Preparation method and application of fluorescent gold nanoparticles

Abstract

The invention provides a preparation method and application of fluorescent gold nanoparticles. Preparation of fluorescent gold nanoparticles: in parts by volume, 0.2-1.0 parts of 100mmol / L N-acetyl-L-cysteine ​​aqueous solution, 0.2-1.0 parts of 100mmol / L chloroauric acid aqueous solution and 0.5-5.0 parts by volume Methanol / glacial acetic acid solution with a ratio of 6:1, stirred for 1-60min, then added 3.8-8.3 parts of ultrapure water to the above mixed solution, stirred and heated at 50-90°C, refluxed for 2-48h, and waited After cooling, it was taken out, and after dialysis and drying, fluorescent gold nanoparticles were obtained. The invention has a simple preparation process, avoids the use of the highly toxic reducing agent sodium borohydride, simple reaction conditions, and is environmentally friendly, and the prepared gold nanoparticles have a high fluorescence quantum yield, a large Stocks shift value, and a long fluorescence lifetime. It has good stability and can be used for the detection of mercury ions and cell imaging.

Description

technical field [0001] The invention relates to the preparation of gold nanoparticles, in particular to a preparation method and application of core-shell water-soluble fluorescent gold nanoparticles. Background technique [0002] The harm of heavy metal pollution to the ecological environment is increasing day by day, and the phenomenon of heavy metal poisoning is not uncommon. Various heavy metals are poisoning the human body and mind, especially the harm of Hg, Pb, Cd and Cu cannot be ignored. Although the concentration of heavy metal ions in the environment is not large, However, once they are released into the environment, they are not easy to be removed, and accumulate in the environment for a long time, directly or indirectly posing a threat to human health and the survival of various organisms. Therefore, it has become a hotspot in the field of analytical chemistry to develop a new, easy-to-operate, and easy-to-handle method. In addition, the rapid development of li...

AI Technical Summary

Problems solved by technology

In our previously reported work (Analytical Chemistry, 2009, 81(4), 1676–1695; Journal of Analytical Science, 2013, 29(5): 599-604.) with N-acetyl-L-cysteine, Chlorauric acid is reactant, and when methanol / glacial acetic acid is solvent to prepare gold nanoparticles, need to add strong reductant sodium borohydride to reduce the trivalent gold in the chloroauric acid to zero-valent gold, regenerate gold nanoparticles, reaction needs minute Step by step, and the strong reducing agent sodium borohydride used in the reaction strongly irritates mucous membranes, upper respiratory tract, eyes and skin, and can cause death due to spasm, inflammation and edema of the larynx and bronchi, chemical pneumonia and pulmonary edema after inhalation, toxicity bigger

Moreover, the fluorescence quantum yield and fluorescence lifetime of the prepared gold nanoparticles are short, which limits its application range.

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