Method for preparing high maltose oligosaccharide
A maltose and maltotriose technology, which is applied in the field of starch sugar derivative preparation, can solve the problems of complex activated carbon adsorption and alcohol elution process, unfavorable for industrial production and environmental protection, etc. Produce, stable performance
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Embodiment 1
[0029] A method for preparing high maltoligosaccharides, comprising the following steps:
[0030] a) Using starch as raw material, adjust starch concentration at 16-17 Baume, adjust pH=5.5, temperature 90°C, add ɑ-high temperature amylase, after liquefaction reaction, starch will generate small molecule dextrin; ɑ-high temperature starch The amount of enzyme added is 150mL / T.DS
[0031] After the starch is liquefied, the sugar liquid is saccharified by the joint action of maltotriose, barley β-amylase and pullulanase to produce a product with a maltose and maltotriose content of about 40%; maltotriose 450mL / T.DS, barley The amount of β-amylase 150mL / T.DS and pullulanase added is 300mL / T.DS, and the saccharification conditions are: pH=4.5-5.1, temperature 58-60°C;
[0032] b) The concentrated sugar solution after saccharification is purified by chromatographic separation technology, the mass fraction of the feed is 55%, and the operating temperature of the system is 60°C, the ...
Embodiment 2
[0037] A method for preparing high maltoligosaccharides, comprising the following steps:
[0038] a) Using starch as raw material, adjust starch concentration at 16-17 Baume, adjust pH=6.0, temperature 100°C, add ɑ-high temperature amylase, after liquefaction reaction, starch will generate small molecule dextrin; ɑ-high temperature starch The amount of enzyme added is 150mL / T.DS
[0039] After the starch is liquefied, the sugar liquid is saccharified by the combined action of maltotriose, barley β-amylase and pullulanase to produce a product with a maltose and maltotriose content of about 40%; maltotriose 600mL / T.DS, barley The amount of β-amylase 150mL / T.DS and pullulanase added is 300mL / T.DS, and the saccharification conditions are: pH=pH=4.5-5.1, temperature 58-60°C;
[0040] b) The concentrated sugar liquid after saccharification is purified by chromatographic separation technology, the mass fraction of the feed is 60%, and the operating temperature of the system is 60°C,...
Embodiment 3
[0045] A method for preparing high maltoligosaccharides, comprising the following steps:
[0046] a) Using starch as raw material, adjust starch concentration at 16-17 Baume, adjust pH=6.5, temperature 110°C, add ɑ-high temperature amylase, after liquefaction reaction, starch will generate small molecule dextrin; ɑ-high temperature starch The amount of enzyme added is 150mL / T.DS liquefies the starch and saccharifies the sugar solution through the joint action of maltotriose, barley β-amylase and pullulanase to produce a product with a maltose and maltotriose content of about 40%; The amount of trisaccharidase 450mL / T.DS, barley β-amylase 150mL / T.DS and pullulanase is 600mL / T.DS, and the saccharification conditions are: pH=pH=4.5-5.1, temperature 58-60 ℃;
[0047] b) The concentrated sugar liquid after saccharification is purified by chromatographic separation technology, the mass fraction of the feed is 60%, and the operating temperature of the system is 60°C, and the maltose...
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