A strain of Oleococcus graesiella sp.WBG-1 and its isolation and screening method and application

Abstract

The invention discloses a strain of Oleococcus Graesiella sp.WBG‑1 and its separation and screening methods and applications. The algal strain grows fast, has strong adaptability to pH changes, and has the characteristics of large cells, easy sedimentation, and resistance to protozoan ingestion, which are suitable for large-scale cultivation. Oleococcus Graesiella sp.WBG‑1 accumulates a large amount of oil in cells under low-nitrogen culture conditions, which can be used as a raw material for microalgae biodiesel, and accumulates a large amount of protein in cells under high-nitrogen culture conditions, which can be used for the production of microalgae protein. Under culture, it can efficiently fix exogenous carbon dioxide, and can be applied to the biological fixation of flue gas carbon dioxide.

Description

technical field [0001] The present invention relates to the fields of microalgae resources, energy and environmental protection, more specifically to a strain of Oleococcus Graesiellasp. Use of Oleococcus Graesiella sp.WBG-1 in microalgae biodiesel feedstock, microalgae protein production, and microalgae fixation of exogenous carbon dioxide. Background technique [0002] Microalgae are widely distributed in nature and can produce a variety of metabolites. Separation and screening of fine algae species, development of new uses of microalgae and new production processes are the main tasks of microalgae biotechnology research. In the 1960s, Japan realized the large-scale cultivation and production of Chlorella (Tsukada and Kawahara, 1977, Biological Solar Energy Conversion 363-365). So far, Spirulina (Shimamatsu, 2003, Hydrobiologia512:39-44), Dunaliella (Oren, 2005, AquaticBiosystems 1 (1): 2-15), Haematococcus (Boussiba and Vonshak, 1991, Plant & Cell Physiology 32 (7): 10...

AI Technical Summary

Problems solved by technology

[0004] Nevertheless, there is no successful case of commercial production of microalgal biomass fuel so far (Steenberghen and López, 2008, Journal of Cleaner Production 16(5):577-590; Stephens, Ross et al., 2010, Trends in Plant Science; Wijffels and Barbosa, 2010, Science 329 (5993): 796-799), there are three aspects to the bottleneck problem that causes this present situation: 1, the high cost of microalgae biodiesel has seriously hindered the development of microalgae biofuel Commercial production (Torzillo, Pushparaj et al., 2003, Biotechnology and Bioprocess Engineering 8(6):338-348), 2. The basic understanding of genetic regulation and physiological regulation of oil synthesis and accumulation in microalgae is not deep, and microalgae scale The engineering technology foundation cultivated is weak, etc. (Wijffels and Barbosa, 2010, Science 329(5993): 796-799; Li Jian, Zhang Xuecheng, etc., 2012, Science Bulletin 1(1): 23-31); 3. Lack of easy-to-cultivate, High-quality algal species that grow fast, have high environmental tolerance, and are suitable for large-scale cultivation (Beal, Smith et al., 2011, Bioenergy research 4(1): 36-60; Liu, Chen et al., 2011, Progress in Natural Science: Materials International 21(4):269-276; Li Tao, Li Aifen et al., 2011, China Biotechnology Journal 31(004):98-105; Li, Pribyl et al., 2013, Biotechnology and Bioengineering 110(1):97-107 ; Guccione, Biondi et al., 2014, Biotechnology for Biofuels 7(1):84)

Searching for high-quality algae species from abundant natural microalgae resources is expected to solve the problems of low yield, easy pollution, high harvesting energy consumption, and poor environmental adaptability encountered in the current large-scale cultivation of microalgae. Cost efficient approach to material production (Stephens, Ross et al., 2010, Trends in Plant Science; Wijffels and Barbosa, 2010, Science 329(5993):796-799)

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