A temperature-sensitive multi-block polymer and its preparation method and application
A block polymer, temperature-sensitive technology, used in medical science, surgery, etc., can solve the problems of low hydrophobicity, easy damage, loss of anti-adhesion of PPO segments, and achieve excellent biocompatibility, easy Degradation, molecular weight reduction effect
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Embodiment 1
[0050] Under nitrogen protection, mix L-lactide, 10R5 with a mass ratio of 1 and 0.3% stannous octoate catalyst based on the total mass of 10R5 and L-lactide, stir and heat up to 140°C for 10h, and cool to room temperature , then add dichloromethane to dissolve the reaction product, and then put it into petroleum ether at -20°C for precipitation, filter, and repeat the operation 2 to 3 times before drying; dissolve the dry product in deionized water, and then use dialysis The bag is dialyzed to remove small molecular monomers and excess catalysts, and the dialyzed multi-block polymer is freeze-dried and sealed for storage.
[0051] The infrared spectrogram of the PLA-10R5-PLA multi-component block polymer test obtained in this embodiment is shown in figure 1 b in the test; the NMR spectrum of the test is shown in image 3 ; Its theoretical molecular weight Mn a 4.0×10 3 ; b 4.02×10 3 ; c 5.26×10 3 ; c 4.33×10 3 . The collection of illustrative plates of this multi-co...
Embodiment 2
[0053] Under the protection of nitrogen, mix L-lactide, 10R5 with a mass ratio of 0.25 and 0.3% stannous octoate catalyst based on the total mass of 10R5 and L-lactide, stir and heat up to 130 ° C for 7 hours, and cool to room temperature , then add tetrahydrofuran to dissolve the reaction product, and then put it into petroleum ether at -20°C for precipitation, filter, repeat the operation 2 to 3 times, and then dry; dissolve the dry product in deionized water, and then use a dialysis bag for dialysis The small molecule monomer and excess catalyst are removed, and the multi-block polymer after dialysis is freeze-dried and stored in a sealed container.
[0054] Theoretical molecular weight Mn of PLA-10R5-PLA multi-component block polymer obtained in the present embodiment a 2.5×10 3 ; b 2.66×10 3 ; c is 3.76×10 3 ; c 2.59×10 3 .
Embodiment 3
[0056] Under nitrogen protection, mix L-lactide, 10R5 with a mass ratio of 0.75 and 0.3% stannous octoate catalyst based on the total mass of 10R5 and L-lactide, stir and heat up to 160°C for 5 hours, and cool to room temperature , then add dichloromethane and chloroform (1:1, v:v) to dissolve the reaction product, and then put it into petroleum ether at -20°C for precipitation, filter, and repeat the operation 2 to 3 times before drying; Dissolving the dried product in deionized water, and then using a dialysis bag to dialysis to remove small molecular monomers and excess catalyst, and the multi-component block polymer after dialysis is freeze-dried and sealed for storage.
[0057] Theoretical molecular weight Mn of PLA-10R5-PLA multi-component block polymer obtained in the present embodiment a 3.5×10 3 ; b is 3.68×10 3 ; c is 4.79×10 3 ; c is 3.61×10 3 .
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