccharide mixture in water, separate it with a Bio-Gel P6 gel chroma...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
degree of polymerizationaaaaaaaaaa
Login to View More

Abstract

The invention provides a hyaluronan odd oligosaccharide monomer and its preparation method. Polymerization degree of the hyaluronan odd oligosaccharide monomer is 3-23. The preparation method comprises the following steps: degrading hyaluronan with acid or hydrogen cation exchange resin, neutralizing with alkali, concentrating, precipitating with an organic solvent, centrifuging, concentrating a supernatant, and finally separating by gel column choromatography so as to obtain hyaluronan odd oligosaccharide monomers of different polymerization degrees. By one-time separation, high-purity hyaluronan odd oligosaccharide monomers with polymerization degree being 3-23 can be prepared. In addition, the product has advantages of novel structure, small molecular weight, good dissolvability and stable performance. The preparation technology is simple, low-cost and pollution-free, and is easy for industrialization. The hyaluronan odd oligosaccharide monomer obtained in the invention can be used in preparation of an oligosaccharide standard reagent or can be used as a bioactive substance or an intermediate to further prepare an oligosaccharide health product or an oligosaccharide drug. The product is fully utilized, and its function and activity are widely developed.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a hyaluronic acid odd-numbered oligosaccharide monomer and a preparation method thereof. Background technique [0002] Hyaluronic acid (HA) is a disaccharide repeat unit composed of N-acetylglucosamine (GlcNAc) and D-glucuronic acid (GlcA) through β-(1→4) glycosidic bonds and β-(1→ 3) Unbranched macromolecular acidic mucopolysaccharides composed of glycosidic bonds are widely distributed in the intercellular matrix of animal tissues and the capsule of some bacteria. With the deepening of research on the distribution, chemical structure and physical and chemical properties of HA at home and abroad, it has been found that HA has many biological functions, such as participating in lung and vascular diseases, signal transduction of embryogenesis, wound healing, etc., making it widely used in food. , medicine and cosmetics and other fields have extensive and unique ap...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08C13K13/00
CPCC08B37/0072C13K13/00
Inventor 于广利赵小亮李国云蔡超
Owner OCEAN UNIV OF CHINA