Tiopronin concentration determination fluorescence sensor and preparation method thereof

A fluorescence sensor, tiopronin technology, applied in fluorescence/phosphorescence, instruments, measuring devices, etc., can solve the problem of weak research on thin-film fluorescence sensors, achieve regeneration and reuse, convenient and fast detection process, and simple preparation method. Effect

Inactive Publication Date: 2016-06-15
BEIJING UNIV OF CHEM TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, the research on thin-film fluorescent sensors is ...

Method used

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  • Tiopronin concentration determination fluorescence sensor and preparation method thereof
  • Tiopronin concentration determination fluorescence sensor and preparation method thereof
  • Tiopronin concentration determination fluorescence sensor and preparation method thereof

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Experimental program
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Embodiment 1

[0034] 1. Preparation of hydrotalcite precursor: 0.002mol solid Mg(NO 3 ) 2 ·6H 2 O, 0.01mol solid Al(NO 3 ) 3 9H 2 O and 0.012mol urea were dissolved in 70ml of deionized water, reacted at 100°C for 24 hours in a 90ml polytetrafluoroethylene-lined pressure reaction vessel, and were centrifugally washed with deionized water until the pH was about 7, 60 Dry in the air under the condition of ℃ to obtain the hydrotalcite microcrystalline Mg intercalated with carbonate-type anions 0.67 Al 0.33 (OH) 2 (CO 3 ) 0.33 2H 2 O. Get the hydrotalcite crystallite 0.3g of above-mentioned carbonate type anion intercalation and solid NaNO 3 63.75g dissolved in 300mL to remove CO 2 After being uniformly dispersed in deionized water, add 0.08mL concentrated nitric acid and stir at 25°C under a nitrogen atmosphere to carry out ion exchange reaction for 24 hours and then use 2 The deionized hot water was centrifuged and washed to a pH of about 7, and dried at 60°C for 12 hours to obta...

Embodiment 2

[0053] 1. Same as implementation case 1;

[0054] 2. Take 0.11 g of the above-mentioned nitrate intercalated hydrotalcite, and stir it in 110 mL of formamide solvent for 47 hours under a nitrogen atmosphere at a stirring speed of 2500 rpm, centrifuge the stripped hydrotalcite solution, discard the precipitate, Obtain clear transparent hydrotalcite laminate colloidal solution;

[0055] 3. The preparation concentration is 10 -3 mol / L alizarin red and boric acid mixed solution with a concentration of 0.15mol / L, after adjusting the pH value to 6.5 with NaOH, store it in a volumetric flask in the dark;

[0056] 4. Take a 3cm×1cm quartz plate and use concentrated H with a volume ratio of 7:3 2 SO 4 and H 2 o 2 Wash it with the mixed solution of ethanol and deionized water alternately for 2 times, each time for 15 minutes, then place it in a 19-fold diluted PDDA solution and soak it for 28 minutes, take it out, rinse it with deionized water and dry it at room temperature ; This...

Embodiment 3

[0060] 1. Same as implementation case 1;

[0061] 2. Take 0.15 g of the above-mentioned nitrate intercalated hydrotalcite, and stir it in 150 mL of formamide solvent for 46 hours under a nitrogen atmosphere, at a stirring speed of 2600 rpm, centrifuge the stripped hydrotalcite solution, discard the precipitate, A clear and transparent colloidal solution was obtained.

[0062] 3. The preparation concentration is 10 -3 mol / L alizarin red and boric acid mixed solution with a concentration of 0.25mol / L, after adjusting the pH value to 7.5 with NaOH, store it in a volumetric flask in the dark;

[0063] 4. Take a 3cm×1cm quartz plate and use concentrated H with a volume ratio of 7:3 2 SO 4 and H 2 o 2 Wash it with the mixed solution of ethanol and deionized water alternately for 18 minutes each time, then place it in a diluted 21-fold volume of PDDA solution for 25 minutes, take it out, rinse it with deionized water and dry it at room temperature ; This process is a pre-assemb...

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Abstract

The invention discloses a tiopronin concentration determination fluorescence sensor and a preparation method thereof. After hydrotalcite is delaminated, a laminate has positive charges, a guest molecule alizarin red-boric acid system has negative charges, and the hydrotalcite laminate and the guest molecule are self-assembled layer by layer through an electrostatic attraction layer to form a super-molecular structure system and then to be prepared into films; a molecular structure of boric acid is changed by adjusting the layer quantity of the thin films, the concentration of boric acid and the pH value of an alizarin red-boric acid solution system in an assembling process; and a fluorescence sensor thin film with high fluorescence intensity and high stability is obtained through optimized treatment. The fluorescence sensor thin film has the advantages that the preparation method is simple, the cost is low, and quantitative detection of tiopronin is realized. The fluorescence sensor provided by the invention can be used for detecting concentration of tiopronin conveniently and rapidly, and is high in sensitivity and low in detection limit; in the detection process, the sensor does not pollute a system to be detected, and has no reagent loss; and regeneration and repeated utilization of the sensor can be realized.

Description

technical field [0001] The invention belongs to the technical field of sensor preparation, in particular to a fluorescent sensor for measuring the concentration of tiopronin and a preparation method thereof. Background technique [0002] In the field of biochemistry, tiopronin, as a glycine derivative, has the functions of detoxification, protecting liver tissue and improving liver function. However, when the drug is overdose, it can cause adverse symptoms such as blood pressure drop and rapid breathing in a short period of time. Therefore, an efficient and rapid method for detecting the concentration of tiopronin is a hot topic in the field of biochemistry. For the detection of tiopronin, most manufacturers use titration and high-performance liquid chromatography. The specificity of titration is poor, while high-performance liquid chromatography is expensive and complicated to operate. According to the development needs of the medical industry, a more rapid, efficient and...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 靳兰吴海芹任和卫敏
Owner BEIJING UNIV OF CHEM TECH
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