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Albumin magnetic nanoparticles for magnetic resonance imaging (MRI) and preparation method thereof

A technology of magnetic nanoparticles and contrast imaging, which is applied in the field of biomedicine, can solve the problems of difficult separation and purification of products, complicated processes of ferric oxide nanoparticles, etc., and achieves the effect of simple and easy purification operation.

Active Publication Date: 2016-06-01
TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] In order to solve the disadvantages of complex process for preparing ferric oxide nanoparticles by co-precipitation method and the difficulty of separation and purification of the prepared products, we simplified the experimental steps in the traditional co-precipitation preparation method, and the hydrated particle size of the prepared nanoparticles was 50 ~90nm, relatively stable properties, simple purification method

Method used

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  • Albumin magnetic nanoparticles for magnetic resonance imaging (MRI) and preparation method thereof
  • Albumin magnetic nanoparticles for magnetic resonance imaging (MRI) and preparation method thereof
  • Albumin magnetic nanoparticles for magnetic resonance imaging (MRI) and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Weigh 1 g of bovine serum albumin and dissolve it in deperoxygenated water, and pre-gas nitrogen to remove the oxygen in the reaction system. First add dilute ammonia solution drop by drop to make the pH of the system 12, then drop by drop the precisely weighed mixed solution of anhydrous ferric chloride and ferrous sulfate, the molar ratio of ferric iron to ferrous iron is 1 : 1.8, the total concentration of iron is 1.8M / L, and the molar ratio of iron to albumin is 2.4×10 4 :1. Under the conditions of 1000rmp magnetic stirring and nitrogen protection, the reaction was completed after heating at 75° C. for 30 minutes. After the reaction, when the temperature of the solution drops to room temperature, the nanoparticles are dialyzed for 24 hours with a dialysis bag with a molecular weight of 8kD-14kD. During the dialysis process, the ultrapure water is changed 6 times to obtain pure albumin magnetic nanoparticles.

[0033] Get the purified albumin iron ferric oxide nano...

Embodiment 2

[0036] Weigh 0.6 g of bovine serum albumin and dissolve it in deperoxygenated water, and pre-flow nitrogen to remove oxygen in the reaction system. First add dilute ammonia solution drop by drop to make the pH of the system 10, then drop by drop the precisely weighed mixed solution of anhydrous ferric chloride and ferrous sulfate, the molar ratio of ferric iron to ferrous iron is 1 :2, the total concentration of iron is 1.4M / L, and the molar ratio of iron to albumin is 3.1×10 4 :1. Under the conditions of 700rmp magnetic stirring and nitrogen protection, the reaction was completed after heating at 65° C. for 60 minutes. After the reaction, when the temperature of the solution drops to room temperature, the nanoparticles are dialyzed for 24 hours with a dialysis bag with a molecular weight of 8kD-14kD. During the dialysis, the ultrapure water is changed 4 times to obtain pure albumin magnetic nanoparticles.

[0037] Get the purified albumin iron ferric oxide nanoparticles, af...

Embodiment 3

[0040] Weigh 0.8 g of bovine serum albumin and dissolve in peroxygenated water, and pre-flow nitrogen to remove oxygen in the reaction system. First add dilute ammonia solution drop by drop to make the pH of the system 11, then add drop by drop the precisely weighed mixed solution of anhydrous ferric chloride and ferrous sulfate, the molar ratio of ferric iron to ferrous iron is 1 : 1.9, the total concentration of iron is 1.6M / L, and the molar ratio of iron to albumin is 2.7×10 4:1. Under the conditions of 900rmp magnetic stirring and nitrogen protection, the reaction was completed after heating at 70° C. for 45 minutes. After the reaction, when the temperature of the solution dropped to room temperature, the nanoparticles were dialyzed for 24 hours with a dialysis bag with a molecular weight of 8kD-14kD. During the dialysis, the ultrapure water was changed 5 times to obtain pure albumin magnetic nanoparticles.

[0041] Get the purified albumin iron ferric oxide nanoparticle...

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Abstract

The invention relates to albumin magnetic nanoparticles for magnetic resonance imaging (MRI) and a preparation method thereof. The preparation method includes the steps of dissolving albumin in deoxidized water, leading in nitrogen gas to remove oxygen in a reaction system, adding in dilute ammonia solution dropwise to regulate pH value of the system to be 10-12, adding in anhydrous ferric chloride and ferrous sulfate mixed solution dropwise, stirring on a condition of nitrogen protection, raising heat to 65-75 DEG C for 30-60 minutes, and then cooling to the room temperature and subjecting the mixture to purification after finishing reaction, and finally dialyzing the prepared nanoparticles by dialysis bags with molecular weight of 8kD-14kD for 24 hours to obtain the pure albumin magnetic nanoparticles. In anhydrous ferric chloride and ferrous sulfate mixed solution, the total Fe concentration is up to 1.4-1.8M / L, and molar ratio of Fe to protein is equal to (2.4*104-3.1*104):1. The hydrodynamic size of the nanoparticles ranges from 50 micrometers to 90 micrometers, and thus the nanoparticles are adaptable to biological application such as MRI, drug carriers and immunoassay, and are perfect materials for biomedicine application.

Description

technical field [0001] The albumin magnetic nanoparticle belongs to the field of biomedicine, and relates to the MRI contrast imaging effect of the albumin magnetic nanoparticle and a preparation method thereof. Background technique [0002] Albumin is the most abundant protein in human plasma, and magnetic nanoparticles prepared with albumin as a stabilizer are highly biocompatible and non-toxic. This albumin magnetic nanoparticle has a wide range of in vivo applications, such as: MRI, immunoassay, drug delivery, tissue separation, cell targeting, etc., and is an ideal material for biomedical applications. The key factors for nanoparticles used in biomedicine are suitable particle size and convenient and simple purification process for industrial production. The commonly used synthetic methods are: (1) co-precipitation method; (2) high temperature reaction method; (3) electrochemical reaction method; (4) microemulsion method; (5) flow injection synthesis method; (6) polyol...

Claims

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Application Information

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IPC IPC(8): A61K49/14A61K49/18
CPCA61K49/143A61K49/1869
Inventor 贺慧宁梁秋玲刘二刚陈孙慧杨志民
Owner TIANJIN MEDICAL UNIV
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